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[1]文韬,尚慧毅,高志强,等.盐胁迫下Dslhcb3和cao基因的表达及类囊体磷酸化状态转化[J].应用与环境生物学报,2011,17(06):851-854.[doi:10.3724/SP.J.1145.2011.00851]
 WEN Tao,SHANG Huiyi,GAO Zhiqiang,et al.Expression of lhcb3 and cao Gene and Transformation of Phosphorylation State of Thylakoid in Dunaliella salina Under Salt-Stress Condition[J].Chinese Journal of Applied & Environmental Biology,2011,17(06):851-854.[doi:10.3724/SP.J.1145.2011.00851]
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盐胁迫下Dslhcb3和cao基因的表达及类囊体磷酸化状态转化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年06期
页码:
851-854
栏目:
研究论文
出版日期:
2011-12-25

文章信息/Info

Title:
Expression of lhcb3 and cao Gene and Transformation of Phosphorylation State of Thylakoid in Dunaliella salina Under Salt-Stress Condition
作者:
文韬尚慧毅高志强陈卫民李璟李世林乔代蓉曹毅
(四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610064)
Author(s):
WEN TaoSHANG HuiyiGAO ZhiqiangCHEN WeiminLI JingLI ShilinQIAO DairongCAO Yi
(Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, China)
关键词:
盐藻实时PCRlhcb3基因cao基因磷酸酯酶类囊体膜
Keywords:
Dunaliella salinareal-time PCRlhcb3 genecao genephosphatase thylakoid membrane
分类号:
Q949.210.6 : Q78
DOI:
10.3724/SP.J.1145.2011.00851
文献标志码:
A
摘要:
LHCⅡ在在类囊体膜中除了进行光能的吸收和传递之外,在维持类囊体膜的结构,调节激发光能在两个光系统之间的分配,光保护以及对各种环境的适应等过程中都起着重要的作用. 为揭示盐藻LHCB在盐胁迫下的作用机制,以盐生杜氏藻(Dunaliella salina)为材料,应用实时PCR的方法和蛋白电泳技术,研究lhcb3和叶绿素a加氧酶cao基因在低盐胁迫下的表达变化,以及盐藻类囊体膜的磷酸化水平和磷酯酶的变化. 结果表明,在低盐环境下,lhcb3和cao基因的表达活性都呈降低的总趋势,这可能是盐藻适应不同浓度盐环境胁迫的不同反应. 类囊体膜蛋白磷酸化水平先是降低,之后有所升高. 同时,发现磷酸酯酶有着相同的变化趋势,并在12 h时最高. 由此认为低盐条件下,类囊体膜蛋白在前期磷酸化水平的降低与磷酸酯酶活性降低有关,但在12 h后磷酸化水平有所升高,可能是细胞内平衡调节的结果,即磷酸激酶活性增强的结果. 图5 参30
Abstract:
LHCⅡ plays important roles in many processes, such as absorption and transmission of light, maintenance of thylakoid membrance structure, regulation of excitation energy distribution between two optical systems, light protection and adaptation of various environmental protection. In this study, the real-time PCR and protein gel electrophoresis technique were used to study the expression of lhcb3 and chlorophyll a oxygenase gene cao of Dunaliella salina under low-salt stress condition, and the changes of thylakoid membrane phosphorylation and phospholipase. The results showed that under low-salt stress condition, the expression activity of lhcb3 and gene cao decreased, which might result from the adaption responses of D. salina to different concentrations of salt. Phosphorylation of thylakoid membrane protein decreased initially, and increased afterwards. In the mean time, the change of phosphatase had same trend and the peak appeared after 12 h treatment. This study suggested that, under low-salt condition, a lower level of thylakoid membrane protein phosphorylation resulted from decrease of phosphatase activity at the beginning of the treatment. However, after 12 h treatment, in return because of the intracellular homeostasis, the activity of kinase increased, which resulted in the enhancement of phosphorylation eventually. Fig 5, Ref 30

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备注/Memo

备注/Memo:
国家基础性工作专项(No. SB2007FY400-4)、国家自然科学基金项目(No. 30871321)和四川省青年基金资助
更新日期/Last Update: 2011-12-31