|本期目录/Table of Contents|

[1]段静波,李少贺,阮琨,等.盐生杜氏藻烯醇酶基因启动子的克隆分析及胁迫转录应答[J].应用与环境生物学报,2011,17(02):202-206.[doi:10.3724/SP.J.1145.2011.00202]
 DUAN Jingbo,LI Shaohe,RUAN Kun,et al.Cloning of Promoter of the Enolase Gene from Duanliella salina and Its Transcriptional Response to Stress[J].Chinese Journal of Applied & Environmental Biology,2011,17(02):202-206.[doi:10.3724/SP.J.1145.2011.00202]
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盐生杜氏藻烯醇酶基因启动子的克隆分析及胁迫转录应答()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年02期
页码:
202-206
栏目:
研究论文
出版日期:
2011-04-25

文章信息/Info

Title:
Cloning of Promoter of the Enolase Gene from Duanliella salina and Its Transcriptional Response to Stress
作者:
段静波 李少贺 阮琨 白林含
(四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都 610064)
Author(s):
DUAN Jingbo LI Shaohe RUAN Kun BAI Linhan
(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
关键词:
盐生杜氏藻烯醇酶基因组步行启动子实时荧光定量PCR胁迫应答
Keywords:
Dunaliella salina enolase genome walking promoter real-time fluorescence quantitative PCR response to stress
分类号:
Q785+Q949.210.5
DOI:
10.3724/SP.J.1145.2011.00202
文献标志码:
A
摘要:
为了解盐生杜氏藻(Dunaliella salina)烯醇酶(Enolase)在渗透耐受中的具体功能,利用基因组步行方法和巢式PCR,从D. salina中克隆了烯醇酶基因DsENO 5’上游约2 000 bp的调控序列,并对其进行序列分析. 分析表明,它包含多个与转录调控有关的保守序列(如CAAT-box,TATA-box),富含光﹑干旱及其它胁迫应答元件. 利用实时荧光定量PCR的方法,研究了高渗﹑高温以及低温外界胁迫条件下DsENO的转录情况,发现其受高渗强烈抑制,高温显著诱导而低温微弱诱导. 图4 表2 参19
Abstract:
With the genome walking method and nested PCR, enolase gene DsENO 5’ upstream regulatory sequence (about 2 000 bp) was cloned from Dunaliella salina, and its sequence analysis was made to investigate the specific function of D. salina during osmotic stress tolerance. The result shows that it includes several conserved sequences related to transcriptional regulation, such as CAAT-box, TATA-box, and it is rich in light, drought and other stress responsive elements. With the real-time fluorescence quantitative PCR method, the DsENO transcription under hyperosmotic, high-temperature and low-temperature stress were studied to find that it was strongly inhibited by hyperosmotic stress and significantly induced by high-temperature, but slightly induced by low-temperature. Fig 4, Tab 2, Ref 19

参考文献/References:

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14 Chitlaru E, Pick U. Selection and Characterization of Dunaliella salina Mutants Defective in Haloadaptation. Plant Physiol, 1989, 91 (2): 788~794
15 Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA. An improved PCR method for walking in uncloned genomic DNA. Nucleic Acids Res, 1995, 23 (6): 1087~1088
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备注/Memo

备注/Memo:
国家自然科学基金项目(Nos. 30500006,30970043)资助
更新日期/Last Update: 2011-04-25