|本期目录/Table of Contents|

[1]梅嬛,洪青,李顺鹏.一株孔雀石绿降解菌的分离鉴定及降解特性[J].应用与环境生物学报,2010,16(03):390-393.[doi:10.3724/SP.J.1145.2010.00390]
 MEI Huan,HONG Qing,LI Shunpeng.Isolation, Identification and Characterization of a Malachite Green-degrading Bacterium[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):390-393.[doi:10.3724/SP.J.1145.2010.00390]
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一株孔雀石绿降解菌的分离鉴定及降解特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
390-393
栏目:
研究论文
出版日期:
2010-06-25

文章信息/Info

Title:
Isolation, Identification and Characterization of a Malachite Green-degrading Bacterium
作者:
梅嬛洪青李顺鹏
(南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室 南京 210095)
Author(s):
MEI Huan HONG Qing LI Shunpeng
(Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Sciences,
Nanjing Agricultural University, Nanjing 210095, China)
关键词:
孔雀石绿生物降解降解途径Raoultella sp.废水处理
Keywords:
malachite green biodegradation degrading pathway Raoultella sp. wastewater treatment
分类号:
X172
DOI:
10.3724/SP.J.1145.2010.00390
文献标志码:
A
摘要:
从某化工厂的废水处理池污泥中分离到一株孔雀石绿降解菌,命名为K4-W. 根据生理生化特征和16S rRNA基因序列相似性分析,将其初步鉴定为Raoultella sp. 该菌在48 h内对20 mg/L的孔雀石绿降解率为82.5%,降解孔雀石绿的最适温度为30 ℃,外加碳源或Pb2+对其降解有明显的促进作用,增大接种量也有利于提高降解效率,较高浓度的孔雀石绿则对降解有抑制作用. 该菌在降解过程中没有生成无色孔雀石绿,这不同于已报道的孔雀石绿降解途径,但该菌是否具有新的降解途径还需进一步研究. 图3 参24
Abstract:
A bacterial strain capable of degrading malachite green was isolated from the sludge sample collected from wastewater treating system of a chemical plant, and it was designated as strain K4-W. According to the similarity analysis of its 16S rRNA gene sequence (GenBank Accession No. EU876828) and physiological-biochemical characteristics, it was preliminarily indentified as Raoultella sp. Strain K4-W could degrade 82.5% of 20 mg/L malachite green within 48 h. The optimal temperature for the degradation was 30 ℃. The addition of Pb2+ or other carbon sources could promote the degradation significantly. The increasing in inoculum also showed promoting effect on the degradation. The high concentration of malachite green would inhibit the degradation. No leucomalachite green appeared during the degradation process, which was different from the reported malachite green degrading pathway. Therefore, a further study was needed to verify whether strain K4-W harbored a new degradation pathway. Fig 3, Ref 24

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备注/Memo

备注/Memo:
国家“863计划”项目(No. 2007AA061101)和科技部自然科技资源平台项目(No. 2005DKA21201-2)资助 Supported by the National High Technology Research and Development Program of China (No. 2007AA061101) and the National Technology Platform Program of the Ministry of Science & Technology of China (2005DKA-21201-2)
更新日期/Last Update: 2010-06-23