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[1]韩永光,骆玥,魏徵霄,等.拟南芥蛋白磷酸酶PPH1的结构预测与功能分析[J].应用与环境生物学报,2013,19(01):69-73.[doi:10.3724/SP.J.1145.2013.00069]
 HAN Yongguang,LUO Yue,WEI Zhengxiao,et al.Structure Prediction and Function Analysis of Protein Phosphatase PPH1 from Arabidopsis thaliana[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):69-73.[doi:10.3724/SP.J.1145.2013.00069]
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拟南芥蛋白磷酸酶PPH1的结构预测与功能分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年01期
页码:
69-73
栏目:
研究论文
出版日期:
2013-02-25

文章信息/Info

Title:
Structure Prediction and Function Analysis of Protein Phosphatase PPH1 from Arabidopsis thaliana
作者:
韩永光骆玥魏徵霄唐秋菊杜林方
(四川大学生命科学学院,生物资源与生态环境教育部重点实验室 成都 610064)
Author(s):
HAN YongguangLUO YueWEI ZhengxiaoTANG QiujuDU Linfang
(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
关键词:
PPH1去磷酸化PP2C核心结构域抗体
Keywords:
PPH1 dephosphorylation PP2C core domain antibody
分类号:
Q946.5
DOI:
10.3724/SP.J.1145.2013.00069
文献标志码:
A
摘要:
拟南芥PPH1属于蛋白磷酸酶2C(PP2C)家族,主要参与捕光色素复合物LHCⅡ蛋白去磷酸化调控与光系统的状态转移,与STN7蛋白激酶共同参与调控LHCⅡ的磷酸化与去磷酸化. 为了解此酶的具体的催化机制和空间结构,使用生物信息学的方法对PPH1的疏水性、跨膜区、膜体和二级结构进行分析,构建系统进化树并通过同源建模的方法建立其核心结构域的三级结构,围绕蛋白磷酸酶的结构和可能的作用机制关系进行探讨. 在以上分析的基础上选择特异位点合成多肽后,免疫家兔并成功制备抗体. 蛋白印迹结果显示制备得到了PPH1特异抗体,证实此磷酸酶结构得以正确地预测. 研究为今后进一步研究其结构和功能以及突变体的设计提供了理论基础.
Abstract:
PPH1, a member of protein phosphatase 2C (PP2C) of Arabidopsis thaliana, is involved in the dephosphorylation of LHCII and the state transitions. The phosphorylation and dephosphorylation of LHCII appear to be specifically mediated by the kinase/phosphatase pair STN7 and PPH1. However, the catalytic mechanism and structure of PPH1 remain uncertain at present. In this research, the hydrophobic property, transmembrane domain, motif and secondary structure of PPH1 were discussed using a systematical bioinformatics method and a phylogenetic tree was constructed. Three dimensional structures of core domains of PPH1 were predicted by using homologous modeling method. On the basis of the above analysis, one anti-peptide antibody was designed. Western blot analysis with thylakoid membranes of A. thaliana showed the polyclonal antibodies against the peptides were able to act with the parent protein. Therefore, structure prediction of the PPH1 provided the foundation for further studying the functions and designing mutant of PPH.

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备注/Memo

备注/Memo:
国家自然科学基金项目(30870181)和国家重点基础研究发展计划项目资助 Supported by the National Natural Science Foundation of China (No. 30870181) and the National Basic Research “973” Program of China (No. 2009CB118502)
更新日期/Last Update: 2013-02-26