|本期目录/Table of Contents|

[1]黄河清,张凤章,邱雪慧,等.固氮酶单、双钼铁钼辅基的制备和特性[J].应用与环境生物学报,1997,3(03):258-262.
 Huang Heqing,Zhang Fengzhang,Qiu Xuehui,et al.PREPARATION AND CHARACTERIZATION OF FeMo COFACTORS CONTAINING SINGLE AND DOUBLE Mo FROM NITROGENASE[J].Chinese Journal of Applied & Environmental Biology,1997,3(03):258-262.
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固氮酶单、双钼铁钼辅基的制备和特性()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
3卷
期数:
1997年03期
页码:
258-262
栏目:
论文
出版日期:
1997-08-25

文章信息/Info

Title:
PREPARATION AND CHARACTERIZATION OF FeMo COFACTORS CONTAINING SINGLE AND DOUBLE Mo FROM NITROGENASE
作者:
黄河清1 张凤章1 邱雪慧1 林庆梅2
1. 厦门大学生物学系 厦门 361005;
2. 厦门大学环境科学研究中心 厦门 361005
Author(s):
Huang Heqing Zhang Fengzhang Qiu Xuehui Li Qingmei
1. Department of Biology, Xiamen University, Xiamen 361005;
2. Research Center of Environment Science, Xiamen University, Xiamen 361005
关键词:
固氮酶铁钼辅基单、双钼铁硫簇光谱分析
Keywords:
NitrogenaseFeMo cofactorFe-S clusters containing single and double Mospectral analysis
摘要:
固氮酶单、双钼铁钼辅基的制备与N-甲基甲酰胺碱度有关.质子激发X射线发射光谱和电感耦合等离子体发射光谱分析技术测定单、双钼铁钼辅基的钼铁流元素组成比值均为1:6:6.在紫外可见光谱区内,单、双钼铁钼辅基均无特征吸收峰,不含高柠檬酸盐。
Abstract:
Preparation of FeMo cofactors containing single and double Mo was related with basicity of N-methylformamide. The mininal elemental composition of FeMo cofactor containing single and double Mo was found to be Mo: 6Fe: 6S by using inductively coupled plasma and proton induced X-ray emission analytical technique. F6Mo cofsctors containing single and double Mo showed featureless absorbence peak in the ultraviolet and visible spectral range and did not contain homocitrate.

参考文献/References:

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2. Watt GD, Huang HQ, Reddy KRN. Redox properties of the nitrogenase protein. Molebdennm Enzymes cofactor and Model Systems. 1993, 535:243~256
3. Shah VK,Brill WJ.lsolation of iron-molybdenum cofactor from nitrogenase. Proc. Nall Acda Sci USA. 1977, 74: 3249~3253
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7. 黄河清,Watt GD.固氮酶铁钼辅基理化特性的研究.生物化学杂志.1995. 11(4):465~469
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9. Huang HQ,Watt RK, Frankel RK et al.Role of phosphate in Fe(11) binding to horse spleen ferritin. Biochemistry, 1993, 32:1681~1687
10. 黄河清,张凤章,许良树等.细菌铁蛋白铁释放动力学研究.生物物理学报.1996.12(1):37~42
11. 张凤章,尹蓓,黄河清等,高柠橡酸盐对固氮酶铁钼辅基重组活性的影响、厦门大学学报(自然科学版).1996,35(1):100~106
12. 刘国诠等主编·生物工程下游技术·北京:化学工业出版社,1993
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备注/Memo

备注/Memo:
收稿日期:1996-4-30;接受日期:1996-10-18。
基金项目:国家自然科学基金,国家教委回国留学人员基金。
更新日期/Last Update: 1900-01-01