|本期目录/Table of Contents|

[1]赵祖国,孔庆鑫,王景峰,等.一种羟胺氧化酶基因序列的PCR扩增及其生物信息学分析[J].应用与环境生物学报,2009,15(01):100-105.[doi:10.3724/SP.J.1145.2009.00100]
 ZHAO Zuguo,KONG Qingxin,WANG Jingfeng,et al.PCR Amplification and Bioinformatics of a Novel Gene Coding Hydroxylamine Oxidoreductase[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):100-105.[doi:10.3724/SP.J.1145.2009.00100]
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一种羟胺氧化酶基因序列的PCR扩增及其生物信息学分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年01期
页码:
100-105
栏目:
研究论文
出版日期:
2009-02-25

文章信息/Info

Title:
PCR Amplification and Bioinformatics of a Novel Gene Coding Hydroxylamine Oxidoreductase
作者:
赵祖国孔庆鑫王景峰金敏王新为李君文
中国人民解放军军事医学科学院卫生学与环境医学研究所 天津 300050
Author(s):
ZHAO Zuguo KONG Qingxin WANG Jingfeng JIN Min WANG Xinwei LI Junwen
Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050, China
关键词:
不动杆菌生物信息学生物脱氮氨氮PCR羟胺氧化酶基因序列开放读码框
Keywords:
acinetobacter bioinformatics biological denitrification ammonia nitrogen PCR hydroxylamine oxidoreductasegene sequence open read frame
分类号:
X172 : Q78
DOI:
10.3724/SP.J.1145.2009.00100
文献标志码:
A
摘要:
采用PCR法获得不动杆菌Acinetobactor sp. YY-5的一种羟胺氧化酶(Hydroxylamine oxidoreductase, HAO)编码基
因序列并进行生物信息学分析. 根据自养菌的HAO基因序列保守区设计4对引物,通过RT-PCR获得预期大小的部分序
列,再采用Genome walking PCR法向所得的部分序列两侧延伸;对所得序列在NCBI中进行blast分析,并用FGeneSB进
行开放读码框(Open read frame,ORF)预测. 结果表明,RT-PCR获得了一段462 bp的序列,通过Genome walking PCR向
两侧延伸后,获得了一段3 152 bp长的序列;ORF预测结果显示所得序列可能有4个ORF,RT-PCR获得的462 bp序列所在
ORF长555 bp,对应氨基酸序列相对分子质量(Mr)大小为20.2×103;在Genebank中进行的Blast比对分析显示,除保守区
的引物序列外,未发现有与此ORF存在明显相似性的HAO基因,表明这可能是一种新型羟胺氧化酶基因. 图5 表3 参14
Abstract:
A probable novel gene coding hydroxylamine oxidoreductase (HAO) was acquired by PCR and gene
sequencing in Acinetobactor sp. YY-5. Four pairs of PCR primers were designed according to the conservative regions of
reported HAO gene to amplify and sequence the partial sequence of the HAO gene; several primers for genome walking
PCR were designed according to the partial sequence to acquire the flank region of the partial HAO sequence; and the
acquired sequence was subjected to Blast analysis in NCBI and open read frame (ORF) prediction by the professional
ORF -analyzing software, FGeneSB. A 462 bp DNA sequence was obtained by one of the 4 pairs of primers, and further
2 690 bp DNA sequence of the flank region of 462 bp sequence was acquired through two rounds of genome walking
PCR. Four ORFs were found within the 3 152 bp DNA sequence, one of which was of 555 bp. The 555-bp ORF contained the original 462 bp sequence and corresponded to an amino acid sequence of Mr 20.2×103, and no obvious similar HAO gene was found in GenBank. Thus, a novel HAO gene was possibly acquired by PCR. Fig 5, Tab 3, Ref 14

参考文献/References:

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Biochemistry, 1981, 20 (24): 7026~7032
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备注/Memo

备注/Memo:
国家自然科学基金项目(No . 3 0 6 0 0 4 8 7 ),国家“ 8 6 3”项目(No. 2006AA06Z334)和天津市应用基础研究计划项目(No. 07JCYBJC07000)资助
更新日期/Last Update: 2009-03-05