|本期目录/Table of Contents|

[1]柳芳,田伟,李凌之,等.生防枯草芽孢杆菌SQR9固体发酵生产生物有机肥的工艺优化[J].应用与环境生物学报,2013,19(01):90-95.[doi:10.3724/SP.J.1145.2013.00090]
 LIU Fang,TIAN Wei,LI Lingzhi,et al.Optimization of Solid-state Fermentation Conditions for Antagonistic Bacillus subtilis SQR9 Producing Bio-organic Fertilizer[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):90-95.[doi:10.3724/SP.J.1145.2013.00090]
点击复制

生防枯草芽孢杆菌SQR9固体发酵生产生物有机肥的工艺优化()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
19卷
期数:
2013年01期
页码:
90-95
栏目:
研究论文
出版日期:
2013-02-25

文章信息/Info

Title:
Optimization of Solid-state Fermentation Conditions for Antagonistic Bacillus subtilis SQR9 Producing Bio-organic Fertilizer
作者:
柳芳田伟李凌之杨兴明沈标沈其荣
(南京农业大学江苏省固体有机废弃物资源化高技术研究重点实验室 南京 210095)
Author(s):
LIU FangTIAN WeiLI LingzhiYANG XingmingSHEN BiaoSHEN Qirong
(Jiangsu Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
枯草芽孢杆菌固体发酵发酵工艺优化生物有机肥
Keywords:
Bacillus subtilis solid-state fermentation fermentation condition optimization bio-organic fertilizer
分类号:
S144.2
DOI:
10.3724/SP.J.1145.2013.00090
文献标志码:
A
摘要:
枯草芽孢杆菌SQR9是一株广谱性的拮抗菌,为促进农业废弃物的资源化利用以及植物土传病害的生物防治,分别以氨基酸有机肥、牛粪堆肥、猪粪堆肥、中药渣堆肥为原料,研究利用SQR9进行二次固体发酵生产微生物有机肥的最佳工艺条件及其原料最佳配比. 结果表明,SQR9固体发酵的最佳参数为:发酵最佳原料为中药渣堆肥,接种量10%(φ/mL g-1),麸皮添加量15%,初始含水量65%,发酵温度37 ℃,翻抛次数1次/24 h,在此发酵条件下,SQR9菌株的活菌总量可达到1.20×1011 CFU g-1. 中药渣堆肥︰牛粪堆肥︰猪粪堆肥︰氨基酸有机肥的最佳配比为为40︰6︰0︰8,在此原料配比和最佳发酵条件下,SQR9活菌总量可达1.97×1010 CFU g-1. 因此,用农业废弃物采用合适的工艺条件可以生产出高质量的生物有机肥.
Abstract:
Bacillus subtilis SQR9 is an antagonist with a very broad antagonistic spectrum to soil borne pathogens. Production of biofertilizer containing SQR9 using agricultural waste is very important to reuse agricultural waste as sources and biocontrol soil borne plant diseases. In the present study, amino acid organic fertilizer, pig slurry compost, cattle manure compost and Chinese medicinal herb residue compost were used as ferment materials to investigate their optimal ratio and optimal fermenting conditions for SQR9 to produce bio-organic fertilizer. The optimum parameters were obtained as follows: Chinese medicinal herb residue compost as principal raw material with 10% of inoculation, 15% of wheat bran supplementation, 65% of initial moisture content, fermentation temperature 37 ℃, and once a day of turning over. Under the optimized conditions, the bacterial number of SQR9 could reach to 1.20×1011 CFU g-1. The optimum proportion of four organic composts was studied by orthogonal test. The optimum proportion obtained was that Chinese medicinal herb residue compost : cattle manure compost : pig slurry organic fertilizer : amino acid organic fertilizer was 40 : 6 : 0 : 8, in which the bacterial number of SQR9 could be as high as 1.97×1010 CFU g-1. This research therefore suggested that high quality biofertilizer could be produced using certain agricultural waste under suitable condition.

参考文献/References:

1 张树生. 微生物有机肥缓解黄瓜枯萎病的生物学效应及其作用机制[D]. 南京: 南京农业大学, 2009. 9 [Zhang SS. Control of Fusarium wilt disease of cucumber plants with the application of a bio-organic fertilizer and its mechanism [D]. Nanjing: Nanjing Agricultural University, 2009. 9]
2 孙思, 韦爱梅, 伍慧雄, 王军. 青枯病的化学与生物防治研究进展[J]. 江西植保, 2004, 27 (4): 157-162 [Sun S, Wei AM, Wu HX, Wang J. Advances in research on chemical and biological control of plant bacterial wilt [J]. Jiangxi Plant Prot, 2004, 27 (4): 157-162]
3 周凤珍. 黄瓜枯萎病病原菌鉴定及其苗期抗病鉴定[J]. 植物保护, 1985, 5: 16-17
4 Handelsman J, Raffel S, Mester EH. Biological control of damping-off of alfalfa seedlings with Baeillus cereus UW85 [J]. Appl Environ Microbiol, 1990, 56: 713-718
5 Handelsman J, Stabb EV. Biocontrol of soil-borne plant pathogens [J]. Plant Cell, 1996, 8 (10): 1855-1869
6 Weller DM. Biological control of soil-borne plant pathogens in the rhizosphere with bacteria [J]. Annu Rev Phytopathol, 1988, 26: 397-407
7 赵小蓉, 林启美. 微生物解磷的研究进展[J]. 土壤肥料, 2001 (7): 7-11 [Zhao XR, Lin QM. A review of phosphate dissolving microorganisms [J]. Soils & Fert, 2001 (7): 7-11]
8 周莉华, 李维炯, 倪永珍. 长期施用EM生物有机肥对冬小麦生产的影响[J]. 农业工程学报, 2005, 21: 221-224 [Zhou LH, Li WJ, Ni YZ. Effects of long-term application of EM biological-organic fertilizer on winter wheat production [J]. Trans CSAE, 2005, 21: 221-224]
9 王琦. 牛粪发酵生产生物有机肥的工艺优化及应用研究[D]. 西安: 西北大学, 2008 [Wang Q. Optimization of cattle manure fermentation and its application [D]. Xi’an: Northwest University, 2008]
10 肖相政, 刘可星, 廖宗文. 生物有机肥对番茄青枯病的防效研究及机理初探[J]. 农业环境科学学报, 2009, 28 (11): 2368-2373 [Xiao XZ, Liu KX, Liao ZW. Disease-control effect and mechanism research of biological organic fertilizer on tomato bacterial wilt [J]. J Agro-Environ Sci, 2009, 28 (11): 2368-2373]
11 凌宁, 王秋君, 杨兴明, 徐阳春, 黄启为, 沈其荣. 根际施用微生物有机肥防治连作西瓜枯萎病研究[J]. 植物营养与肥料学报, 2009, 15 (5): 1136-1141 [Ling N, Wang QJ, Yang XM, Xu YC, Huang QW, Shen QR. Control of Fusarium wilt of watermelon by nursery application of bio-orgnic fertilizer [J]. Plant Nutr & Fert Sci, 2009, 15 (5): 1136-1141]
12 何欣, 郝文雅, 杨兴明, 沈其荣, 黄启为. 生物有机肥对香蕉植株生长和香蕉枯萎病防治的研究[J]. 植物营养与肥料学报, 2010, 16 (4): 978-985 [He X, Hao WY, Yang XM, Shen QR, Huang QW. Effects of bioorganic fertilization on growth and controlling Fusarium-wilt disease of banana [J]. Plant Nutr & Fert Sci, 2010, 16 (4): 978-985]
13 周新根, 朱宗源, 汪树俊. 辅以拮抗微生物的有机添加物对蔬菜土传病原菌的生物防治作用[J]. 上海农业学报, 1994, 10 (4): 53-58 [Zhou XG, Zhu ZY, Wang SJ. Effect of organic amendment coated with antigonistic microorganisms on biological control for soil borne pathogens on vegetables [J]. Acta Agric Shanghai, 1994, 10 (4): 53-58]
14 常志州, 马艳, 黄红英, 叶小梅, 张建英. 辅以拮抗菌的有机肥对辣椒疫病生防效果的研究[J]. 土壤肥料, 2005 (2): 28-30 [Chang ZZ, Ma Y, Huang HY, Ye XM, Zhang JY. Efficacy of compost riched with fungi F-310 against Phytophthora capsici. Leonnian in pepper [J]. Soils & Fert, 2005 (2): 28-30]
15 胡艳霞, 孙振钧, 王东辉, 孙永明. 蚯蚓粪中拮抗微生物分析[J]. 应用与环境生物学报, 2004, 10 (1): 99-103 [Hu YX, Sun ZJ, Wang DH, Sun YM. Anlalysis of antagomistic microorganism in vermicompost [J]. Chin J Appl Environ Biol, 2004, 10 (1): 99-103]
16 陈谦, 张新雄, 赵海, 官家发. 生物有机肥中几种功能微生物的研究及应用概况[J]. 应用与环境生物学报, 2010, 16 (2): 294-300 [Chen Q, Zhang XX, Zhao H, Guan JF. Advance in research and application of some functional microbes in bio-organic fertilizer [J]. Chin J Appl Environ Biol, 2010, 16 (2): 294-300]
17 董峻. 我国在利用有机肥防治作物土传病害方面取得新成就[J]. 北京农业, 2010 (12): 22
18 Cao Y, Zhang ZH, Ling N, Yuan YJ, Shen B, Shen QR. Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots [J]. Biol Fert Soils, 2011, 47 (5): 495-506
19 张志焱, 徐海燕, 杨军方. 枯草芽孢杆菌固体发酵培养基的优化[J]. 饲料博览, 2005 (9): 34-36 [Zhang ZY, Xu HY, Yang JF. Optimization of solid fermetation media for B. subtilis [J]. Feed Rev, 2005 (9): 34-36]
20 王扬, 张志毅, 杨胜利. par区域的克隆及其对质粒pUC9稳定性的影响[J]. 生物工程学报, 1992, 8 (2): 134-139 [Wang Y, Zhang ZY, Yang SL. Cloning of Par region and its effect on stability of plasmid pUC9 [J]. Chin J Biotechnol , 1992, 8 (2): 134-139]
21 张秋霞, 钟增涛, 徐阳春, 沈其荣. 多粘芽孢杆菌芽孢荧光原位杂交检测技术研究及其在有机肥发酵中的应用[J]. 植物营养与肥料学报, 2010, 16 (5): 1276-1281 [Zhang QX, Zhong ZT, Xu YC, Shen QR. Detection of Paenibacillus polymyxa spores by fluorescent in situ hybridization (FISH) and its application in organic compost fermentation [J]. Plant Nutr & Fert Sci, 2010, 16 (5): 1276-1281]
22 王永红, 冉炜, 张国富, 杨兴明, 徐阳春, 沈其荣. 混合菌种固体发酵菜粕生产氨基酸肥料的条件研究[J]. 中国农业科学, 2009, 42 (10): 3530-3540 [Wang YH, Ran W, Zhang GF, Yang XM, Xu YC, Shen QR. Study on conditions in solid-state fermentation of rapeseed meal by mixed strains for amino acid fertilizer [J]. Sci Agric Sin, 2009, 42 (10): 3530-3540]
23 贾小红, 黄元仿. 有机肥料加工与施用[M]. 2版. 北京: 化学工业出版社, 2010: 241
24 李阜棣, 喻子牛, 何绍江. 农业微生物学实验技术[M]. 北京: 中国农业出版社, 1996: 178-181
25 陶玉贵. 生物农药固态发酵条件的研究[J]. 安徽师范大学学报, 2001, 24 (1): 31-34 [Tao YG. A study of solid-state fermentation conditions for producing biological pesticide [J]. J Anhui Norm Univ, 2001, 24 (1): 31-34]
26 尹光琳, 战立克, 赵根楠. 发酵工业全书[M]. 北京: 中国医药科技出版社, 1992: 216
27 钱晓雍, 沈根祥, 黄丽华, 奚刚. 畜禽粪便堆肥腐熟度评价指标体系研究[J]. 农业环境科学学报, 2009, 28 (3): 549-554 [Qian XY, Shen GX, Huang LH, Xi G. An index system for evaluating the maturity of animal manure composting [J]. J Agro-Environ Sci, 2009, 28 (3): 549-554]
28 章四平, 刘圣明, 王建新, 周明国. 枯草芽孢杆菌生防菌株NJ18的发酵条件优化[J]. 南京农业大学学报, 2010, 33 (2): 58-62 [Zhang SP, Liu SM, Wang JX, Zhou MG. Optimizing fermentation condition for the antagonistic Bacillus subtilis NJ18 strain [J]. J Nanjing Agric Univ, 2010, 33 (2): 58-62]
29 应俊辉. 影响污泥高温好氧堆肥的主要因素及其控制[J]. 丽水学院学报, 2006, 28 (5): 59-61 [Ying JH. A discussion on influence factors and control of thermophilic aerobic composting of sludge [J]. J Lishui Univ, 2006, 28 (5): 59-61]
30 李巧如, 任健康, 刘宗智, 赵院利. 18种中草药抗菌作用的筛选[J]. 陕西中医, 2002, 23 (6): 555
31 吕黄珍, 韩鲁佳, 杨增玲. 猪粪麦秸反应器好氧堆肥工艺参数优化[J]. 农业机械学报, 2008, 39 (3): 101-105 [Lü HZ, Han LJ, Yang ZL. Optimization of aerobic composting parameters on pig slurry-wheat straw reactor [J]. Trans Chin Soc Agric Mach, 2008, 39 (3): 101-105]
32 Jackson AM, Whipps JM, Lynch JM. Effects of temperature, pH and water potential on growth of the four fungi with disease biocontrol potential [J]. World Microbiol Biotechnol, 1991, 7 (2): 494-501
33 Kundsen GR, LI B. Effects of temperature, soil moisture, and wheat brab on growth of trichoderma harzianum from the alginate pellets [J]. Phytopathology, 1990, 90 (1): 724-727

相似文献/References:

[1]邓宇,华兆哲,** 赵志军,等.氮源对枯草芽孢杆菌WSHDZ-01合成过氧化氢酶的影响[J].应用与环境生物学报,2008,14(04):544.
[2]郭英,刘栋,赵蕾.生防枯草芽孢杆菌胞外植酸酶对小麦耐盐性的影响[J].应用与环境生物学报,2009,15(01):39.[doi:10.3724/SP.J.1145.2009.00039]
 GUO Ying,LIU Dong,ZHAO Lei.Effect of Extracellular Phytase Produced by Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):39.[doi:10.3724/SP.J.1145.2009.00039]
[3]付雯,张晓勇,周金燕,等.固定化枯草芽孢杆菌发酵生产捷安肽素[J].应用与环境生物学报,2009,15(02):230.[doi:10.3724/SP.J.1145.2009.00230]
 FU Wen,ZHANG Xiaoyong,et al.Jiean-peptide Prodution by Immobilized Cell Fermentation of Bacillus subtilis[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):230.[doi:10.3724/SP.J.1145.2009.00230]
[4]刘项羽,徐美娟,杨套伟,等.β-甘露聚糖酶基因在枯草芽孢杆菌中的克隆及表达[J].应用与环境生物学报,2012,18(04):672.[doi:10.3724/SP.J.1145.2012.00672]
 LIU Xiangyu,XU Meijuan,YANG taowei,et al.Cloning and Expression of β-mannanase Gene in Bacillus subtilis[J].Chinese Journal of Applied & Environmental Biology,2012,18(01):672.[doi:10.3724/SP.J.1145.2012.00672]
[5]包兴艳,郝建华,陈世建,等.产酯酶B1海洋枯草芽孢杆菌C5发酵条件优化[J].应用与环境生物学报,2012,18(06):999.[doi:10.3724/SP.J.1145.2012.00999]
 BAO Xingyan,HAO Jianhua,CHEN Shijian,et al.Optimization of Fermentation Conditions for Marine Bacillus subtilis C5 Producing Esterase B1[J].Chinese Journal of Applied & Environmental Biology,2012,18(01):999.[doi:10.3724/SP.J.1145.2012.00999]
[6]邹艳玲,徐美娟,饶志明.耐热β-淀粉酶高产菌株的筛选及其产酶条件优化[J].应用与环境生物学报,2013,19(05):838.[doi:10.3724/SP.J.1145.2013.00838]
 ZOU Yanling,XU Meijuan,RAO Zhiming.Screening and Fermentation Optimization for a High-yield Thermostable -Amylase Producing Strain[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):838.[doi:10.3724/SP.J.1145.2013.00838]
[7]王远,高秋强,辛秀娟,等.β-葡萄糖苷酶基因和内切葡聚糖酶基因在枯草芽孢杆菌中的表达[J].应用与环境生物学报,2013,19(06):990.[doi:10.3724/SP.J.1145.2013.00990]
 WANG Yuan,GAO Qiuqiang,XIN Xiujuan,et al.Expression of Endoglucanase Gene and β-Glucosidase Genes in Bacillus subtilis[J].Chinese Journal of Applied & Environmental Biology,2013,19(01):990.[doi:10.3724/SP.J.1145.2013.00990]
[8]程毅鹏,饶志明,杨套伟,等.一种新型抗性质粒的构建及其在核黄素生产菌中的运用[J].应用与环境生物学报,2015,21(03):435.[doi:10.3724/SP.J.1145.2014.12003]
 CHENG Yipeng,RAO Zhiming,YANG Taowei,et al.Construction of a new resistance plasmid capable of riboflavin production in Bacillus subtilis RF1[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):435.[doi:10.3724/SP.J.1145.2014.12003]
[9]高志强,李茜,徐岳松,等.高产木聚糖酶细菌的筛选、鉴定及其部分酶学特性[J].应用与环境生物学报,2017,23(03):443.[doi:2014.05011]
 GAO Zhiqiang,LI Xi,XU Yuesong,et al.Isolation and identification of a xylanase-producing bacterium and its enzymatic characteristics[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):443.[doi:2014.05011]
[10]周强,王淳,李云萍,等.黄酮醇类化合物的合成与抗菌活性[J].应用与环境生物学报,2017,23(2):232.[doi:10.3724/SP.J.1145.2016.04019]
 ZHOU Qiang,WANG Chun**,et al.Synthesis and antibacterial activity of flavonols[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):232.[doi:10.3724/SP.J.1145.2016.04019]

备注/Memo

备注/Memo:
江苏省和科技部科技成果转化资金项目(BA2008027,2009120)资助 Supported by the Fund Project for Science and Technology Achievement Transformation of Jiangsu Province (No. BA2008027) and the Ministry of Science and Technology of China (No. 2009120)
更新日期/Last Update: 2013-02-26