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[1]韩庆莉,赵志瑞,李元,等.类球红细菌对敌敌畏暴露中斑马鱼的保护作用[J].应用与环境生物学报,2010,16(03):358-362.[doi:10.3724/SP.J.1145.2010.00358]
 HAN Qingli,ZHAO Zhirui,LI Yuan,et al.Protection of Zebrafish Against Dichlorvos by Rhodobacter sphaeroides[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):358-362.[doi:10.3724/SP.J.1145.2010.00358]
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类球红细菌对敌敌畏暴露中斑马鱼的保护作用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
358-362
栏目:
研究论文
出版日期:
2010-06-25

文章信息/Info

Title:
Protection of Zebrafish Against Dichlorvos by Rhodobacter sphaeroides
作者:
韩庆莉赵志瑞李元王谦王栋齐鸿雁白志辉
(1云南农业大学资源与环境学院 昆明 650201)
(2中国科学院生态环境研究中心 北京 100085)
(3西南林学院基础部 昆明 650224)
(4中科活力(北京)生物技术有限公司 北京 100012)
Author(s):
HAN Qingli ZHAO Zhirui LI Yuan WANG Qian WANG Dong QI Hongyan BAI Zhihui
(1College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China)
(2Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China)
(3Fundamental Department, Southwest Forestry College, Kunming 650224, China)
(4Zhongke-Huoli (Beijing) Biotechnology Corporation, Beijing 100012, China)
关键词:
敌敌畏斑马鱼急性毒性类球红细菌生物降解
Keywords:
dichlorvos DDVP zebrafish acute toxicity Rhodobacter sphaeroides biodegradation
分类号:
X172 : X174
DOI:
10.3724/SP.J.1145.2010.00358
文献标志码:
A
摘要:
敌敌畏是广泛使用的有机磷农药之一,具有较高的水溶性,对水生动物的危害较大. 为探讨敌敌畏降解菌对水体中中毒鱼类的降毒效果,测定了敌敌畏对斑马鱼(Brachydanio rerio)的急性毒性,研究了一株敌敌畏高效降解菌——类球红细菌(Rhodobacter sphaeroides)的解毒作用. 结果显示:敌敌畏对斑马鱼24、48、72、96 h的LC50分别为42.9、37.5、30.7、25.6 mg L-1,安全浓度为2.6 mg L-1,属低毒农药;在不同敌敌畏暴露浓度的养鱼水中添加类球红细菌(5×107 CFU mL-1)可以显著降低斑马鱼的死亡率,28.3 mg L-1和33.6 mg L-1敌敌畏暴露浓度下,斑马鱼96 h死亡率分别由对照组(不添加类球红细菌)的63.3%和96.7%降低到0;利用液相色谱检测了敌敌畏在添加和不添加类球红细菌的养鱼水中的降解规律,结果表明类球红细菌对斑马鱼的保护作用主要是快速降解水中的敌敌畏,并且没有产生对鱼有毒的降解产物. 本研究获得了敌敌畏对斑马鱼的基础毒理学数据,同时为微生物降解水体中的农药残留提供了科学依据. 图3 表1 参19
Abstract:
Dichlorvos (DDVP) is one of widely used organophosphate insecticides, with high water solubility and high risk to aquatic animals. In order to explore the detoxification of poisoned fish by DDVP using degrading strains, the acute toxicity of DDVP to zebrafish (Brachydanio rerio) was determined, and the detoxification by using a strain of Rhodobacter sphaeroides with high degradation capability to DDVP was investigated. The result showed that the half lethal concentration (LC50) of DDVP to zebrafish within 24, 48, 72 and 96 h were 42.9, 37.5, 30.7 and 2.6 mg L-1, respectively. The safe concentration was 2.56 mg L-1 which showed that DDVP belonged to low poisonous pesticide to zebrafish. Adding R. sphaeroides (5×107 CFU mL-1) to 28.3 mg L-1 and 33.6 mg L-1 of DDVP-exposed fish water reduced zebrafish mortality significantly from 63.3% and 96.7% both to 0 within 96 h. The degradation of DDVP was detected by HPLC in fish water with and without R. sphaeroides respectively. The result showed that the detoxification by R. sphaeroides was mainly due to DDVP degradation without poisonous degradation products to fish. Fig 3, Tab 1, Ref 19

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备注/Memo

备注/Memo:
国家科技支撑计划项目(Nos. 2009BAC57B02,2007BAC27B01)和环境水质学国家重点实验室专项基金(No. 08K09ESPCR)资助 Supported by the State Science & Technology Program of China (Nos. 2009BAC57B02, 2007BAC27B01) and the Special Fund from the State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-environmental Sciences, Chinese Academy of Sciences(No. 08K09ESPCR)
更新日期/Last Update: 2010-06-23