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[1]冯晓虎,朱卫华,吴弘,等.若尔盖高原湿地纤维素降解菌组成及产酶特性[J].应用与环境生物学报,2010,16(03):399-402.[doi:10.3724/SP.J.1145.2010.00399]
 FENG Xiaohu,ZHU Weihua,WU Hong,et al.Composition and Enzyme-producing Property of Cellulolytic Microbes in High Altitude Wetland of Zoige, China[J].Chinese Journal of Applied & Environmental Biology,2010,16(03):399-402.[doi:10.3724/SP.J.1145.2010.00399]
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若尔盖高原湿地纤维素降解菌组成及产酶特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年03期
页码:
399-402
栏目:
研究论文
出版日期:
2010-06-25

文章信息/Info

Title:
Composition and Enzyme-producing Property of Cellulolytic Microbes in High Altitude Wetland of Zoige, China
作者:
冯晓虎朱卫华吴弘谢洁张书巨艳乔代蓉曹毅
(1成都生物制品研究所 成都 610023)
(2四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065)
Author(s):
FENG Xiaohu ZHU Weihua WU Hong XIE Jie ZHANG Shu JU Yan QIAO Dairong CAO Yi
(1Chengdu Institute of Biological Products, Chengdu 610023, China)
(2Microbiology and Metabolic Engineering Key laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China)
关键词:
纤维素降解菌野油菜黄单胞菌若尔盖湿地青藏高原
Keywords:
cellulose degrading microbe Xanthomonas campestris Zoige wetland Qinghai-Tibet Plateau
分类号:
Q939.9 : S154.3
DOI:
10.3724/SP.J.1145.2010.00399
文献标志码:
A
摘要:
以青藏高原湿地若尔盖地区不同生境土壤为材料,通过富集,计算降解圈个数,分析该地区纤维素降解菌的含量. 分离得到15株具有纤维素降解能力的菌株,从中复筛得到一株降解能力较强的菌株3C-6.12,经16S rRNA和Biolog鉴定为野油菜黄单胞菌(Xanthomonas campestris). 该菌株在以质量比例3 : 2的麸皮与玉米芯为发酵培养基时,酶活最高为66.44 U/mL,最适pH值为6~8,最佳生长温度为30 ℃. 该菌株的成功选育为后续高效、充分利用纤维素类物质生产生物能源提供了很好的菌种来源. 图7 表1 参17
Abstract:
Zoige with abundant waterweeds is famous for wetland of the Qinghai-Tibet Plateau, and various kinds of cellulose-degrading microorganisms occur in the soil of this region. The composition of cellulolytic microbes were analyzed through enrichment and the number of transparent circles (TC) was calculated. 15 strains were obtained from the samples collected in the different habitats in Zoige and a strain named 3C-6.12 with strong cellulose degrading capability was screened from them. Biolog and 16S rRNA molecular identification confirmed that the strain was Xanthomonas campestris. The highest activity (66.44 U/mL) of cellulase was present when the bran/corncob ratio was 3 : 2 used in fermentation medium. The optimum growth pH and temperature of strain 3C-6.12 were 6~8 and 30 ℃, respectively. Cellulose is the richest organic material on the earth. Therefore, with this strain cellulose can fully and effectively used to produce bioenergy. Fig 7, Tab 1, Ref 17

参考文献/References:

1 Béguin P, Aubert JP. The biological degradation of cellulose. FEMS Microbiol Rev, 1994, 13 (1): 25~58
2 Sanderson K. US biofuels: A field in ferment. Nature, 2006, 444: 673~676
3 Goldemberg J. Ethanol for a sustainable energy future. Science, 2007, 315: 808~810
4 Tomme P, Warren RA, Gilkes NR. Cellulose hydrolysis by bacteria and fungi. Adv Microb Physiol, 1995, 37: 1~81
5 Bhat MK. Cellulases and related enzymes in biotechnology. Biotechnol Adv, 2000, 18 (5): 355~383
6 Sohail M, Siddiqi R, Ahmad A, Khan SA. Cellulase production from Aspergillus niger MS82: Effect of temperature and pH. N Biotechnol, 2009, 25 (6): 437~441
7 Yang X (杨霞), Zai XL (翟兴礼), Yu GY (余国莹). Current stuation of Roige plateau wetlands biodiversity and their conservation countermeasure. J Changchun Univ (长春大学学报), 2002, 12 (3): 16~20
8 Liu XD, Yin ZY. Sensitivity of East Asian monsoon climate to the uplift of the Tibetan Plateau. Palaeogeogr Palaeoclimatol Palaeoecol, 2002, 183: 223~245
9 Kongruang S. Bacterial cellulose production by Acetobacter xylinum strains from agricultural waste products. Appl Biochem Biotechnol, 2008, 148: 245~256
10 Long W (龙雯), Chen CS (陈存社). Application of 16S rRNA sequence in bacteria indication. J Beijing Technol & Business Univ (北京工商大学学报), 2006, 24 (5): 10~12
11 Cheng C (程池), Yang M (杨梅), Li JX (李金霞), Yao S (姚粟), Hu HR (胡海蓉). Biolog microbial identification system-study on the operating regulation of bacteria identification biolog. Food & Ferment Inds (食品与发酵工业), 2006, 32 (5): 50~54
12 Huang XP (黄筱萍), Liu L (刘兰). Studies on selection of Xanthomonas campestris mutant X.c-18 and the fermentation conditions for xanthan production. Jiangxi Sci (江西科学), 1997, 15 (1): 13~20
13 Chen HG (陈红歌), Chen J (陈健), Wang X (王旭), Hu Y (胡渝), Ma XD (马向东). Study on properties of α-amylase from Xanthomonas campestris pv. Campestris 8004. J Northwest A & F Univ Nat Sci Ed (西北农林科技大学学报自然科学版), 2005, 33 (2): 143~146
14 Xie DP (谢丹平), Yin H (尹华), Peng H (彭辉), Ye JS (叶锦韶), Zhang N (张娜). Degradation of crude oil by mixed culture. Chin J Appl Environ Biol (应用与环境生物学报), 2004, 10 (2): 210~214
15 Wang Q (王乾), Bao WK (包维楷), Yan ZL (晏兆莉), Timo K, Alfred C, Angela M. Basic types and characters of the western zoige meadows and their changes in recent decades. Chin J Appl Environ Biol (应用与环境生物学报), 2002, 8 (2): 133~141
16 Wang DX (王德宣), Song CC (宋长春), Wang YS (王跃思), Wang YY (王毅勇), Yan BX (阎百兴), Zhao ZC (赵志春). Carbon dioxide flux from peat mire in Ruoergai plateau. Ecol & Environ (生态环境), 2005, 14 (6): 880~883
17 Wang DX (王德宣), lv XG (吕宪国),Ding WX (丁维新), Cai ZC (蔡祖聪),Gao JF (高景福), Yang FM (杨福明). Methane emission from marshes in Zoige Plateau. Adv Earth Sci (地球科学进展), 2002, 17 (6): 877~879

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备注/Memo

备注/Memo:
国家基础性工作专项(No. SB2007FY400-4)和国家重点基础研究发展计划项目(973项目,No. 2009CB125910)资助 Supported by the National Special Basic Research Project of China (No. SB2007FY400-4) and the National Basic Research and Development Program of China (973 Program, No. 2009CB125910)
更新日期/Last Update: 2010-06-23