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[1]王霞,刘铭钧,高俊娥,等.太阳鱼脑乙酰胆碱酯酶的分离纯化[J].应用与环境生物学报,2011,17(01):135-137.[doi:10.3724/SP.J.1145.2011.00135]
 WANG Xia,LIU Mingjun,GAO June & LI Dun.Purification of Acetylcholinerase from Lepomis gibbosus Brain[J].Chinese Journal of Applied & Environmental Biology,2011,17(01):135-137.[doi:10.3724/SP.J.1145.2011.00135]
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太阳鱼脑乙酰胆碱酯酶的分离纯化()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
17卷
期数:
2011年01期
页码:
135-137
栏目:
研究简报
出版日期:
2011-02-25

文章信息/Info

Title:
Purification of Acetylcholinerase from Lepomis gibbosus Brain
作者:
王霞刘铭钧高俊娥李盾
(华南农业大学资源环境学院 广州 510642)
Author(s):
WANG Xia LIU Mingjun GAO Jun’e & LI Dun
(College of Resources & Environment, South China Agricultural University, Guangzhou 510642, China)
关键词:
乙酰胆碱酯酶太阳鱼纯化亲和柱层析
Keywords:
acetylcholinesterase (AChE) Lepomis gibbosus (sunfish) purification affinity chromatography
分类号:
X174 : Q566
DOI:
10.3724/SP.J.1145.2011.00135
文献标志码:
A
摘要:
采用硫酸铵沉淀–普鲁卡因胺亲和柱层析方法,对太阳鱼(Lepomis gibbosus)脑部乙酰胆碱酯酶分离纯化,纯化倍数为234倍,回收率为25.67%,经SDS-PAGE电泳检测,呈现单一的条带,相对分子质量约为68 000,表明其纯度达到电泳纯. 图4 表1 参16
Abstract:
The acetylcholinesterase (AChE, EC 3.1.1.7) from sunfish (Lepomis gibbosus) brain was purified by ammonium sulfate and procainamide affinity chromatography. The purification fold reached 234 with activity recovery rate of 25.67%. By means of the SDS-PAGE analysis, the purified AChE preparation showed one band, and its relative molecular mass was about 68 000, which indicate the high degree of purification. Fig 4, Tab 1, Ref 16

参考文献/References:

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备注/Memo:
广东省科技计划项目(No. 2007B080401018)资助
更新日期/Last Update: 2011-02-28