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[1]李晓晨,李华鹏,邓喜,等.BnGID1基因过量表达对甘蓝型油菜矮化突变体NDF-1株高的恢复[J].应用与环境生物学报,2010,16(02):192-196.[doi:10.3724/SP.J.1145.2010.00192]
 LI Xiaochen,LI Huapeng,DENG Xi,et al.Plant Height Recovery Caused by BnGID1 Over-expression in a Dwarf Mutant NDF-1 of Brassica napus L.[J].Chinese Journal of Applied & Environmental Biology,2010,16(02):192-196.[doi:10.3724/SP.J.1145.2010.00192]
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BnGID1基因过量表达对甘蓝型油菜矮化突变体NDF-1株高的恢复()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年02期
页码:
192-196
栏目:
研究论文
出版日期:
2010-04-25

文章信息/Info

Title:
Plant Height Recovery Caused by BnGID1 Over-expression in a Dwarf Mutant NDF-1 of Brassica napus L.
文章编号:
200904030
作者:
李晓晨李华鹏邓喜马欣荣王茂林
(1四川大学生命科学学院 成都 610064)
(2中国科学院成都生物研究所 成都 610041)
Author(s):
LI XiaochenLI HuapengDENG XiMA XinrongWANG Maolin
(1College of Life Sciences, Sichuan University, Chengdu 610064, China)
(2Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)
关键词:
甘蓝型油菜BnGID1基因矮化突变体过量表达载体遗传转化株高恢复
Keywords:
Brassica napus L. BnGID1 gene dwarf mutant over-expression vector genetic transformation plant height recovery
分类号:
S635.03 : Q78
DOI:
10.3724/SP.J.1145.2010.00192
文献标志码:
A
摘要:
在正常株高油菜中克隆了与拟南芥矮化突变相关基因AtGID1同源的BnGID1基因全长cDNA目的片段,将其正向插入表达载体pFGC5941上的CaMV35S启动子下游,构成该基因过量表达载体pFGC5941-BnGID1. 经根癌农杆菌介导,导入甘蓝型油菜矮化突变体NDF-1基因组,通过PCR及半定量RT-PCR检测,确定得到抗性转基因植株23株. 移至大田生长,观察结果显示,转基因植株明显高于对照矮化突变体NDF-1,平均高出49.05 cm. 结果表明,BnGID1基因过量表达能够使甘蓝型油菜矮化突变体NDF-1株高得到较大程度的恢复. 图7 表2 参16
Abstract:
A full-length cDNA of BnGID1 gene with 1 041 bp was cloned from Brassica napus L. BnGID1 is a homolog of Arabidopsis AtGID1 which was confirmed to encode a receptor protein of gibberellin. For addressing the function of BnGID1 gene, an over-expression binary vector pFGC5941-BnGID1 was constructed on basis of the binary plasmid pFGC5941 in which BnGID1 was driven by CaMV35S promoter. The dwarf ‘NDF-1’ plants were transformed via Agrobacterium tumefaciens containing pFGC5941-BnGID1. Twenty-three transgenic plants were then identified by PCR and semi-quantification RT-PCR. Grown in field, the heights of the transgenic plants and dwarf mutants were measured, respectively. The results showed that the transgenic plants were significantly higher than the plants of dwarf mutant NDF-1 with an average height of 49.05 cm, indicating that the BnGID1 gene over-expression could partially recover plant height of dwarf mutant NDF-1. Fig 7, Tab 2, Ref 16

参考文献/References:

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相似文献/References:

[1]解艳芳,牛显飞,蒋学飞,等.RNA-seq比较甘蓝型油菜矮化突变体与其高秆亲本的可变剪切[J].应用与环境生物学报,2018,24(01):81.[doi:10.19675/j.cnki.1006-687x.2017.04016]
 XIE Yanfang,NIU Xianfei,JIANG Xuefei,et al.Comparison of alternative splicing of $Brassica napus$ dwarf mutant and its tall-stalked parents by using RNA-seq[J].Chinese Journal of Applied & Environmental Biology,2018,24(02):81.[doi:10.19675/j.cnki.1006-687x.2017.04016]

备注/Memo

备注/Memo:
四川省农作物育种科技攻关项目(No. 2006YZGG-5-1)资助 Supported by the Crop Breeding Project of Sichuan, China (No. 2006YZGG-5-1)
更新日期/Last Update: 2010-04-20