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[1]张文静,魏继雨,罗会涛,等.池塘浮游生物优势种组成与DNA指纹的关系[J].应用与环境生物学报,2010,16(05):672-677.[doi:10.3724/SP.J.1145.2010.00672]
 ZHANG Wenjing,WEI Jiyu,LUO Huitao,et al.Relations Between DNA Fingerprinting Data and Dominant Species Composition of Pond Plankton Communities[J].Chinese Journal of Applied & Environmental Biology,2010,16(05):672-677.[doi:10.3724/SP.J.1145.2010.00672]
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池塘浮游生物优势种组成与DNA指纹的关系()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年05期
页码:
672-677
栏目:
研究论文
出版日期:
2010-10-25

文章信息/Info

Title:
Relations Between DNA Fingerprinting Data and Dominant Species Composition of Pond Plankton Communities
文章编号:
200911032
作者:
张文静魏继雨罗会涛田野
(1中国地质大学环境学院,生物地质与环境地质教育部重点实验室,生态环境研究所 武汉 430074)
(2厦门大学海洋与环境学院 厦门 361005)
Author(s):
ZHANG WenjingWEI JiyuLUO HuitaoTIAN Ye
(1Institute of Eco-environment, Key Laboratory of Biogeology and Environmental Geology of Ministry of Education, School of Environmental Studies, China University of Geosciences, Wuhan 430074, China)
(2College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China)
关键词:
浮游生物优势种遗传关系RAPDDNA指纹多态性池塘
Keywords:
plankton dominant species genetic relations RAPD DNA fingerprinting polymorphism pond
分类号:
Q178.1
DOI:
10.3724/SP.J.1145.2010.00672
文献标志码:
A
摘要:
研究中国地质大学(武汉)校区3个池塘7个样点浮游生物群落的优势种组成和DNA指纹多态性. 利用15种随机引物分别扩增,其中7种引物获得多态性片段. 电泳共得到47条带,谱带平均多态率为96.4%. 根据Nei’s遗传距离矩阵构建3个池塘7个样点的遗传关系聚类树. 通过优势种群组成聚类分析比较探讨浮游生物优势物种组成和DNA指纹遗传关系聚类树结构的关系. 结果表明,同一池塘样点的优势种组成和DNA指纹遗传多样性相似性较高;西区池塘样点的优势种组成和遗传关系最近,其次是东区池塘的3个样点,差别最大的是北区池塘的两个样点. 其原因可能是生境的隔离和差异. 提示尽管池塘浮游生物优势种组成与DNA指纹结构表示的遗传关系会存在较小差异,但是分析结果基本一致. 因此,利用DNA指纹技术研究池塘浮游生物生态学是可行的. 图5 表3 参12
Abstract:
Seven plankton community samples were collected from three ponds in the campus of China University of Geosciences in Wuhan, Hubei, China. All the samples were analyzed with RAPD fingerprinting polymorphism and the dominant species of plankton communities were identified. 15 random primers were amplified and 7 primers obtained RAPD polymorphic fragments. 47 bands were obtained after the electrophoresis and average band polymorphism rate was 96.4%. The genetic relation trees of the seven points in the three ponds were constructed according to the matrix of Nei’s genetic distances. Relations between cluster trees of DNA fingerprinting topological structure and dominant species of plankton communities in the three ponds were discussed. The results showed that each pool had the similar plankton dominant species community and genetic relationship. The dominant plankton species community and genetic relations from the sampling sites in the west pond were most similar, and followed by the three sites in the east pond were followed. Those from the two sites in the north pond were most different. The reason might be that their living environments were different. Genetic similarity results based on DNA fingerprinting topological structure were in accord with the clustering results of dominant plankton species community except a small difference. Therefore, DNA fingerprinting technique is feasible to study the plankton ecology of ponds. Fig 5, Tab 3, Ref 12

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金项目(No. 30800097)、中国科学院知识创新工程重要方向项目(No. KZCX2-YW-QN401)、生物地质与环境地质教育部重点实验室开放课题(No. BGEG0810)、中国地质大学(武汉)优秀青年教师资助计划项目(No. CUGQNL0801)和中央高校基本科研业务费专项资金(No. CUG090103)资助 Supported by the National Natural Science Foundation of China (No. 30800097), the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KZCX2-YW-QN401), the Open Fund of Key Laboratory of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences (No. BGEG0810), the Research Foundation for Outstanding Young Teachers, China University of Geosciences,Wuhan, China (No. CUGQNL0801) and the Special Fund for Basic Scientific Research of Central Colleges of China (No. CUG090103)
更新日期/Last Update: 2010-10-25