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[1]GABRIEL Ugwemorubong Ujagwung,OBOMANU Faithwin Gamaliel,EDORI Onisogen Simon.Biochemical Changes in Hybrid Catfish (Heterobranchus bidorsalis, ♂ x Clarias gariepinus, ♀) Treated with Nuracron[J].应用与环境生物学报,2010,16(03):353-357.[doi:10.3724/SP.J.1145.2010.00353]
 GABRIEL Ugwemorubong Ujagwung,OBOMANU Faithwin Gamaliel,EDORI Onisogen Simon.Biochemical Changes in Hybrid Catfish (Heterobranchus bidorsalis, ♂ x Clarias gariepinus, ♀) Treated with Nuracron[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):353-357.[doi:10.3724/SP.J.1145.2010.00353]
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Biochemical Changes in Hybrid Catfish  (Heterobranchus bidorsalis, ♂ x Clarias gariepinus, ♀) Treated with Nuracron()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
353-357
栏目:
研究论文
出版日期:
2010-06-25

文章信息/Info

Title:
Biochemical Changes in Hybrid Catfish  (Heterobranchus bidorsalis, ♂ x Clarias gariepinus, ♀) Treated with Nuracron
作者:
GABRIEL Ugwemorubong Ujagwung OBOMANU Faithwin Gamaliel EDORI Onisogen Simon
(1Department of Fisheries and Aquatic Environment, Rivers State University of Science & Technology, P.M.B 5080, Port Harcourt, 5000001, Nigeria)
(2Department of Chemistry, Rivers State University of Science & Technology, P.M.B 5080, Port Harcourt 5000001, Nigeria)
(3Department of Chemistry, Rivers State University of Education, P.M.B. 5047, Rumuolumeni, Port Harcourt. 5000001, Nigeria)
Author(s):
GABRIEL Ugwemorubong Ujagwung OBOMANU Faithwin Gamaliel EDORI Onisogen Simon
(1Department of Fisheries and Aquatic Environment, Rivers State University of Science & Technology, P.M.B 5080, Port Harcourt, 5000001, Nigeria)
(2Department of Chemistry, Rivers State University of Science & Technology, P.M.B 5080, Port Harcourt 5000001, Nigeria)
(3Department of Chemistry, Rivers State University of Education, P.M.B. 5047, Rumuolumeni, Port Harcourt. 5000001, Nigeria)
关键词:
aspartate transaminase alanine transaminase alkaline phosphatase hybrid catfish nuracron
Keywords:
aspartate transaminase alanine transaminase alkaline phosphatase hybrid catfish nuracron
分类号:
X174
DOI:
10.3724/SP.J.1145.2010.00353
文献标志码:
A
摘要:
The effects of sublethal levels of nuracron, dimethylphosphate-3-hydroxy-N-methyl-cis-crotonamide (0, 0.625×10-6, 1.25×10-6, 2.5×10-6, 5.0×10-6 and 10.0×10-6) on biochemical changes (aspartate transaminase, AST, E. C. 2.6.1.1; alanine transaminase, ALT; 2.6.2.2; alkaline phosphatase, ALP, EC 3.1.3.1) in the organs (kidney, liver, gill) and muscle tissues of hybrid catfish Heterobranchus bidorsalis, ♂ × Clarias gariepinus, ♀ [mean weight, (277.76±53.11) g SD; mean total length, (35.69±2.80) cm, SD] were studied after a 23 d exposure in a renewal bioassay under laboratory conditions. Generally, nuracron inhibited AST, ALT and ALP activities in all the exposure concentrations, with ALT activity being mostly affected. The most elicited enzyme was ALP in the kidney [(4 052.67±1610.45) IU/L], followed by AST in the muscle [(908.44±218.34) IU/L] and ALT in the liver [(160.29±73.68) IU/L]. The ALP activity was 30.24 and 6.53 times greater than that of ALT and AST. The highest inhibition for AST (43.65%), ALT (47.88%) and ALP (57.98%) occurred at 10×10-6, 0.625×10-6 and 2.5×10-6, respectively. The activities of the enzymes in all the organs did not show any direct relationship with the exposure concentrations of the toxicant. ALT activity in the muscle generally suffered the most compared with the other enzymes. The activities of enzymes in the liver were generally inhibited, with elevation at 5.0×10-6 for all the enzymes. The lower concentrations of nuracron (0.625×10-6~2.5×10-6) caused elevation in the activities of ALP, but the reverse was the case with the higher concentrations; these of AST and ALT were haphazard. The relative activities of the various enzymes in the organs showed that the order for AST was liver>muscle>kidney>gill; that for ALT: liver>kidney, muscle>gill and that for ALP: kidney>gill>liver and muscle. However, for AST and ALT the activities in the organs of the exposed fish peaked at 5.0×10-6 nuracron. The alteration in the activities of the enzymes indicated that the extract interfered with the Kreb’s cycle intermediaries and the transamination process, and therefore could elicit negative consequences on the physiology of the fish, a non-target species in the aquatic environment. Fig 3, Tab 5, Ref 27
Abstract:
The effects of sublethal levels of nuracron, dimethylphosphate-3-hydroxy-N-methyl-cis-crotonamide (0, 0.625×10-6, 1.25×10-6, 2.5×10-6, 5.0×10-6 and 10.0×10-6) on biochemical changes (aspartate transaminase, AST, E. C. 2.6.1.1; alanine transaminase, ALT; 2.6.2.2; alkaline phosphatase, ALP, EC 3.1.3.1) in the organs (kidney, liver, gill) and muscle tissues of hybrid catfish Heterobranchus bidorsalis, ♂ × Clarias gariepinus, ♀ [mean weight, (277.76±53.11) g SD; mean total length, (35.69±2.80) cm, SD] were studied after a 23 d exposure in a renewal bioassay under laboratory conditions. Generally, nuracron inhibited AST, ALT and ALP activities in all the exposure concentrations, with ALT activity being mostly affected. The most elicited enzyme was ALP in the kidney [(4 052.67±1610.45) IU/L], followed by AST in the muscle [(908.44±218.34) IU/L] and ALT in the liver [(160.29±73.68) IU/L]. The ALP activity was 30.24 and 6.53 times greater than that of ALT and AST. The highest inhibition for AST (43.65%), ALT (47.88%) and ALP (57.98%) occurred at 10×10-6, 0.625×10-6 and 2.5×10-6, respectively. The activities of the enzymes in all the organs did not show any direct relationship with the exposure concentrations of the toxicant. ALT activity in the muscle generally suffered the most compared with the other enzymes. The activities of enzymes in the liver were generally inhibited, with elevation at 5.0×10-6 for all the enzymes. The lower concentrations of nuracron (0.625×10-6~2.5×10-6) caused elevation in the activities of ALP, but the reverse was the case with the higher concentrations; these of AST and ALT were haphazard. The relative activities of the various enzymes in the organs showed that the order for AST was liver>muscle>kidney>gill; that for ALT: liver>kidney, muscle>gill and that for ALP: kidney>gill>liver and muscle. However, for AST and ALT the activities in the organs of the exposed fish peaked at 5.0×10-6 nuracron. The alteration in the activities of the enzymes indicated that the extract interfered with the Kreb’s cycle intermediaries and the transamination process, and therefore could elicit negative consequences on the physiology of the fish, a non-target species in the aquatic environment. Fig 3, Tab 5, Ref 27

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更新日期/Last Update: 2010-06-23