|本期目录/Table of Contents|

[1]许玮,冯倩,曹厚利,等.没食子酸丙酯对K562细胞的增殖抑制和诱导凋亡作用[J].应用与环境生物学报,2011,17(04):517-520.[doi:10.3724/SP.J.1145.2011.00517]
 XU Wei,FENG Qian,CAO Houli,et al.Effect of Propyl Gallate on Proliferation Inhibition and Apoptosis Induction in K562 Cells[J].Chinese Journal of Applied & Environmental Biology,2011,17(04):517-520.[doi:10.3724/SP.J.1145.2011.00517]
点击复制

没食子酸丙酯对K562细胞的增殖抑制和诱导凋亡作用()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年04期
页码:
517-520
栏目:
研究论文
出版日期:
2011-08-25

文章信息/Info

Title:
Effect of Propyl Gallate on Proliferation Inhibition and Apoptosis Induction in K562 Cells
作者:
许玮冯倩曹厚利杜林方
(1四川大学生物资源与生态环境教育部重点实验室 成都 610064)
(2四川大学纳米生物医学技术与膜生物学研究所 成都 610041)
Author(s):
XU Wei FENG Qian CAO Houli DU Linfang
(1Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
(2Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu 610041, China)
关键词:
没食子酸丙酯(PG)抗肿瘤乳酸脱氢酶(LDH)K562细胞增殖抑制细胞凋亡赤芍中药
Keywords:
propyl gallate (PG) anti-tumor LDH K562 cell proliferation inhibition apoptosis Paeonia veitchii Chinese medicine
分类号:
R965
DOI:
10.3724/SP.J.1145.2011.00517
文献标志码:
A
摘要:
观察没食子酸丙酯(Propyl gallate,PG)对人慢性髓系白血病K562细胞株的增殖抑制及诱导凋亡作用. 采用倒置显微镜观察细胞形态及数目的变化,乳酸脱氢酶(Lactate dehydrogenase,LDH)释放检测细胞活性,流式细胞术PI染色法及DNA倍体分析检测细胞凋亡作用. 结果表明,没食子酸丙酯能有效地抑制K562细胞增殖,药物处理24 h时细胞形态发生变化,细胞数目减少;细胞培养液中LDH活性分析显示,20~300 ?g/mL没食子酸丙酯处理24 h对细胞毒性作用不明显;但处理时间达48 h时,没食子酸丙酯对细胞毒性明显增加;亚二倍体峰(凋亡峰)的出现及DNA片段化分析表明,200 ?g/mL PG处理细胞24 h时,能引起K562细胞凋亡. 结果表明,PG具有明显的体外抗肿瘤活性,其抗癌活性与其抑制细胞增殖、诱导细胞凋亡有关. 图5 参18
Abstract:
The study was aimed to investigate the effect of propyl gallate (PG) on proliferation inhibition and apoptosis induction in human chronic myeloid leukemia cell line (K562 cells) in vitro. After exposed to PG, morphological changes was detected by inverted microscope and cell number was counted with a hemocytometer. The lactate dehydrogenase (LDH) activity in cell culture was detected by using ultraviolet spectrophotometer with 340 nm. PG-induced apoptosis of K562 cells was analyzed by DNA fragmentation analysis and flow cytometry labeled by propidium iodide (PI). PG showed significant growth inhibition of K562 cells in a dose-dependent manner. Typical morphological change was displayed in PG-treated K562 cells for 24 h. There was no obvious change in LDH activity in cell culture when the cells were treated with PG in the range of 20~300 ?g/mL. However, the LDH activity increased significantly when the cells were incubated with PG from 24 h to 48 h. DNA fragmentation and sub-G1 apoptotic peak analysis indicated that PG induced apoptosis in K562 cells. Fig 5, Ref 18

参考文献/References:

1 Wang JY (王教玉), Zhang QH (张起辉), Deng XM (邓旭明), Wang DC (王大成), Han WY (韩文瑜). 没食子的药理研究进展. Lishizhen Med & Mata Med Res (时珍国医国药), 2007, 18 (10): 2570~2572
2 Karthikeyan K, Sarala BR, Gauthaman K. Protective effect of propyl gallate against myocardial oxidative stress-induced injury in rat. Pharm Pharmacol, 2005, 57 (1): 67~73
3 Reddan JR, Giblin FJ, Sevilla M, Padgaonkar V, Dziedzic DC, Leverenz VR, Misra IC, Chang JS, Peng JT. Propyl gallate is a superoxide dismutase mimic and protects cultured lens epithelial cells from H2O2 insult. Exp Eye Res, 2003, 76 (1): 49~59
4 Zeng R (曾嵘), Li YJ (李元建). Protective effect of propyl gallate on ischemia/reprefusion injmy of rats’ myocardium mtochondrial respiratory enzymes. Practical Prev Med (实用预防医学), 2005, 12 (3): 520~522
5 Zheng JM (郑建明), Chen XC (陈晓春), Zhang J (张静), Lin M (林敏), Huang TW (黄天文), Pan XD (潘晓东). Protective effect of the proply gallate against cerebral ischemia-reperfusion injury via antioxidation in rats. Chin Pharmacol Bul (中国药理学通报), 2008, 24 (7): 942~946
6 Wu TW, Fung KP, Zeng LH, Wu J, Nakamura H. Propyl gallate as a hepatoprotector in vitro and in vivo. Biochem Pharmacol, 1994, 48 (2): 419~422
7 Chen CH, Liu TZ, Chen CH, Wong CH, Chen CH, Lu FJ, Chen SC. The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in IMR-90 cells. Mol Nutr Food Res, 2007, 51 (8): 962~968
8 Boyd I, Beveridge EG. Relationship between the antibacterial activity towards Escherichia coli NCTC 5933 and the physico-chemical properties of some esters of 3,4,5-trihydroxybenzoic acid (gallic acid). Microbios, 1979, 24: 173~184
9 Han YH, Park WH. Propyl gallate inhibits the growth of Hela cells via regulating intracellular GSH level. Food Chem Toxicol, 2009, 47 (10): 2531~2538
10 Sun ZX (孙震晓), Zhang YH (张英慧), Cheng S (程霜), Ma QW (马清温), Guo SL (郭善利), Zhang JB (张金保). Anti-tumor effect of ethanol extracts from Thymus quinquecostatus Celak on human leukemia cell line. J Chin Integr Med (中西医结合学报), 2005, 3 (5): 382~385
11 Boyer RF. Modern experimental biochemistry. 3rd ed. New Jersey, USA: Prentice Hall, 2000. 55~59
12 Kang Z (康庄), Feng Q (冯倩), Cao HL (曹厚利), Wang ZB (王作白), Du LF (杜林方). Antiproliferative effect and induction of apoptosis of trypsin inhibitor isolated from Spinacia oleracea L. in K-562 Cells. Nat Prod Res Dev (天然产物研究与开发), 2009, 21: 684~686
13 Li Z, Liu Y, Zhao X, Pan X, Yin R, Huang C, Chen L, Wei Y. Honokiol, a natural therapeutic candidate, induces apoptosis and inhibits angiogenesis of ovarian tumor cells. Eur J Obstet Gynecol Reprod Biol, 2008, 140 (1): 95~102
14 Mao YQ, Jiang Y, Lei S. Comparison of PI, Annexin V/PI and APO2.7 methods for measuring cellular apoptosis by flow cytometry. West Chin Med J, 2005, 20 (3): 505~506
15 Redlak MJ, Dennis MS, Miller TA. Apoptosis is a major mechanism of deoxycholate-induced gastric mucosal cell death. Am J Physiol Gastrointest Liver Physiol, 2003, 285 (5): G870~879
16 Qian AB (钱安斌). Endothelium-dependent and -independent vascular relaxation induced by propyl gallate in rat aortic rings: [Master diploma thesis]. Hangzhou, China: Zhejiang University (杭州: 浙江大学), 2009
17 Du ZX (都镇先), Liu BQ (刘宝琴), Zhang HY (张海燕), Deng WW (邓娓娓), Wang HQ (王华芹). Proteasome inhibitor-mediated apoptosis mechanism in undifferentiated thyroid cancer cells. Chin J Cancer Prev Treat (中华肿瘤防治杂志), 2010, 17 (2): 85~88
18 Yang YY (杨玉英), Li SZ (李士泽), Yuan XJ (袁学军), Yang HM (杨焕民), Miao SJ (苗树君), Li ZY (李状元). Effects of different environmental temperatures on some biochemical indices in blood of holstein cow. Chin J Appl Environ Biol (应用与环境生物学报), 2002, 8 (2): 171~173

相似文献/References:

[1]丁栋,王博禅,陈桂琛,等.一株抗肿瘤放线菌的鉴定及生物活性[J].应用与环境生物学报,2012,18(06):983.[doi:10.3724/SP.J.1145.2012.00983]
 DING Dong,WANG Bochan,CHEN Guichen,et al.Identification and Bioactivity of an Antitumor Actinomycete[J].Chinese Journal of Applied & Environmental Biology,2012,18(04):983.[doi:10.3724/SP.J.1145.2012.00983]
[2]陈静,刘冉,刘跃文,等.内酯型双乙酰槐糖脂对肿瘤细胞K562的抑制作用[J].应用与环境生物学报,2015,21(02):377.[doi:10.3724/SP.J.1145.2014.08005]
 CHEN Jing,LIU Ran,LIU Yuewen,et al.Inhibition of K562 cancer cells by lactonic diacetyl-sophorolipid[J].Chinese Journal of Applied & Environmental Biology,2015,21(04):377.[doi:10.3724/SP.J.1145.2014.08005]

更新日期/Last Update: 2011-08-16