|本期目录/Table of Contents|

[1]张海泉,符海霞,马淑琴.粗山羊草间杂交及抗条锈病新基因遗传分析和分子标记[J].应用与环境生物学报,2009,15(01):44-47.[doi:10.3724/SP.J.1145.2009.00044]
 ZHANG Haiquan,FU Haixia,MA Shuqin.Intraspecific Hybridization among Aegilops tauschii Varieties & Genetic Analysis and Molecular Markers of a Novel Stripe Rust Resistance Gene from A. tauschii[J].Chinese Journal of Applied & Environmental Biology,2009,15(01):44-47.[doi:10.3724/SP.J.1145.2009.00044]
点击复制

粗山羊草间杂交及抗条锈病新基因遗传分析和分子标记()
分享到:

《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
15卷
期数:
2009年01期
页码:
44-47
栏目:
研究论文
出版日期:
2009-02-25

文章信息/Info

Title:
Intraspecific Hybridization among Aegilops tauschii Varieties & Genetic Analysis and Molecular Markers of a Novel Stripe Rust Resistance Gene from A. tauschii
作者:
张海泉符海霞马淑琴
1河北经贸大学生物科学与工程学院 石家庄 050061
2天津医科大学 天津 300070
3云南省农业科学院粮食作物研究所 昆明 650205
Author(s):
ZHANG Haiquan FU Haixia MA Shuqin
1College of Biology Engineering, Hebei University of Economics and Business, Shijiazhuang 050061, China
2Tianjin Medical University, Tianjing 300070, China
3Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205,China
关键词:
粗山羊草杂交小麦条锈病基因定位微卫星标记
Keywords:
Aegilops tauschii hybrid wheat stripe rust gene location simple sequence repeats (SSR)
分类号:
S512.103.4
DOI:
10.3724/SP.J.1145.2009.00044
文献标志码:
A
摘要:
粗山羊草是小麦野生近缘属种,是D基因组的供体,蕴含大量的抗病资源,是进行小麦遗传改良的重要资源.
选取条锈病免疫材料Y206和高度感病材料Y121杂交后代进行遗传分析和抗病性鉴定. 从粗山羊草[Aegilops tauschii
(Coss.) Schmal] Y206中鉴定出1个显性抗小麦条锈病基因,暂定名为YrY206. 并利用SSR分子标记对该抗病基因标记定
位,应用分离群体分组法(Bulked segregant analysis, BSA)筛选到Wmc11a、Xgwm71c、Xgwm161和xgwm183标记,与该基因之间的遗传距离分别为4.0 cM、3.3 cM、1.5 cM和9.3 cM. 根据连锁标记所在小麦微卫星图谱的位置,YrY206被定位
在3DS染色体上,可能是一个新的抗小麦条锈病基因. 图2 表2 参22
Abstract:
As the wild relatives of wheat and D genome donor of wheat, Aegilops tauschii with many resistant resources,
and is an important genetic resource for wheat breeding. Genetic analysis and disease resistance identification were made for the wheat stripe rust of filial generation from immune material Y206 and high sensitive Y121. A dominant wheat stripe rust resistance gene was detected from A. tauschii (Coss.) Schmal Y206. The novel gene was temporarily designated as YrY206. Using the microsatellite marker location on the chromosome, by bulk segregation analysis, four microsatellite markers Wmc11a, Xgwm71c, Xgwm161 and Xgwm183 were found and linked to YrY206 with genetic distances of 4.0, 3.3, 1.5 and 9.3 cM, respectively. According to the locations of the linked markers, the resistance gene was located on chromosome 3DS and YrY206 may be a novel stripe rust resistance gene. Fig 2, Tab 2, Ref 22

参考文献/References:

1 McIntosh RA, Lagudah ES. Cytogenetical studies in wheat. XVIII. Gene
Yr24 for resistance to stripe rust. Plant Breeding, 2000, 119 (1): 76~81
2 Singh RP, Nelson JC, Sorrells ME. Mapping Yr28 and other genes for
resistance to stripe rust in wheat. Crop Sci, 2000, 40: 1148~1155
3 Zhang HQ (张海泉), Fu XT (符晓棠), Hao CY (郝晨阳), Zhang BS (张
宝石). Progress of studies on powdery mildew resistance genes in wheat.
J Shenyang Agric Univ (沈阳农业大学学报), 2003, 34 (1): 68~71
4 Zhang HQ (张海泉), Jia JZ (贾继增), Zhang BS (张宝石), Zhou RH (周
荣华). Chromosomal localization of powdery mildew resistance gene
PmAeY1 from Ae. tauschii Y219. Rev China Agric Sci & Technol (中国农
业科技导报), 2006, 8 (4): 19~22
5 Zhang HQ (张海泉). SSR markers linked to a powdery mildew resistance
gene in Aegilops tauschii Y189. J Henan Univ Nat Sci (河南大学学报自
然科学版), 2007, 37 (2): 177~180
6 Miranda LM, Murphy JP, Marshall D, Leath S. Pm34: A new powdery
mildew resistance gene transferred from Aegilops tauschii Coss to
common wheat ( Triticum aestivum L). Theor & Appl Genet,2006, 113
(8): 1497~1504
7 Miranda LM, Murphy JP, Marshall D, Cowger C, Leath S. Chromosomal
图1 SSR标记Wmc11a在F2群体部分单株中的分布
Fig. 1 Distribution of SSR marker Wmc11a in the F2 segregation population
M: Marker; 1: 抗病亲本; 2: 感病亲本; 3: 抗病池; 4: 感病池; 5~33: 抗病单株; 34~42: 感病单株
M: Marker PBR322 DNA/MSP marker; 1: Resistance parent; 2: Susceptible parent; 3: resistant bulk; 4: susceptible bulk; 5~33: Phenotypically resistant
individuals; 34~42: Phenotypically susceptible individuals
图2 抗条锈病基因YrY206的遗传连锁图(3D)
Fig. 2 Linkage map of stripe rust resistance gene YrY206 on chromosome 3D
location of Pm35, a novel Aegilops tauschii derived powdery mildew
resistance gene introgressed into common wheat (Triticum aestivum L. ).
Theor & Appl Genet, 2007, 114 (8): 1451~1456
8 Lutz JE, Limpert PB, Zeller FJ. Identification of powdery mildew
resist ance genes in common wheat (Trit icum ae st ivum L.) I.
Czechoslovakian cultivars. Plant Breeding, 1992, 108: 33~39
9 Aldrich C. CTAB DNA extraction from plant tissues. Plant Molecular
Biology Reporter, 1993, 11: 128~141
10 Röder MS, Korzum V, Wendehake K, Plaschke J, Tixier MH, Leroy
P, Ganal MW. A microsatellite map of wheat. Genetics, 1998b, 149:
2007~2023
11 Michelmore RW, Paran I, Kesseli RV. Identification of markers linked to
disease resistance genes by using segregating analysis: A rapid method
to detect markers in specific genomic regions by using segregating
populations. Proc Nat Acad Sci U S A, 1991, 88: 9828~9832
12 Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln
SE, Newburg L. MAPMAKER: An interactive computer package
for constructing primary genetic map of experimental and natural
populations. Genomics, 1987, 1: 174~181
13 McIntosh RA, Hart GE, Devos KM, Rogers WJ. Morris CF, Appels R,
Anderson OD. Catalogue of gene symbols for wheat: 2007 supplement.
http: //shigen. lab. nig. ac. jp//wheat/komugi/top/top. jsp. 2007
14 Sun XL, Liu D, Zhang HQ, Jia JZ. Identification and mapping of two
new genes conferring resistance to powdery mildew from Aegilops
tauschii (Coss. ) Schmal. Plant Biol, 2006, 48 (10): 1204~1209
15 Kosambi DD. The estimation of map distances from recombination
values. Ann Eugenics, 1994, 12: 172~175
16 Bai D, Knot DR. Suppression of rust resistance in bread wheat (Triticum
aesitivum L.) by 2D genome chromosomes. Genome, 1992, 35: 276~282
17 Gill BS, Raupp WJ. Direct genetic transfer from Aegilops squerrosa L.
to hexaploid wheat. Crop Sci, 1987, 27: 445~450
18 Li GQ, Li ZF, Yang WY, Zhang Y, He ZH, Xu SC, Singh RP, Qu YY,
Xia XC. Molecular mapping of stripe rust resistance gene YrCH42 in
Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and
Yr26. Theor & Appl Genet, 2006, 112 (8): 1434~1440
19 Kuraparthy V, Chhuneja P, Dhaliwal HS, Kaur S,Bowden RL,Gill
BS. Characterization and mapping of cryptic alien introgression from
Aegilops geniculata with new leaf rust and stripe rust resistance genes
Lr57 and Yr40 in wheat. Theor & Appl Genet, 2007, 114 (8): 1379~1389
20 Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES. Powdery mildew
resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe
rust cosegregate at a locus on the short arm of chromosome 7D of wheat.
Theor & Appl Genet, 2005, 111 (4): 731~735
21 Yan GP, Chen XM. Molecular mapping of a recessive gene for resistance
to stripe rust in barley. Theor & Appl Genet, 2006, 113 (3): 529~537
22 He MZ (何名召), Wang LM (王丽敏), Zhang ZY (张增艳), Xu SC
(徐世昌), Wang LL (王丽丽), Xin ZY (辛志勇). Identification and
molecular mapping of a novel stripe rust resistance gene in a triticum
durum-Aegilops tauschii amphiploid CI108. Acta Agron Sin (作物学报).
2007, 33 (7): 1045~1050

相似文献/References:

[1]王杰.日本大鲵的现状及对中国大鲵保护的启示[J].应用与环境生物学报,2015,21(04):683.[doi:10.3724/SP.J.1145.2014.10025]
 WANG Jie.Current status of Japanese giant salamander and the enlightenment on the conservation of Chinese giant salamander[J].Chinese Journal of Applied & Environmental Biology,2015,21(01):683.[doi:10.3724/SP.J.1145.2014.10025]

备注/Memo

备注/Memo:
河北省科技厅河北省科学技术研究与发展指导计划“微卫星标记小麦野
生资源抗病基因及抗病基因的利用”项目(No. 05225510)资助
更新日期/Last Update: 2009-03-05