|本期目录/Table of Contents|

[1]李铭,杨亚力,杨顺楷.响应面法优化固定粘红酵母细胞条件及生物转化制L-苯丙氨酸[J].应用与环境生物学报,2010,16(02):279-284.[doi:10.3724/SP.J.1145.2010.00279]
 LI Ming,YANG Yali,YANG Shunkai.Optimal Conditions for Bio-production of L-phenylalanine by Immobilized Cells of Rhodotolula glutinis[J].Chinese Journal of Applied & Environmental Biology,2010,16(02):279-284.[doi:10.3724/SP.J.1145.2010.00279]
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响应面法优化固定粘红酵母细胞条件及生物转化制L-苯丙氨酸()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年02期
页码:
279-284
栏目:
技术与方法
出版日期:
2010-04-25

文章信息/Info

Title:
Optimal Conditions for Bio-production of L-phenylalanine by Immobilized Cells of Rhodotolula glutinis
文章编号:
200906025
作者:
李铭杨亚力杨顺楷
(1中国科学院成都生物研究所 成都 610041)
(2四川省产品质量监督检验检测院 成都 610031)
Author(s):
LI MingYANG YaliYANG Shunkai
(1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
(2Sichuan Institute of Product Quality Supervision & Inspection, Chengdu 610031, China)
关键词:
响应面法优化条件固定粘红酵母细胞生物转化L-苯丙氨酸
Keywords:
response surface methodology optimization immobilized cells Rhodotorula glutinis CIBAS 06112-8-3 bio-production L-phenylalanine
分类号:
TQ922
DOI:
10.3724/SP.J.1145.2010.00279
文献标志码:
A
摘要:
采用响应面分析法对影响粘红酵母细胞固定化的4个主要因素——单体浓度、交联剂浓度、包埋量、包埋时间进行了优化. 首先用Plackett-Burman法设计实验对以上4个影响因素进行了评价,并筛选出显著影响因素——单体浓度和包埋量;第2步用最陡爬坡实验逼近以上两因素最优水平;最后由中心复合实验及响应面分析确定主要影响因素的最佳条件. 固定化粘红酵母细胞的最优包埋条件为:单体浓度16.7%,包埋量43%,交联剂浓度2.0%,包埋时间45 min. 在此条件下得到的固定化细胞与游离细胞相比,酶活保留率可达63%,较优化前提高了31%. 实验室制备试验中L-苯丙氨酸(L-Phe)积累浓度可达53.3 g/L,与该菌株的中试游离整细胞一次批式转化产L-Phe浓度30 g/L比较,产率提高了1.8倍. 图3 表9 参15
Abstract:
Placked-Burman (PB) design, path of steepest ascent (PSA), and central composite design (CCD) were used to select and optimize four factors, i.e., monomer concentration, crosslinking agent concentration, embedded amount in the media and embedding time for the conditions of bio-production of L-phenylalanine (L-Phe) by the immobilized cells of Rhodotorula glutinis CIBAS 06112-8-3 used as biocatalysts. Firstly, two key factors, the monomer concentration and embedded amount in the media, were found significant via PB method. Secondly, according to the following statistic analysis of the yield and activity of the immobilized cells of the strain, PSA was adopted to approach the optimal region. Finally, CCD design and response surface analysis were to determine the optimal levels of the main factors. Therefore, there were the optimal conditions for the immobilized cells, including monomer concentration of 16.7%, embedded amount in the media of 43%, crosslinking agent concentration of 2.0% and embedded time of 45 min, and the recovery of the immobilized cells’ activity reached at 63% and increased by 31%, compared with control treatment. In addition, the yield of L-Phe increased 1.8 times with a general whole cells, i.e., a total amount of L-Phe accumulated in several batches of reactive media, reached 53.3 g/L in laboratory scale as compared with 30 g/L in pilot scale. Fig 3, Tab 9, Ref 15

参考文献/References:

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备注/Memo

备注/Memo:
常州常茂生物化学工程股份有限公司资助 Supported by the Changmao Biochemical Engineering Co. Ltd., Changzhou, China
更新日期/Last Update: 2010-04-20