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[1]周宇科,牛向丽,刘永胜.水稻OsGPCR基因的克隆及遗传转化分析[J].应用与环境生物学报,2011,17(01):7-11.[doi:10.3724/SP.J.1145.2011.00007]
 ZHOU Yuke,NIU Xiangli & LIU Yongsheng.Cloning and Genetic Transformation of Rice OsGPCR Gene[J].Chinese Journal of Applied & Environmental Biology,2011,17(01):7-11.[doi:10.3724/SP.J.1145.2011.00007]
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水稻OsGPCR基因的克隆及遗传转化分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年01期
页码:
7-11
栏目:
研究论文
出版日期:
2011-02-25

文章信息/Info

Title:
Cloning and Genetic Transformation of Rice OsGPCR Gene
作者:
周宇科牛向丽刘永胜
(1四川大学生命科学学院生物资源与生态环境教育部重点实验室 成都 610064)
(2重庆大学农学及生命科学研究院 重庆 400030)
Author(s):
ZHOU Yuke1 NIU Xiangli2 & LIU Yongsheng
(1Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
(2Institute of Agricultural and Life Sciences, Chongqing University, Chongqing 400030, China)
关键词:
水稻G蛋白偶联受体7次跨膜结构域农杆菌介导转化赤霉素转基因水稻
Keywords:
OsGPCR seven transmembrane domain Agrobacterium-mediated transformation gibberellin transgenic rice
分类号:
Q943.2 : S511.03
DOI:
10.3724/SP.J.1145.2011.00007
文献标志码:
A
摘要:
G蛋白偶联受体(G protein-coupled receptors,GPCR)基因家族是目前已知最大的膜受体超家族,参与生物的生长发育调控和激素应答. 通过RT-PCR分离了水稻中一个具有7次跨膜(Seven transmembrane,7TM)结构域的OsGPCR候选基因;分析该基因启动子序列,发现它含有赤霉素GA应答相关元件. 为分析其功能,分别构建OsGPCR过表达和RNA干涉载体,并通过农杆菌介导法转化获得转基因水稻植株. 实验结果表明,较之野生型,过表达转基因植株对赤霉素反应更敏感,RNA干涉植株则反之. 图7 表2 参19
Abstract:
G protein-coupled receptors consist of the largest plasma membrane-spanning superfamily known at present, of which the members participate in development regulation and hormone response of organisms. In the present study, a seven-transmembrane (7TM) domain containing candidate GPCR gene was isolated from Oryza sative L. Nipponbare by RT-PCR. The analysis of the upstream sequence suggested that OsGPCR might be involved in gibberellin (GA) signaling pathway. To analyze the function of OsGPCR, over-expression and RNA interference vectors were constructed and transgenic rice lines were obtained by Agrobacterium-mediated transformation. The results indicated that the over-expressing OsGPCR transgenic plants were more sensitive to gibberellin compared to wild type, whereas RNA interference lines showed less sensitivity. Fig 7, Tab 2, Ref 19

参考文献/References:

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备注/Memo

备注/Memo:
国家杰出青年科学基金项目(No. 30825030)和国家转基因生物新品种培育科技重大专项课题(Nos. 2009ZX08001-011B,2009ZX08009-072B)资助
更新日期/Last Update: 2011-02-28