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[1]周婷,袁世斌,邹晓莉.一株踝节霉菌的培养特性及其在污泥生物淋滤中的应用[J].应用与环境生物学报,2012,18(01):128-133.[doi:10.3724/SP.J.1145.2012.00128]
 ZHOU Ting,YUAN Shibin,ZOU Xiaoli.Cultural Characteristics of a Strain of Talaromyces sp. and Its Application in Bioleaching of Sewage Sludge[J].Chinese Journal of Applied & Environmental Biology,2012,18(01):128-133.[doi:10.3724/SP.J.1145.2012.00128]
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一株踝节霉菌的培养特性及其在污泥生物淋滤中的应用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
18卷
期数:
2012年01期
页码:
128-133
栏目:
研究论文
出版日期:
2012-02-25

文章信息/Info

Title:
Cultural Characteristics of a Strain of Talaromyces sp. and Its Application in Bioleaching of Sewage Sludge
作者:
周婷袁世斌邹晓莉
(1中国科学院成都生物研究所 成都 610041)
(2中国科学院研究生院 北京 100049)
(3四川大学华西公共卫生学院 成都 610041)
Author(s):
ZHOU Ting YUAN Shibin ZOU Xiaoli
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Graduate University of Chinese Academy of Sciences, Beijing 100049, China)
(3West China School of Public Health, Sichuan University, Chengdu 610041, China)
关键词:
踝节霉菌嗜酸氧化亚铁硫杆菌培养特性HPLC污泥生物淋滤
Keywords:
Talaromyces sp. Acidithiobacillus ferrooxidans cultural characteristics HPLC sludge bioleaching
分类号:
X172
DOI:
10.3724/SP.J.1145.2012.00128
文献标志码:
A
摘要:
于实验室嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)淋滤处理城市污泥酸性废液中分离出一株踝节霉菌ZJ-1,并对其进行了摇瓶液体培养特性研究. 单因素试验结果表明:该菌株的最适碳源和氮源分别为葡萄糖和酵母膏,最适温度为28 ℃,最适转速为140 r/min,最适初始pH值为5.0. 正交试验优化得到碳、氮源的最优水平分别为葡萄糖70 g/L,酵母膏15 g/L. 采用HPLC对该菌株PDA培养液中的有机酸进行了分析,发现其中含有丙酮酸. 初始PDA培养液中丙酮酸含量为0.039 g/L,接菌培养d 5.5时其含量达0.106 g/L. 为探讨踝节霉菌ZJ-1对嗜酸氧化亚铁硫杆菌淋滤污泥过程的影响,在生物淋滤起始阶段接种10% A. ferrooxidans 和4%踝节霉菌ZJ-1,以仅接种10% A. ferrooxidans 作为对照. 结果表明A. ferrooxidans 和踝节霉菌ZJ-1的复合菌组与对照组相比,淋滤耗时由7 d缩短至4 d,淋滤处理时间缩短了43%. 图10 表1 参20
Abstract:
A strain, named as Talaromyces sp. ZJ-1, was isolated from the acid wastewater of sewage sludge with Acidithiobacillus ferrooxidans bioleaching. The liquid fermentation conditions of the strain were studied in rotary flask. The results of the single factor analysis showed that the optimal carbon and nitrogen sources were glucose and yeast extract, respectively. The optimal fermentation temperature, rotation rate and initial pH were 28 ℃, 140 r/min and 5.0, respectively. Orthogonal test indicated that the optimal concentrations of carbon and nitrogen sources were 70 g/L glucose and 15 g/L yeast extract, respectively. HPLC analysis was used to determine organic acids in PDA broth of the strain, and pyruvate was detected with the initial concentrations 0.039 g/L and it reached 0.106 g/L after 5.5 days of culturing. To examine the effect of Talaromyces sp. ZJ-1 on sewage sludge bioleaching, the bioleaching tests of sewage sludge were carried out by inoculating 10% A. ferrooxidans and 4% Talaromyces sp. ZJ-1, and only 10% A. ferrooxidans as control. The results revealed that the required period of bioleaching was shortened from 7 d to 4 d, with 43% less than that of the control. Fig 10, Tab 1, Ref 20

参考文献/References:

1 Chi ZM (池振明). Modern Microbial Ecology. Beijing, China: Science Press (北京: 科学出版社), 2005. 118~119
2 Gao J (高健), Jiang Y (蒋莹), Wu XL (吴学玲), Qiu GZ (邱冠周). Characteristics and molecular identification of a strain of heterotrophic microorganism isolated from acid mine drainage. Microbiology (微生物学通报), 2007, 34 (5): 921~925
3 Han J (韩剑), Chen B (陈波), Dai X (戴欣), Hong W (洪伟), Ji XL (季秀玲), Wei YL (魏云林), Lin LB (林连兵). Diversity of thermoacidophilic Sulfolobus in hot springs in Tengchong of Yunnan, China. Chin J Appl Environ Biol (应用与环境生物学报), 2010, 16 (5): 692~696
4 Xia LX, Yin C, Dai SL, Qiu GZ, Chen XH, Liu JS. Bioleaching of chalcopyrite concentrate using Leptospirillum ferriphilum, Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in a continuous bubble column reactor. J Ind Microbiol Biotechnol, 2010, 37: 289~295
5 Pathak A, Dastidar MG, Sreekrishnan TR. Bioleaching of heavy metals from sewage sludge: A review. J Environ Manage, 2009, 90: 2343~2353
6 Yuan SB (袁世斌), Zhou T (周婷). Influence of Acidithiobacillus thiooxidans HT-8 on solubilization of radionuclide strontium-90 in contaminated soil. Hydrometallurgy China (湿法冶金), 2010, 29 (3): 203~205
7 Hu Y (胡瑜), Bi YL (毕银丽), Zhao B (赵斌). Isolation and identification of a sulfur oxidizing bacterium and its desulphurization of coal wastes. Chin J Appl Environ Biol (应用与环境生物学报), 2007, 13 (1): 116~120
8 Aston JE, Apel WA, Lee BD, Peyton BM. Toxicity of select organic acids to the slightly thermophilic acidophile Acidithiobacillus Caldus. Environ Toxiocol & Chem, 2009, 28 (2): 279~286
9 Zhang ZL (张再利), Shen L (沈镭), Jia XS (贾晓珊). Effects of organic carbon resource on the growth of Thiobacillus ferrooxidans. Acta Sci Nat Univ Sunyatseni (中山大学学报自然科学版), 2006, 45 (6): 121~124
10 Fang D, Zhou LX. Enhanced Cr bioleaching efficiency from tannery sludge with co-inoculation of Acidithiobacillus thiooxidans TS6 and Brettanomyces B65 in an air-lift reactor. Chemosphere, 2007, 69: 303~310
11 Wang SM, Zheng GY, Zhou LX. Heterotrophic microorganism Rhodotorula mucilaginosa R30 improves tannery sludge bioleaching through elevating dissolved CO2 and extracellular polymeric substances levels in bioleach solution as well as scavenging toxic DOM to Acidithiobacillus species. Water Res, 2010, 44: 5423~5431
12 Gu XY, Wong JWC. Degradation of inhibitory substances by heterotrophic microorganisms during bioleaching of heavy metals from anaerobically digested sewage sludge. Chemosphere, 2007, 69: 311~318
13 Romero MC, Hammer E, Hanschke R, Arambarri1 AM, Schauer F. Biotransformation of biphenyl by the filamentous fungus Talaromyces helicus. World J Microbiol & Biotech, 2005, 21: 101~106
14 Romero MC, Reinoso EH, Urrutia MI, Kiernan AM. Biosorption of heavy metals by Talaromyces helicus: A trained fungus for copper and biphenyl detoxification. Electronic J Biotech, 2006, 9 (3): 221~226
15 Fan XR (范秀容), Li GW (李广武), Shen P (沈萍). Microbiology Experiment. Beijing, China: Higher Education Press (北京: 高等教育出版社), 1989. 261~262
16 Huang LH, Schmitt JA. Ohio ascomycete notesⅡ Talaromyces from soils of Southern Ohio. Ohio J Sci, 1975, 75: 75~81
17 Qin ZX (秦忠雪), Li YQ (黎源倩), Yü Q (余倩), Zhao J (赵晋). Determination of metablic organic acids of Bifidobacterium by HPLC. Modern Preventive Med (现代预防医学), 2010, 37 (2): 320~323
18 Zou XL (邹晓莉), Zeng HY (曾红燕), Yan CL (严次玲), Zhu WC (朱万春), Li JY (李继遥). Determination of organic acids in dental plaque with high performance liquid chromatography. West China J Stomatol (华西口腔医学杂志), 2008, 26 (5): 470~472
19 Wang YH (王元好), Dong YS (董悦生), Xiu ZL (修志龙). Rapid determination of organic acids in 1,3-propanediol fermentation broth with gradient elution-high performance liquid chromatography. Food & Ferment Ind (食品与发酵工业), 2010, 36 (1): 126~130
20 Yuan WJ (袁文杰), Kong L (孔亮), Zi LH (孜力汗), Bai FW (白凤武). Simultaneous determination of ethanol, sugar and organic acids in Kluveromyces marxious broth by high performance liquid chromatography-ultraviolet/refractive index detector. Chin J Anal Chem (分析化学研究报告), 2009, 37 (6): 850~854

备注/Memo

备注/Memo:
国家高技术研究发展计划(“863”计划)项目(No. 2006AA06Z351)和中国科学院成都生物研究所领域前沿项目(No. 09S5011100)资助
更新日期/Last Update: 2012-02-29