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[1]龙飞,靳艳玲,赵云,等.丙酮丁醇梭菌Clostridium acetobutylicum CICC 8012发酵鲜芭蕉芋生产丁醇[J].应用与环境生物学报,2013,19(01):54-60.[doi:10.3724/SP.J.1145.2013.00054]
 LONG Fei,JIN Yanling,ZHAO Yun,et al.Optimization of Butanol Production from Canna edulis Ker by Clostridium acetobutylicum Using Response Surface Methodology[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):54-60.[doi:10.3724/SP.J.1145.2013.00054]
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丙酮丁醇梭菌Clostridium acetobutylicum CICC 8012发酵鲜芭蕉芋生产丁醇()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年01期
页码:
54-60
栏目:
研究论文
出版日期:
2013-02-25

文章信息/Info

Title:
Optimization of Butanol Production from Canna edulis Ker by Clostridium acetobutylicum Using Response Surface Methodology
作者:
龙飞靳艳玲赵云郜晓峰李宇浩陈谦赵海
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院大学 北京 100049)
(3中国科学院环境与应用微生物重点实验室 成都 610041)
(4四川大学生命科学学院 成都 610064)
Author(s):
LONG FeiJIN YanlingZHAO YunGAO XiaofengLI YuhaoCHEN QianZHAO Hai
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China )
(2University of Chinese Academy of Sciences, Beijing 100049, China)
(3Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China )
(4College of Life Sciences, Sichuan University, Chengdu 610064, China)
关键词:
丁醇鲜芭蕉芋丙酮丁醇梭菌响应面法发酵
Keywords:
butanol raw Canna edulis Ker Clostridium acetobutylicum response surface methodology fermentation
分类号:
TQ923 : S216
DOI:
10.3724/SP.J.1145.2013.00054
文献标志码:
A
摘要:
以非粮作物鲜芭蕉芋为原料,利用丙酮丁醇梭菌CICC 8012发酵生产丁醇. 采用中心组合实验设计(CCD),选取初始糖浓度、接种量、中性红、乙酸铵为主要影响因素,对发酵条件进行优化,建立以丁醇产量为响应值的数学模型. 对模型求解得到:初始糖浓度62.25 g/L,接种量为10.81%,中性红为0.81 g/L,乙酸铵为0.574 g/L时,最终的丁醇产量为12.83 g/L. 验证实验结果表明,在优化条件下丁醇发酵产量达到 12.73 g/L,证明了模型可靠有效.
Abstract:
Raw Canna edulis Ker, one of the non-food crops, was utilized in this study to produce butanol by Clostridium acetobutylicum CICC 8012. Central Composite Design (CCD) was adopted to optimize experiment conditions including four crucial factors which were initial sugar concentration, inoculation volume, neutral red and ammonium acetate. According to the results of the model, the optimal conditions of butanol fermentation were that: initial sugar concentration 62.25 g/L, inoculation volume 10.81%, neutral red usage 0.81 g/L, ammonium acetate usage 0.574 g/L and final butanol yield of 12.83 g/L. In the verification experiment, the butanol yield of 12.73 g/L was obtained under the optimal conditions, which indicated that the model was reliable and effective.

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备注/Memo

备注/Memo:
国家科技支撑计划项目(2011BAD22B03)、中国科学院知识创新工程重要方向项目(2011BAD22B03,KSCX2-EW-G-1-1)和现代农业产业技术体系项目(CARS-11-A-04)资助 Supported by the National Sci-tech Pillar R & D Program of China (No. 2011BAD22B03), the Major Projects of Knowledge Innovation Program of Chinese Academy of Sciences (Nos. 2011BAD22B03, KSCX2-EW-J-22), and the China Agriculture Research System Project (No. CARS-11-A-04)
更新日期/Last Update: 2013-02-26