|本期目录/Table of Contents|

[1]郭永灿,王振中,张友梅,等.重金属对蚯蚓的毒性毒理研究[J].应用与环境生物学报,1996,2(02):132-140.
 Guo Yongcan,Wang Zhenzhong,Zhang Youmei,et al.STUDIES ON TOXICITY AND TOXICOLOGY OF HEAVY METALS TO EARTHWORMS IN POLLUTED SOILS[J].Chinese Journal of Applied & Environmental Biology,1996,2(02):132-140.
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重金属对蚯蚓的毒性毒理研究()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
2卷
期数:
1996年02期
页码:
132-140
栏目:
论文
出版日期:
1996-05-25

文章信息/Info

Title:
STUDIES ON TOXICITY AND TOXICOLOGY OF HEAVY METALS TO EARTHWORMS IN POLLUTED SOILS
作者:
郭永灿2 王振中2 张友梅1 赖勤1 颜亨梅1 夏卫生2 邓继福3
1. 湖南师范大学生物系, 长沙410006;
2. 国土资源及环境科学系, 长沙410006;
3. 湖南省株洲市环境保护科研所, 株洲4125000
Author(s):
Guo Yongcan Wang Zhenzhong Zhang Youmei Lai Qin Yan Hengmei Xia Weisheng Deng Jifu
1. Department of Biology, Hunan Normal University, Changsha 410006;
2. Department of Resource and Environment, Hunan Normal University, Changsha 410006;
3. Zhuzhou Institute of Environmental Protection Science, Zhuzhou 412000
关键词:
毒理学重金属污染蚯蚓毒性显微结构超微结构
Keywords:
toxicitytoxicologyheavy metal pollutionearthwormmicrostructuneultrastructure
摘要:
通过室内模拟实验以及对株洲市重金属污染区农田土壤中采样,进行了重金属对蚯蚓的毒性毒理研究。毒性实验表明,各重金属元素48h的LD50分别为:Cd 1000mg/kg,Pb 812mg/kg,Cu 633mg/kg,Hg 304mg/kg,Zn 528mg/kg,Cr 428mg/kg.对蚯蚓毒理研究证明,重金属对蚯蚓酯酶同工酶有抑活作用,而重金属对蚯蚓过氧化物酶同工酶有激活作用。在光镜下观察,可见重金属污染可引起蚯蚓的体表溃疡及产生肿瘤,可使胃肠道粘膜层出血、背血管肿胀,同时可引起胃肠道粘膜上皮细胞产生萎缩或溃疡灶。蚯蚓特有的黄色细胞团污染程度不同而显示出消长规律。在电镜下可见到在重污染区的蚯蚓胃肠道粘膜上皮细胞中的rER肿胀、高尔基体膨大,线粒体嵴消失,甚至空泡化或解体。另可见细胞核膜间隙肿胀、断裂、核质外溢,胞质自溶。蚯蚓以上病变的大小是随土壤重金属污染区的污染级别而定;各病理变化指标均能定性地反映土壤重金属污染状况。
Abstract:
The results from the tests of heavy metal toxicity to the earthworms collected from the Polluted soils indicated that the various heavy metals LD50(mg/kg)after treated for 48h were cd 1000 mg/kg,Pb 707mg/kg,Cu 633 mg/kg,Hg 304 mg/kg,Zn 566 mg/kg,and Cr 450 mg/kg respectively.The toxicological analyses indicated that the activity of esterase isozymes of the earthworms was inhibited by the heavy metals,but their catalase isozymes were activated. Under light microscope,it could be observed that the heavy metal pollution might cause ulcer or tumour on the body surface of earthworms,blood letting and epithelial cells shrinking or atrophying on their intestinal mucosa and dorsal blood vessels swelling.Under electron microscope,it could be seen that the rER,Golgi cisternae and mitochondrion swelling in the mucosa cells of the earthworms from the seriously polluted soils,and even the vesiculation appeared or lysis took place and cristae disappered.It could also be seen that in the body of earthworm the perinuclear cisterna swelled)nuclear envelope broke,nucleoplasm flowed out and complasm autolysed. These pathologies changed with different degrees of heavy metal pollution.Each of pethological indiCators could qualitatively responds to heavymetal pollution in soils.

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备注/Memo

备注/Memo:
收稿日期:1995-3-2;接受日期:1995-9-7。
基金项目:国家自然科学基金。
更新日期/Last Update: 1900-01-01