|本期目录/Table of Contents|

[1]金福厚,庞岩,李士泽,等.BALB/C鼠睾丸组织中冷诱导RNA结合蛋白的cDNA克隆与序列分析[英文][J].应用与环境生物学报,2009,15(01):87-90.[doi:10.3724/SP.J.1145.2009.00087]
 JIN Fuhou,PANG Yan,LI Shize,et al.Cloning and Sequence Analysis of Cold Inducible RNA-binding Protein cDNA from Testis Tissue in BALB/C Mice[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):87-90.[doi:10.3724/SP.J.1145.2009.00087]
点击复制

BALB/C鼠睾丸组织中冷诱导RNA结合蛋白的cDNA克隆与序列分析[英文]()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年01期
页码:
87-90
栏目:
研究论文
出版日期:
2009-02-25

文章信息/Info

Title:
Cloning and Sequence Analysis of Cold Inducible RNA-binding Protein cDNA from Testis Tissue in BALB/C Mice
作者:
金福厚庞岩李士泽杨焕民计红赵巧香尹位
1黑龙江八一农垦大学动物科技学院 大庆 163319
2黑龙江强尔生化技术开发有限公司 哈尔滨 150090
Author(s):
JIN Fuhou PANG Yan LI Shize YANG Huanmin JI Hong ZHAO Qiaoxiang YIN Wei
1College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing 163319, Heilongjiang, China
2Heilongjiang Qiang’er Biochemical Development Co., Ltd., Harbin 150090, China
关键词:
冷诱导RNA结合蛋白cDNA克隆冷应激序列分析
Keywords:
CIRP cDNA cloning cold stress sequence analysis
分类号:
Q78 : Q494
DOI:
10.3724/SP.J.1145.2009.00087
文献标志码:
A
摘要:
冷诱导RNA结合蛋白(Cold inducible RNA-binding protein, CIRP)在多种冷应激细胞(包括重组中国仓鼠卵巢细
胞)中被发现. 迄今为止,冷应激对活体生物基因表达的影响还未见报道. 和细胞相比,生物体具有更加复杂的冷应
激调节机制. 本研究以冷处理的BALB/C鼠为实验动物,从其睾丸组织中克隆出了CIRP的cDNA. 结果表明,CIRP在生
物体中能够被低温诱导,可能防止生物体遭受冷损伤. 根据克隆的cDNA所推测的氨基酸序列与GenBank上公布的小
鼠、大鼠、人类、牛蛙、美西螈、非洲爪蟾胚胎细胞和卵母细胞的CIRP氨基酸序列同源性分别为100%、99.4%、95.5%、
67.4%、58.4%、76.9% 和79.1% . 这表明CIRP在生物进化过程中是高度保守的,可能具有多种生理功能. 因此,这一研究
将为探索人类和动物冷应激分子机制创立系统试验模型和奠定新的实践基础. 图5 参14
Abstract:
The cold-inducible RNA-binding protein (CIRP) was found in various cells including recombinant Chinese hamster
ovary (rCHO) cells under cold stress. However, the effect of cold stress on the gene expression of the intravital animals has not been reported till now. Compared with their cells, there were much more complicated regulatory mechanisms for cold stress response in the organisms. The BALB/C mice with cold treatment were used as experimental animals for this study. The cDNA of CIRP was firstly cloned from the testis tissues of the BALB/C mice treated by cold stress. The results indicated that CIRP in the organisms could be induced at low temperature and might protect the organisms from the cold damage. The amino acid sequences deduced via cDNA clone were 100%, 99.4%, 95.5%, 67.4%, 58.4%,76.9%, and 79.1% identical to those of the CIRP in mice, rats, human, bullfrog and axolotl cells, and Xenopus embryos and oocytes, respectively. These results show that the CIRP is highly conserved in the evolution process and may be involved in various physiological functions. Therefore, this study will establish a systematic model for experiments and provide a new foundation for exploring the molecular mechanisms of human and animals under cold stress. Fig 5, Ref 14

参考文献/References:

1 Hiroyuki N, Katsuhiko I, Yoshiyuki K, Masamichi K, Osamu Y, Jun F. A
glycine-rich RNA-binding protein mediating cold-inducible suppression
of mammalian cell growth. J Cell Biol, 1997,137 (4): 899~908
2 Dörthe MK. On heat and cells and proteins. News Physiol Sci, 2004, 19:
11~15
3 Nuray NU, Ferhan ET. Cold shock proteins. Turk J Med Sci, 2001, 31:
283-290.
4 Ken Matsumoto, Kazuma Aoki, Naoshi Dohmae, Koji Takio, Masafumi
Tsujimoto. CIRP2, a major cytoplasmic RNA-binding protein in Xenopus
oocytes. Nucl Acids Res, 2000, 28 (23): 4689~4697
5 Nishiyama H, Danno S, Kaneko Y, Itoh K, Yokoi H, Fukumoto M, Okuno
H, Millan JL, Matsuda T, Yoshida O, Fujita J. Decreased expression
of cold-inducible RNA-binding protein (CIRP) in male germ cells at
elevated temperature. Am J Pathol, 1998, 152 (1): 289~296
6 Nishiyama H, Xue JH, Sato T, Fukuyama H, Mizuno N, Houtani T,
Sugimoto T, Fujita J. Diurnal change of the cold-inducible RNA-binding
protein (Cirp) expression in mouse brain. Biochem Biophys Res Commun,
1998, 245 (2): 534~538
7 Uochi T, Asashima M. XCIRP (Xenopus homolog of cold-inducible
RNA-binding protein) is expressed transiently in developing pronephros
and neural tissue. Gene, 1998, 211 (2): 245~250
8 Xue JH, Nonoguchi K, Fukumoto M, Sato T, Nishiyama H, Higashitsuji
H, Itoh K, Fujita J. Effects of ischemia and H2O2 on the cold stress protein
CIRP expression in rat neuronal cells. Free Radic Biol Med, 1999, 27
(11/12): 1238~1244
9 Saito T, Sugimoto K, Adachi Y, Wu Q, Mor i KJ. Cloning and
characterization of amphibian cold inducible RNA-binding protein.
Comp Biochem Physiol B Biochem Mol Biol, 2000, 125 (2): 237~245
10 Peng Y, Kok KH, Xu RH, Kwok KH, Tay D, Fung PC, Kung HF, Lin
MC. Maternal cold inducible RNA binding protein is required for
embryonic kidney formation in Xenopus laevis. FEBS Lett, 2000, 482:
37~43
11 Atia A, Hamid AA, Mandai M, Nanbu K, Kariya M, Kusakari T,
Fukuhara K, Fujii S. Expression of cold inducible RNA-binding protein
in the normal endometrium, endometrial hyperplasia, and endometrial
carcinoma. Int J Gynecol Pathol, 2003, 22: 240~247
12 Sakurai T, Itoh K, Higashitsuji H, Nonoguchi K, Liu Y, Watanabe H,
Nakano T, Fukumoto M, Chiba T, Fujita J. Cirp protects against tumor
necrosis factor-α-ind-uced apoptosis activation of extracellular signalregulated
kinase. Biochim Biophys Acta, 2006, 1763: 290~295
13 Nishiyama H, Higashitsuji H, Yokoi H, Itoh K, Danno S, Matsuda T,
Fujita J. Cloning and characterization of human CIRP (cold-inducible
RNA-binding protein) cDNA and chromosomal assignment of the gene.
Cene, 1997, 204: 115~120
14 Dreyfuss G, Matunis MJ, Pinol, RS, Burd CG. HnRNP proteins and the
biogenesis of mRNA. Annu Rev Biochem, 1993, 62: 289~321

相似文献/References:

[1]赵巧香,张秀玲,纪丽丽,等.重组BALB/C鼠冷诱导RNA结合蛋白CIRP多克隆抗体的制备与鉴定[J].应用与环境生物学报,2009,15(05):634.[doi:10.3724/SP.J.1145.2009.00634]
 ZHAO Qiaoxiang,ZHANG Xiuling,JI Lili,et al.Preparation and Identification of Recombinant Protein of CIRP’S Polyclonal Antibody in BALB/C Mice[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):634.[doi:10.3724/SP.J.1145.2009.00634]
[2]李静辉,孟宇,李玉恒,等.冷诱导RNA结合蛋白(CIRP)过表达载体的构建及鉴定[J].应用与环境生物学报,2015,21(02):381.[doi:10.3724/SP.J.1145.2014.08010]
 LI Jinghui,MENG Yu,LI Yuheng,et al.Construction and identification of over-expressing vector of cold inducible RNA-binding protein (CIRP)[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):381.[doi:10.3724/SP.J.1145.2014.08010]
[3]刘炜婳,林玉玲,赖钟雄.福州宦溪野生蕉CBF7的cDNA与启动子克隆及在不同温度下的表达特性[J].应用与环境生物学报,2017,23(2):200.[doi:10.3724/SP.J.1145.2016.03032]
 LIU Weihua,LIN Yuling & LAI Zhongxiong**.Cloning and expression profile of the cDNA and promoter of CBF7 in wild banana (Musa itinerans) from Huanxi of Fuzhou City[J].Chinese Journal of Applied & Environmental Biology,2017,23(01):200.[doi:10.3724/SP.J.1145.2016.03032]

备注/Memo

备注/Memo:
Supported by the National Postdoctoral Science Foundation of China (Grant No. 20060390241) and the Postdoctoral Science Foundation of Heilongjiang, China
更新日期/Last Update: 2009-03-05