|本期目录/Table of Contents|

[1]沈建华,W. Kesler.N,N-二甲基甲酰胺在血液中代谢物S-(N-甲基氨基甲酰)谷胱甘肽的气质联用方法[J].应用与环境生物学报,1996,2(04):376-381.
　Shen Jianhua,W. Kesler.A GC-MS METHOD FOR QUANTITATING S-(N-METHYLCARBAMYOL)GLUTATHIONE,A METABOLITE OF N,N-DIMETHYLFORMAMIDE IN BLOOD[J].Chinese Journal of Applied & Environmental Biology,1996,2(04):376-381.
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N,N-二甲基甲酰胺在血液中代谢物S-(N-甲基氨基甲酰)谷胱甘肽的气质联用方法()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
2卷
期数:
1996年04期
页码:
376-381
栏目:
论文
出版日期:
1996-11-25

文章信息/Info

Title:
A GC-MS METHOD FOR QUANTITATING S-(N-METHYLCARBAMYOL)GLUTATHIONE,A METABOLITE OF N,N-DIMETHYLFORMAMIDE IN BLOOD
作者:
沈建华1;  W. Kesler2
1. 中国科学院上海昆虫所生化毒理研究室, 上海 200025;
2. 德国国家环境与健康研究中心毒理研究所, 慕尼黑 德国
Author(s):
Shen Jianhua;  W. Kesler
1. Department of Biochemistry and Toxicology, Shanghai Institute of Entomology Academia Sinien Shanghai 200025;
2. National Research Center for Environment and Healthe, Institute of Toxicology, Munich, F.R.G.
关键词:
N; N-二甲基甲酰胺; 血液; 硫酯; 气质联用
Keywords:
N; N-dimethylformamide; blood; thioesters; GC-MS
摘要:
根据先将SMG转化为乙基、N-甲基氨基酸盐(EMC),然后以气相色谱-质谱联用手段测定所产生的EMC的原理,发展出一种定量检测大鼠血清中SMG及其他N-甲基氨基甲酸硫酯类物质的分析方法。方法采用人工合成的SMG和EMC进行标定,每次测定需要样品量为0.5mL,方法的合理检出极限为c(SMG)=10μmol/L.
Abstract:
N,N-dimethylformamide (DMF)is widely used as an orsanic solvent in different chemical processes.The toxicology of DMF is related to its metabolites.The results published in recent years suggest the possible existence of S-(N-methylcarbemoyl) glutathione (SAG) and S-(N-methylcarbamoyl) cystein (SMC) as inter mediate metabolites in mammalians.On the basis of conversion of SMG into ethyl N-methylcarbamate(EMC) that was measured on the OC-MS, a method for quantitatins SAG and other N-methylcarbamoyl thioesters in rat blood with the detection limits of 10 μmole SMG in 1 liter biood plasma was developed.The method was valianted with synthetic SAG and EMC.It took 0.5 mL of bloob plasma for each assay.

参考文献/References:

[1] WHO.Dimethylfomarmide.Emvironmental Health Criteria 114,Ceneva:World Health Organization,1990
[2] IARC.Dimethylformamide.In:Sonae organic solrents,resin ntanonrers mul relnfed contponnrls,pigments and occupnironal erposures in man ufacture and panting,LARC monograph on the evzalution of carcinogcric risks to humans,Vo1.47.Lyon:lnterrnational Agency for Research on Cancer.1989.171-197
[3] Barnes JR,Ranta KR.The metabolism of dimethylformamide and dimethylacetamide.Torxcol Appl plurmal.1972,23:271-276
[4] Mraz J,Gescher A,Grass HJ et n1. New finding in the metabolism of N,N-dimethylformamide-consequences for evaluation of occupntional risk.Sci Total Environ.1991,101:131-134
[5] Mraz J,Jheeta P,Gescher A et al.Tnvestigation of the mechanistic basis of toxicology,metabolism of N,N-dimethylformamiJe ang its deuterated isotopmers by cytochrome P.150 2E1.Chem Res Tasicol.1993,6:197-207
[6] Pearson PG,Slatter JG,Rashed MS et nl. S-(N-methylformamyol)glntathione:a reactive S-linked metabolite of methylLrocynate.Biochem Biophys Res Commun.1990,186:215-250
[7] Han D-H,Pearson PG,Baillie TA et al.Chemical synthesis and cytotoxic properties of N-xcetylcarbamic acid thioesters.metatbolites of hepatotoxic formamides.Chem Res Toxicul.1990,3:118-124
[8] Guest I,Ballie TA,Varma DR.Toxicology of the methylisocynate metalwlite S-(N-methylcarbamyol)GSH on mouse embryos in culture.Teratology.1992,46:61-67

相似文献/References:

[1]沈建华,KESLER,W..大鼠全身曝露N,N－二甲基甲酰胺蒸汽后血液中N－甲基氨基甲酸硫酯动态研究[J].应用与环境生物学报,1998,4(01):32.
　SHEN Jianhua,et al..Kinetic study on Nmethylformayol thioesters in rat blood after wholebody exposure to N,Ndimethylformamide vapors[J].Chinese Journal of Applied & Environmental Biology,1998,4(04):32.

备注/Memo

备注/Memo:
收稿日期:1996-2-5;接受日期:1996-5-23。
更新日期/Last Update: 1900-01-01