|本期目录/Table of Contents|

[1]丁诗华,李清漪.赤子爱胜蚓胆碱酯酶的纯化和性质[J].应用与环境生物学报,1997,3(03):246-251.
 Ding Shihua,Li Qingyi.PURIFICATION AND CHARACTERIZATION OF CHOLINESTERASE FROM EISENIA FOETIDA[J].Chinese Journal of Applied & Environmental Biology,1997,3(03):246-251.
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赤子爱胜蚓胆碱酯酶的纯化和性质()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
3卷
期数:
1997年03期
页码:
246-251
栏目:
论文
出版日期:
1997-08-25

文章信息/Info

Title:
PURIFICATION AND CHARACTERIZATION OF CHOLINESTERASE FROM EISENIA FOETIDA
作者:
丁诗华1 李清漪2
1. 西南农业大学水产科学系 重庆 630716;
2. 西南师范大学生命科学系 重庆 630715
Author(s):
Ding Shihua Li Qingyi
1. Dept Fisheries, SW Agricul Univ, Chongqing 630716;
2. Dept life Sci, SW Norm Univ, Chongqing 630715
关键词:
胆碱酯酶蚯蚓离子交换层析凝胶过滤
Keywords:
cholinesteraseearthwormion-exchange chromatographygel filtration
摘要:
赤子爱胜蚓的Triton X-100抽提液经PEG2000-磷酸钾缓冲液处理、离子交换层析及凝胶过滤后,获得电泳纯的胆碱酯酶.纯酶经鉴定为糖蛋白,凝胶过滤法和SDS-PAGE法测得其Mr分别为59000和58000.酸性氨基酸含量约占24%,碱性氨基酸含量约占12%,最大紫外吸收入λ=272um;酶作用最适温度θ=39℃,热稳定性较差;最适pH为7.8,在pH7.0~8.0酶较稳定.对碘化硫代乙酰胆碱的Km值为88μmol/L,有过量底物抑制现象.Mg2+、Ca2+和Mn2+对酶活性的影响较小,DFP则对该酶有强烈抑制作用.
Abstract:
Cholinesterase from the Triton X-100 extract of Eisenia foetida was purified to electrophoretic homogeneity by extraction with PEG 2 000/potassium phosphate buffer, ion-exchange chromatography and gel filtration. The purified enzyme was found to be a kind of glycoproteins. The Mr determined by gel filtration was 59000 and by SDS-PAGE was 58000. The contents of acidic amino acid and basic amino acid were about 24% and 12%, respctively. Ultraviolet spectrum showed a maximum absorption at 272 um. The enzyme with optimum temperature of 39℃ was unstable when heated. It exhibited optimum activity at pH 7.8 and kept stable between pH 7.8~8.0. The Michaelis constant for acetylthiocholine iodide was 88 μmol/L. The enzyme activity affected slightly by Mg2+,Ca2+ and Mn2+ was inhibited by excess substrate and strongly by DFP.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:1996-7-29;接受日期:1996-10-16。
更新日期/Last Update: 1900-01-01