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[1]张耀洲,龚柏伟.芜菁花叶病毒杭州株P1基因的克隆和在大肠杆菌中的表达[J].应用与环境生物学报,1996,2(02):141-146.
 Zhang Yaozhou,Gong Bowei.CLONING AND EXPRESSION OF THE P1 GENE OF TURNIP MOSAIC VIRUS HANGZHOU ISOLATE I[J].Chinese Journal of Applied & Environmental Biology,1996,2(02):141-146.
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芜菁花叶病毒杭州株P1基因的克隆和在大肠杆菌中的表达()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
2卷
期数:
1996年02期
页码:
141-146
栏目:
论文
出版日期:
1996-05-25

文章信息/Info

Title:
CLONING AND EXPRESSION OF THE P1 GENE OF TURNIP MOSAIC VIRUS HANGZHOU ISOLATE I
作者:
张耀洲1 龚柏伟2
1. 浙江农业大学生物化学研究所, 杭州310029;
2. 中国科学院上海细胞研究所, 上海200031
Author(s):
Zhang Yaozhou Gong Bowei
1. Institlte of Biochemistry, Zhejiang Agricultural University, Hangzhou 310029;
2. Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031
关键词:
芜菁花叶病毒P1基因大肠杆菌表达
Keywords:
Turnip mosaic virusP1 geneE. coli expression
摘要:
通过反转录PCR,克隆得到1.2kb的TuMVH1的P1片段,并在大肠杆菌表达载体pGEMEX-1中得以表达,表达产物为可融性融合蛋白,Mr≈6×104
Abstract:
The turnip mosaic virus Hangzhou isolate I(TuMV H1),wnich infected Brassica naprs more seriously than other TuMV isolates,was isolated in Hangzhou.The P1 gene of TuMV H1 was cloned by reverse transcription PCR and was inserted into pGEMEX-1 fusion expression vector.A fusion protein containing the P1 protein that is Mr≈6×104 was expressed in E. coli.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:1994-12-8;接受日期:1995-3-19。
基金项目:国家自然科学基金;浙江省自然科学基金。
更新日期/Last Update: 1900-01-01