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[1]鲍锦库,周红,曾仲奎,等.岩豆凝集素的纯化及其分子残基的化学修饰和活性关系[J].应用与环境生物学报,1996,2(03):214-219.
 Bao Jinku,Zhou Hong,Zeng Zhongkui,et al.STUDY ON THE RELATION BETWEEN RESIDUE MODIFICATION AND BIOLOGICAL ACTIVITY OF MILLETTIA DIELSIANA HARMS. LECTIN[J].Chinese Journal of Applied & Environmental Biology,1996,2(03):214-219.
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岩豆凝集素的纯化及其分子残基的化学修饰和活性关系()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
2卷
期数:
1996年03期
页码:
214-219
栏目:
论文
出版日期:
1996-08-25

文章信息/Info

Title:
STUDY ON THE RELATION BETWEEN RESIDUE MODIFICATION AND BIOLOGICAL ACTIVITY OF MILLETTIA DIELSIANA HARMS. LECTIN
作者:
鲍锦库 周红 曾仲奎 杨莉
四川联合大学生物系生物化学教研室 成都 610064
Author(s):
Bao Jinku Zhou Hong Zeng Zhongkui Yang Li
Dept Biol, Sichuan Univ Univ, Chengdu 610064
关键词:
岩豆凝集素分子修饰糖蛋白
Keywords:
Millettia dielsiana Harms.lectinmolecular modificationglycoprotein
摘要:
岩豆种子经侵取、硫酸铵分级、DEAE-纤维素离子交换和SephadexG-100分子筛层析,获得FPLC上单一的凝集素样品,对此纯化的凝集素样品用N-溴代丁二酰亚胺、N-乙基顺丁烯二酰亚胺、二流苏糖醇,碘乙酸和对氯汞苯甲酸修饰其分子内的色氨酸和巯基。经计算,该凝集素分子内含有4个色氨酸和24个流基,当色氨酸被修饰后其凝集活性全部丧失,而巯基被修饰后对其凝血活性无任何影响,表明巯基与凝集素分子的凝血活性无关,而色氨酸则是凝集活性所必需的基团。
Abstract:
Millettia dielsiana Harms.Lectin (MDL)was purified from the seeds of Millettia dielsiana Harmesx Diels by extraction, fraction with (NH4)2SO4, DEAE-Cellulose ion-exchange chromatography and followed by gel filtration of Sephadex G- 100.The purified MDL showed single protein peak on FPLC,mwhfication disulfide groups(-SH)and Tryptophan(Trp)in MDL.The result showed that MDL did not need the -SH to deep its activity,but Trp was essential for MDL to keep activity.There were 4 Trp and 24 -SH in MDL molecules.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:1995-12-12;接受日期:1996-3-25。
基金项目:国家自然科学基金
更新日期/Last Update: 1900-01-01