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[1]王鹏,姜勇,田敬云,等.多拷贝表达转鲑鱼降钙素基因酵母的急性、亚急性毒性[J].应用与环境生物学报,2012,18(04):630-634.[doi:10.3724/SP.J.1145.2012.00630]
 WANG Peng,JIANG Yong,TIAN Jingyun,et al.Acute and Subacute Toxicity of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):630-634.[doi:10.3724/SP.J.1145.2012.00630]
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多拷贝表达转鲑鱼降钙素基因酵母的急性、亚急性毒性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年04期
页码:
630-634
栏目:
研究论文
出版日期:
2012-08-25

文章信息/Info

Title:
Acute and Subacute Toxicity of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin
作者:
王鹏姜勇田敬云马健张学成路兴波
(1山东省农业科学院 济南 250100)
(2 青岛国家海洋科学研究中心 青岛 266071)
(3中国海洋大学 青岛 266071)
Author(s):
WANG PengJIANG YongTIAN JingyunMA JianZHANG XuechengLU Xingbo
(1Shandong Academy of Agricultural Sciences, Ji’nan 250100, China)
(2National Oceanographic center, Qingdao 266071, China)
(3Ocean University of China, Qingdao 266071, China)
关键词:
转鲑鱼降钙素酵母小鼠大鼠急性毒性亚急性毒性
Keywords:
recombinant Saccharomyces cerevisiae expressing salmon calcitonin KM mouse Wistar rats acute toxicity subacute toxicity
分类号:
R965.3 : R282.74
DOI:
10.3724/SP.J.1145.2012.00630
文献标志码:
A
摘要:
口服转鲑鱼降钙素基因酵母为鲑鱼降钙素的应用开辟了一条新的途径. 为评价转鲑鱼降钙素基因酵母的安全性,将转基因酵母分别灌胃昆明种小鼠和Wistar大鼠进行急性毒性实验(7 d)、亚急性毒性实验(8周). 急性毒性实验结果表明灌喂转基因酵母对小鼠无致死效应(LD50 > 10 000 mg/kg);亚急性毒性实验中高(2.0 g/kg)、低(0.5 g/kg)剂量组动物的一般状况、体重、主要脏器系数、血液学指标及血液生化指标与各对照组比较,均未见明显差异(P > 0.05),组织病理切片检查未发现病理性变化. 实验结果说明转鲑鱼降钙素基因酵母不会对实验动物造成不良影响,是安全无毒的. 图3 表3 参16
Abstract:
Oral administration recombinant Saccharomyces cerevisiae expressing salmon calcitonin made a new method for salmon calcitonin delivery system. Transgenic S. cerevisiae was evaluated for its acute (7 days) and subacute toxicity (8 weeks) by the oral route in KM mouse and Wistar rats respectively. In the acute toxicity test, every group did not produce any hazardous symptoms or deaths (LD50 > 10 000 mg/kg). The subacute treatment with transgenic S. cerevisiae (2.0 and 0.5 g/kg) failed to change body weight gain, food and water consumption. Organ/weight ratios, haematological and biochemical indices analysis showed no significant differences (P > 0.05) in any of the parameters examined. There was no pathological change observed in slice. These results suggested that recombinant S. cerevisiae expressing salmon calcitonin had no negative influences on the tested animal. It was safe and nontoxic. Fig 3, Tab 3, Ref 16

参考文献/References:

Kraenzlin ME, Seibel MJ, Trechsel U, Boerlin V, Azria M, Kraenzlin CA, Haas HG. The effect of intranasal Salmon calcitonin on postmenopausal bone turnover as assessed by biochemical markers: Evidence of maximal effect after 8 weeks of continuous treatment. Calcified Tissue Intern, 1996, 58 (4): 216~220.
Cheng WP, Thompson C, Ryan M, Aguirre M, Tetley L, David JB. In vitro and in vivo characterisation of a novel peptide delivery system: Amphiphilic polyelectrolyte-salmoncalcitonin nanocomplexes. J Controlled Release, 2010, 147 (2): 393~405
Lynn A, Hunter H. Calcitonin: Physiology and pathophysiology. N England J Med, 1981, 304 (5): 269~276
Guerra S, Vega P, Velázquez-del V, Hernández L. Antifungal activity and release of compounds on Rhizopus stolonifer Vuill. by effect of chitosan with different molecular weights. Pestic Biochem & Physiol, 2009, 93 (1): 18~22
Yu SY, Ju YJ, Seung HO, Sun DY, Kang ChL. Improved intestinal delivery of salmon calcitonin by Lys18-amine specific PEGylation: Stability, permeability, pharmacokinetic behavior and in vivo hypocalcemic efficacy. J Controlled Release, 2006, 114: 334~342
Yang M, Zhang JX, Tian S. Construction of engineered saccharomyces cerevisiae for steadily metabolizing glucose and xylose to ethanol. Chin J Appl & Environ Biol (应用与环境生物学报), 2009, 15 (2): 258~261
Stéphanie B, Richard A, Laurent L, Sylvain D, Sylvie MB, Monique A. Living recombinant Saccharomyces cerevisiae secreting proteins or peptides as a new drug delivery in the gut. J Biotechnology, 2004, 110: 37~49
Huang BW, Lin XD, Su FR, Guan X. Correlation of enterotoxin gene and plcR gene in Bacillus thuringiensis and toxicity of enterotoxin to mice. Chin J Appl Eenviron Biol (应用与环境生物学报), 2011, 17 (3): 418~421
Fukawa K, Kawano O, Hibi M, Misaki N, Ohba S, Hatanaka Y. A method for evaluating analgesic agents in rats. J Pharmacol Methods, 1980, 4 (3): 251~259
Luis ES, Analía EC, Marcos RF, Martin FR, Horacio ER, Estela MM. A standardized surgical technique for rat superior cervical ganglionectomy. J Neuroscience Methods, 2010, 192 (1): 22~33
Shayne CG. Drug Safety Evaluation. Beijing: Chemical Industry Press, 2006. 106~140
Gerald L, Kennedy JR, Rayanne LF, Bruce AB. Estimation of acute oral toxicity in rats by determination of the approximate lethal dose rather than the LD50. J Appl Toxicol, 1986, 6: 145~148
Yang DK, Jin FS, Hu W, Zhang Y, Jiang J. Recombination and expression of Mycobacterium tuberculosis Ag85B/IL-2-GST fusion protein in E. coli. Immunol J (免疫学杂志), 2006, 22 (3): 350~351
Seiya K, Tatsuki S, Muneo F, Tadayoshi O, Shunji H, Takashi S. Enhancement of nasal salmon calcitonin absorption by lauroylcarnitine chloride in rats. Pharmaceutical Res, 1996, 13: 739~743
Latha M, Geentha T, Varalakshmi P. Effect of Vernonia cinerea less flower extract in adjuvant induced arthritis. Gen Pharmacol: Vasc Syst, 1998, 31 (4): 601~606
Kaneko J, Harvey J, Bruss M. Clinical Biochemistry of Domestic Animals. San Diego: Academic Press, 2008

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备注/Memo

备注/Memo:
国家“863”高技术发展与研究计划项目(No. 819-04-10)资助
更新日期/Last Update: 2012-08-21