|本期目录/Table of Contents|

[1]曾庆平,刘叔文,郭勇,等.甲基紫精对植物细胞若干生理生化指标的影响[J].应用与环境生物学报,1996,2(04):405-407.
 Zeng Qingping,Liu Suwen,Guo Yong,et al.EFFECT OF METHYL VIOLOGEN ON SEVERAL PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERS OF PLANT CELLS[J].Chinese Journal of Applied & Environmental Biology,1996,2(04):405-407.
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甲基紫精对植物细胞若干生理生化指标的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
2卷
期数:
1996年04期
页码:
405-407
栏目:
论文
出版日期:
1996-11-25

文章信息/Info

Title:
EFFECT OF METHYL VIOLOGEN ON SEVERAL PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERS OF PLANT CELLS
作者:
曾庆平1 刘叔文1 郭勇1 郑学勤2
1. 华南理工大学生物工程系, 广州 510641;
2. 中国热带农业科学院热带作物生物技术国家重点实验室, 儋州 571737
Author(s):
Zeng Qingping Liu Suwen Guo Yong Zheng Xueqing
1. Dept of Biotech, South China University of Technology, Guangzhou 510641;
2. National Key Lab of Biotech for Tropical Crops, Chinese Academy of Tropical Agriculture, Danzhou 571737
关键词:
甲基紫精玫瑰茄(Hibiscus sabdariffa)SOD诱导抗性
Keywords:
methyl viologenrossel (Hibiscus sabdarifa)SODinductionresistance
摘要:
联毗咤类衍生物甲基紫精(俗名百草枯,以下简称MV)是一种触杀灭生性除草剂,其作用机理是诱发细胞产生过量的超氧化物自由基,从而导致多种有害的细胞学效应[1].超氧化物歧化酶(简称SOD)具有消除过量氧自由基O2的能力,SOD活力越高,对MV的抗性越强[2]研究证明,SOD是一类受发育特异性调节和环境诱导表达的酶[9],可以受各种“氧化逆境”的诱导,如强光、低温、缺氧、干旱、淹水等[4].为了定量研究MV与植物细胞若干生理生化指标的相关性,我们从SOD活力、生物膜通透性以及叶绿素降解程度的动态变化方面,考察了低浓度MV在诱导SOD基因高效表达中的作用以及植物抗MV的分子机制.
Abstract:
After dark induction for 16h and 24h with 1 μmol/L of methyl viologen,the SOD activities and specific activities of cultured rossel (Hibiscus sabdariffa) cells were increased by 74.8%,324.2%and 69.99%,295.73%,respectively,compared with CK.After contacting with high concentration,1000 μmol/L of methyl viologen,the electrolyte leakage Percentage of those cells pretreated with 1μmol/L of methyl viologen were decreased by 4 8% and at a very significant level (P≤0.01)with difference.The results suggested that the pretreatment of lower concentration of methyl viologen could provide an oxidative stress and induce overexpression of SOD genes,and thus could decrease or eliminate cell damage, such as biomembrane leakage and chlorophyll breakdown,resulted from the free radical O2.

参考文献/References:

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[9] 李宝胜,曾庆平.植物生物技术原理与方法.长沙:湖南科学技术出版社,1990
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备注/Memo

备注/Memo:
收稿日期:1995-10-16;接受日期:1996-4-3。
基金项目:华南生物科学与技术中心资助项目。
更新日期/Last Update: 1900-01-01