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[1]王明跃,张文学,王海英,等.不同窖龄窖泥古菌的系统发育多样性分析[J].应用与环境生物学报,2012,18(06):1043-1048.[doi:10.3724/SP.J.1145.2012.01043]
 WANG Mingyue,ZHANG Wenxue,WANG Haiying,et al.Phylogenetic Diversity Analysis of Archaeal in the Pit Mud with Different Cellar Age[J].Chinese Journal of Applied & Environmental Biology,2012,18(06):1043-1048.[doi:10.3724/SP.J.1145.2012.01043]
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不同窖龄窖泥古菌的系统发育多样性分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年06期
页码:
1043-1048
栏目:
研究简报
出版日期:
2012-12-25

文章信息/Info

Title:
Phylogenetic Diversity Analysis of Archaeal in the Pit Mud with Different Cellar Age
作者:
王明跃 张文学 王海英 刘超兰
(1阜阳职业技术学院 阜阳 236031)
(2四川大学轻纺与食品学院 成都 610065)
Author(s):
WANG Mingyue ZHANG Wenxue WANG Haiying LIU Chaolan
(1Fuyang Vocational and Technical College, Fuyang 236031, Anhui, China)
(2College of Light Industry & Textile & Food, Sichuan University, Chengdu 610065, China)
关键词:
窖泥古菌类群16S rDNA序列克隆文库系统发育多样性
Keywords:
pit mud archaeal community 16S rDNA sequence clone library phylogenetic diversity
分类号:
TS261.1
DOI:
10.3724/SP.J.1145.2012.01043
文献标志码:
A
摘要:
为探索不同窖龄窖泥古菌类群与演替规律,分别提取20、50和150年窖龄窖泥样品总DNA,应用PCR-ARDRA和16S rDNA克隆测序技术对不同窖龄窖泥古菌群落结构进行研究. 结果表明:191个克隆子分成22个可操作分类单元(OTUs),系统发育分析归为6个类群,分别属于甲烷八叠球菌属(Methanosarcina)、甲烷杆菌属(Methanobacterium)、甲烷袋状菌属(Methanoculleus)、甲烷粒菌属(Methanocorpusculum)、甲烷鬃菌属(Methanosaeta)和甲烷螺菌属(Methanospirillum). 不同窖龄窖泥的优势古菌类群有明显差异,20年窖泥优势古菌类群以乙酸利用型Methanosarcina、Methanosaeta为主,50年、150年龄窖泥优势古菌类群以氢气营养型Methanocorpusculum、Methanobacterium为主;随着窖龄的增加,窖泥古菌Shannon-Wiener多样性指数(H)和Berger-Parker优势度指数(D)呈增加的趋势,Pielou均匀度指数(J)呈降低趋势;不同窖龄窖池古菌的相似性系数为0.429~0.677,50年和150年窖泥的菌群结构最为相似. 本研究揭示了不同窖龄窖泥古菌系统发育多样性的差异与窖泥老熟的关系. 图1 表1 参22
Abstract:
In order to investigate the clusters and succession rules of archaeal in the pit mud, the total DNAs were extracted from the pit mud with cellar age of 20, 50 and 150 years, respectively. The community structure was studied by polymerase chain reaction-amplified rDNA restriction analysis (PCR-ARDRA) and sequencing of 16S rDNA. The results showed that 191 clones from the 16S rDNA library fell into 22 operational taxonomic units (OTUs) from 6 groups including Methanosarcina, Methanobacterium, Methanoculleus, Methanocorpusculum, Methanosaeta and Methanospirillum. Diversity was obvious in the predominant archaeal from the pit mud with different cellar age. The predominant archaeal from the pit mud with cellar age of 20 years were Methanosarcina and Methanosaeta, which produced methane by utilizing acetic acid. However, the predominant archaeal from the pit mud with cellar age of 50 and 150 years were Methanocorpusculum and Methanobacterium, which utilizing hydrogen. With the increasing cellar age, the Shannon-Wiener index (H) and Berger-Parker index (D) of archaeal in the pit mud showed an increasing trend, while the Pielou index (J) showed a decreasing trend. The Sorenson similarity index of the pit mud with different cellar age was the range of 0.429~0.677, and there was the highest similarity of community structure between the pit mud with cellar age of 50 and 150 years. This study revealed the relationship between archaeal phylogenetic diversity of pit mud and the aging of pit mud. Fig 1, Tab 1, Ref 22

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备注/Memo

备注/Memo:
国家自然科学基金面上项目(No. 31071585)和安徽省高校省级自然科学研究项目(No. KJ2012B140)资助 Supported by the National Natural Science Foundation of China (No. 31071585) and the University Provincial Natural Science Research Project of Anhui, China (No. KJ2012B140)
更新日期/Last Update: 2012-12-28