|本期目录/Table of Contents|

[1]任燕萍,覃建兵.转基因小麦麦谷蛋白亚基突变体类型研究[J].应用与环境生物学报,2010,16(01):54-57.[doi:10.3724/SP.J.1145.2010.00054]
 REN Yanping,ZHU Changqing,QIN Jianbing.Study on Mutation Type of HMW-GS Component in transgenic wheat progenies[J].Chinese Journal of Applied & Environmental Biology,2010,16(01):54-57.[doi:10.3724/SP.J.1145.2010.00054]
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转基因小麦麦谷蛋白亚基突变体类型研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
16卷
期数:
2010年01期
页码:
54-57
栏目:
研究论文
出版日期:
2010-02-25

文章信息/Info

Title:
Study on Mutation Type of HMW-GS Component in transgenic wheat progenies
作者:
任燕萍覃建兵
(新疆师范大学分子生物学与生物信息学研究室 乌鲁木齐 830053)
Author(s):
REN Yanping ZHU Changqing QIN Jianbing
(Research Division of Molecular Biology and Informatics, Xinjiang Normal University, Urumqi 830053, China)
关键词:
突变类型SDS-PAGE转基因小麦麦谷蛋白等位变异
Keywords:
mutation type SDS-PAGE transgenic wheat glutenin allelic variation
分类号:
Q78 : S512.103.3
DOI:
10.3724/SP.J.1145.2010.00054
文献标志码:
A
摘要:
采用十二烷基磺酸钠–聚丙烯酰胺凝胶电泳技术,分析了含外源1Dx5和1Ax1的T7代转基因小麦HMW-GS的突变类型及等位变异. 结果显示,随机挑选的1 000份B72-8-11b T6代籽粒在T7代中共21类突变类型,其外源1Dx5基因表达频率为49.4%,Glu A1、Glu B1和Glu D1共3、13和9个等位变异类型,分别以N、17+18和N为主,占94.7%、61.5%和49.7%;B102-1-2共11类,外源1Ax1基因表达频率占95%,Glu A1、Glu B1和Glu D1共2、7和3个等位变异类型,分别以1、17+18和N为主,占95%、98.7%和98.3%。研究结果进一步说明了转基因株系的纯合性,完全符合孟德尔遗传分离规律,对小麦优质新品种的培育具有理论意义和应用价值. 图2 表4 参9
Abstract:
Mutation types of HMW-GS component in independent transgenic wheat lines were investigated in this paper. The results showed that there were 21 categories in the 1 000 selected randomly of B72-8-11b, the expression of 1Dx5 gene accounted for 49.4%, three types were detected on Glu A1, and N subunit was the major types with frequency of 94.7%, thirteen types were detected on Glu B1, and 17+18 subunit was the major types with frequency of 61.5%, nine types were detected on Glu D1, and N subunit was the major types with frequency of 49.7%; There were 11 categories in the B102-1-2, the expression of 1Ax1 gene accounted for 95%, two types were detected on Glu A1, and N subunit was the major types with frequency of 95%, seven types were detected on Glu B1, and 17+18 subunit was the major types with frequency of 98.7%, three types were detected on Glu D1, and N subunit was the major types with frequency of 98.3%. The results also indicated that the transmission of the transgene and inheritance of its expression followed Mendelian ratios with the method of quantitative genetics, and T7 generation lines almost homozygous. It would be beneficial for deciding the selection strategy in breeding program. Fig 2, Tab 4, Ref 9

参考文献/References:

1 Qin JB (覃建兵), Zhu CQ (祝长青), Zhao HX (赵惠新). Measures of interesting gene 1Dx5 expression in transgenic wheat progenies. J Huazhong Norm Univ (华中师范大学学报), 2008, 42 (2): 267~271
2 Li SH (李三和), Li J (李举), Wang NL (王娜丽), He GY (何光源). Variation of HMW-GS expresssion resulted by the foreign 1Dx5 gene. Lett Biotechnol (生物技术通讯), 2007, 18 (2): 217~219
3 Wang NL (王娜丽), Wang YS (汪越胜), Li J (李举), Li SH (李三和), He GY (何光源). Studies on transgenes 1Dx5 and 1Ax1 transferred into elite wheat cultivars by cross breeding. J Wuhan Bot Res (武汉植物学研究), 2007, 25 (4): 331~334
4 Huang DY, Khan K. Quantitative determination of high molecular weight glutenin subunit of hard red spring wheat by SDS-PAGE: Quantitative effects of total amounts on bread making quality characteristics. Cereal Chem, 1997, 76: 781~788.
5 Zhang XM, Xu HJ, Du LP, Ye XG, Xin ZY, Guo AG, Xue S, Ma YZ. Excision of bar gene from transgenic wheat obtained by biolistic co-transformation. Acta Agron Sin, 2004 , 30 (1): 26~30
6 He G Y, Rooks L, Steele H S, Bekes F, Gras P, Tatham A S, Fido R, Barcelo P, Shewry P R, Lazzeri P A. Transformation of pasta wheat (Triticum turgidum L. var. durum) with high-molecular-weight glutenin subunit genes and modification of dough functionality. Mol Breeding, 1999, 5: 377~386
7 Wang YS (汪越胜), Qin JB (覃建兵), Chang JL (常俊丽), Fang JY (房敬业), He GY (何光源), Peter Shewry. Study on heritance of foreign gene 1Dx5 expressed in transgenic wheat. Mol Plant Breeding (分子植物育种), 2005, 3 (4): 451~455
8 Wang YS (汪越胜), He T (柯涛), Li SH (李三和), Qin JB (覃建兵), Liu Y (刘勇), He GY (何光源). Variation of HMW-GS component in the progeny of transgenic wheat (Triticum aestivum L.). Acta Agron Sin (作物学报), 2005, 31 (4): 529~531
9 Pan ZF (潘志芬), Deng GB (邓光兵), Liu Y (刘毅), Wu F (吴芳), Yu MX (余懋群). Composition of high molecular wheat glutenin subunits and effect on wheat baking quality. Chin J Appl Environ Biol (应用与环境生物学报), 2006, 12 (2): 155~159

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备注/Memo

备注/Memo:
国家自然科学基金项目(No. 30960092),科技支疆项目(No. 200840102),新疆维吾尔自治区高校科研计划重点项目(No. XJEDU2007S27)和新疆师范大学研究生创新基金资助
更新日期/Last Update: 2010-02-09