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[1]麻密,周达锋,许家喜,等.细胞分裂素基因(T-cyt)在转基因烟草中的表达及其对生长发育的影响[J].应用与环境生物学报,1996,2(01):1-7.
 Ma Mi,Zhou Dafeng,Xu Jiaxi,et al.THE EXPRESSION OF T-cyt GENE IN TRANSGENIC TOBACCO AND REGULATION FOR PLANT DEVEOLPMENT[J].Chinese Journal of Applied & Environmental Biology,1996,2(01):1-7.
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细胞分裂素基因(T-cyt)在转基因烟草中的表达及其对生长发育的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
2卷
期数:
1996年01期
页码:
1-7
栏目:
论文
出版日期:
1996-02-25

文章信息/Info

Title:
THE EXPRESSION OF T-cyt GENE IN TRANSGENIC TOBACCO AND REGULATION FOR PLANT DEVEOLPMENT
作者:
麻密1 周达锋1 许家喜2 匡廷云1 汤佩松1 林忠平1
1. 中国科学院植物研究所 北京 100044;
2. 北京大学化学系 北京 100871
Author(s):
Ma Mi Zhou Dafeng Xu Jiaxi Kuang Tingyun Tang Peisong Lin Zhongping
1. Institute of Botany, Academia Sinica, Beijing 100044;
2. Department of Chemistry, Peking University, Beijing 100871
关键词:
细胞分裂素T-cyt基因启动子基因表达转基因烟草
Keywords:
cytokininT-cyt genepromotergene expressiontransgenic tobacco
摘要:
将编码细胞分裂素合成关键酶──异戊烯基转移酶的T-cyt基因分别置于CaMV35S启动子,rbcS启动子和T-cyt基因自身启动子的调控下,构建成不同的嵌合质粒用于转化烟草,通过PCR检测,Southern杂交和NPTⅡ的酶活检测对获得的转基因烟草进行了鉴定。Northern分析表明:CaMV35S启动子驱动下的T-cyt基因在转基因烟草的根、茎、叶中均有mRNA的积累;rbcS启动子指导下T-cyt基因在叶中转录较强,茎中次之,在根中几乎未表达。以根据抗原决定簇进行人工合成的复合抗原免疫家兔得到异戊烯基转移酶抗体,ELISA结果表明转基因烟草根中异戊烯基转移酶含量较高。T-cyt基因的表达对转基因烟草的生长发育有明显影响:其叶绿素a、b含量明显增加,叶衰老迟缓;顶端优势受到抑制,侧芽生长旺盛;与对照相比,其根系不发达。
Abstract:
The T-cyt gene Coding the key enzyme of cytokinin biosynthesis was placed under control of CaMV 35S,rbc S and T-cyt native promoters respectively.The chimeric plasmids were transferred separatedly into Nicotiana tubacum. The transgenic plants were confirmed by PCR DNA amplification,Southern hybridization and NPT Ⅱ detection.The results of Northern analysis showed that T-cyt gene could express in leaves,stems and roots of tobacco with transgene of caMV 35S/T-cyt. The transcript level of rbc S/T-cyt gene activities in leaves was higher than that of stems and little transcript activity was found in roots of those plants. ELISA indicated that the roots of transgenic tobacco contained more isopenteryl transferase. Moreover, a series of morphological and physiological changal, such as promotion of axillary bud growth, inhibition of root srowth, promotion of chlorophyll synthesis and delay of leaf senescence,were observed.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:1995-7-10;接受日期:1995-8-21。
基金项目:国家自然科学基金。
更新日期/Last Update: 1900-01-01