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[1]陈剑,李君文,邱志刚,等.CTX-M型产ESBLs耐药基因在城市典型河流中的生态分布[J].应用与环境生物学报,2014,20(01):40-44.[doi:10.3724/SP.J.1145.2014.00040]
　CHEN Jian,LI Junwen,QIU Zhigang,et al.Ecological distribution of CTX-M extended-spectrum β-lactamase antibiotic resistant gene in typical city rivers of China[J].Chinese Journal of Applied & Environmental Biology,2014,20(01):40-44.[doi:10.3724/SP.J.1145.2014.00040]
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CTX-M型产ESBLs耐药基因在城市典型河流中的生态分布()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
20卷
期数:
2014年01期
页码:
40-44
栏目:
研究论文
出版日期:
2014-02-25

文章信息/Info

Title:
Ecological distribution of CTX-M extended-spectrum β-lactamase antibiotic resistant gene in typical city rivers of China
作者:
陈剑; 李君文; 邱志刚; 郭聪; 谌志强; 汲珊珊; 陈照立; 王新为; 金敏
1军事医学科学院卫生学环境医学研究所 天津 300050 2四川大学生命科学学院 成都 610064
Author(s):
CHEN Jian;  LI Junwen;  QIU Zhigang;  GUO Cong;  CHEN Zhiqiang;  JI Shanshan;  CHEN Zhaoli;  WANG Xinwei;  JIN Min
1 Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China 2 College of Life Sciences, Sichuan University, Chengdu 610064, China
关键词:
CTX-M型ESBL; 抗生素耐药基因; 宏基因组学; RFLP; 生态分布
Keywords:
CTX-M type of ESBL;  antibiotic resistant gene;  metagenomics;  RFLP;  ecological distribution
分类号:
X522
DOI:
10.3724/SP.J.1145.2014.00040
文献标志码:
A
摘要:
为了解流经我国城市的典型河流中CTX-M型耐药基因的分布情况及基因变异的多态性现状，进而为科学预防和避免抗生素耐药性的产生和传播提供生态学理论支持，采集我国东部6条典型河流流经城市区段的水样，采用宏基因组学技术与限制性酶切片段多态性分析（RFLP）技术相结合对质粒宏基因组PCR产物进行了分析，并将数据进行生态学统计处理. 结果显示CTX-M三种亚型的基因群落在调查中的6条河流均有分布，且都保持了较高的均匀度（E≥0.9298），亚型1中分布于长江水体的基因群落具有明显高于其他河流和平均值的多样性（H’ = 2.4774）和物种优势度（D = 0.9107），而亚型2、3的多样性和优势度最高值则均出现在黄河水体中；松花江与海河之间的群落相似性最高，达到0.806 2，而黄河与珠江基因群落之间的相异性则达到了0.580 0. 说明在调查中采样的6条河流中均分布着结构稳定且基因优势突出的CTX-M型耐药基因，已对水体生态安全构成了隐患，需要结合进一步研究对其进行防治，以避免耐药基因以质粒为媒介向其他微生物传播造成抗生素耐药性扩散.
Abstract:
In order to understand the distribution of CTX-M type resistance genes and their variation diversity from the typical city rivers, so as to avoid the generation and dissemination of antibiotic resistance, we investigated six rivers in China. Water samples were collected from six typical rivers in eastern China, then metagenomics idea combined with restriction fragment length polymorphism (RFLP) technology were employed for PCR products assay. All data were processed by ecological statistics. All of six surveyed rivers were distributed with the three subtypes of CTX-M genes with a high evenness (E ≥ 0.9298). For genotype 1, the gene communities from the Yangtze River had a significantly higher diversity (H’ = 2.4774) and species dominance (D = 0.9107) than in other rivers and the average, while the highest diversity and dominance value of genotype 2 and 3 appeared in the waters of the Yellow River. The gene community similarity between the Sungari and the Haihe was the highest，reaching 0.806 2, while the dissimilarity between the gene communities in the Yellow River and the Pearl River reached 0.580 0. The CTX-M resistance gene communities and plasmid metagenomic showed stable structures as well as prominent dominances in six sampled rivers, which is a threat to ecological safety of water bodies. Further researches are needed for controlling and avoiding the spread of resistant genes to other micro-organisms mediated by plasmid and causing diffusion of antibiotic resistance.

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备注/Memo

备注/Memo:
国家自然科学基金面上项目（81372947）和青年基金项目（81202163）资助
更新日期/Last Update: 2014-03-06