|本期目录/Table of Contents|

[1]叶彦,缪树华.长期继代培养马铃薯愈伤组织的植株再生[J].应用与环境生物学报,1995,1(01):26-33.
 Ye Yan,Miao Shuhua.PLANT REGENERATION FROM LONG-TERM SUBCULTURED CALLI OF POTATO[J].Chinese Journal of Applied & Environmental Biology,1995,1(01):26-33.
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长期继代培养马铃薯愈伤组织的植株再生()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
1卷
期数:
1995年01期
页码:
26-33
栏目:
研究论文
出版日期:
1995-02-25

文章信息/Info

Title:
PLANT REGENERATION FROM LONG-TERM SUBCULTURED CALLI OF POTATO
作者:
叶彦 缪树华
中国科学院成都生物研究所, 成都 610041
Author(s):
Ye Yan Miao Shuhua
Chengdu Institute of Biology, Academia Sinica, Chengdu 610041
关键词:
马铃薯愈伤组织继代培养植株再生
Keywords:
potatocallussubcultureplant regeneration
摘要:
对影响马铃薯愈伤组织发生、生长和长期继代培养的愈伤组织再生植株的几种因素进行了研究.马铃薯茎段的愈伤组织发生频率高于叶片的愈伤组织发生频率.Dicamba诱导茎段和叶片的愈伤组织发生频率在90%以上;而2,4-D的诱导效果不佳并诱发外植体生根.再生培养基中加入0.5g/L的酶解酪蛋白是必需的;蔗糖的浓度以2%-4%为宜;附加ZT2.0mg/L、GA0.1mg/L可使长期继代的愈伤组织的植株再生频率达80%.三个品种的马铃薯愈伤组织只有一种可再生植株,基因型差异明显.此外,还从再生植株的茎段诱导了次级愈伤组织并再生了植株.
Abstract:
The factors effecting callus initiation and growth, as well as plant regeneration from long-term cultured calli of potato(Solanum tuberosum L.) were studied. The frequency of callus initiation from stem segments was higher than that from leaf discs. The frequency of callus initiation by Dicamba was about 90% from stem segments and leaf discs, but that by 2, 4-D was lower, and roots were induced on the explants. It was necessary to supply casein hydrolasate at 0.5 g/L in the regeneration medium. The suitable concentration of sucrose was 2% -4%. The regeneration frequency of long-term cultured calli could reach 80% when ZT 2.0 mg/L and GA0.1mg/L were supplied in the regeneration medium. Of the three tested genotypes.only one could reproduce plants. Furtheremore,secondary calli were also induced and could reproduce plants.

参考文献/References:

[1] 孙敬三,陈维论主编.植物生物技术和作物改良.见:李耿光.茄属植物原生质体培养和融合.北京:中国科学技术出版社,1990.7.242-247
[2] Magro J. Sur la tuberation de la pomme de terre. Comp Rcwl Soc Rcol. 1938.127:739
[3] Montezuma-de-carvalho J. Isolation of a callus from potato roots. Bot Sor Rrol. 1976.50:143-163
[4] 韩善华.郑国错.马铃薯(Solmenm Cnbrroawn L.)幼茎(芽)愈伤组织的诱导和植株再生.实验生物学报.1982.15:111-115
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[6] Webb K J.Osifo E O. Hen.shaw G G. Shoot regeneration from leaflet discs of six cultivars of potato (sulnanm tuberasum Subsp.tuberosum). Plant Scieace letters. 1983.30:1-8
[7] Nagayoshi Oshima.Livingston C H.In-mtro culture of potato tissue Ⅰ. American Polate.Junranl. 1963.40:9-16
[8] Shaw R. Potato tuber callus-vaditation as biochemical tool. Plant Phystol 1976.58: 461-467
[9] Simmonds N W. Observation on potato callus and adventitious shoot formation. American Potato Journal. 1964,4: 129-136
[10] Cappadocia M,Cheng D S K. Ludlum-Simonette R.Plant regeneration from to pulto culture of anther of Sulinam charaterse. Bitt. and interspecific diploid hybrids S. tuberasum L. S, chacoense Bitt. Theor Appl Geact 1984.69:139-143
[11] Radke S.Grun P. lsolation,culture and regeneration of leaf masophyll protoplasts of selected clones of Solanum. Potato Research.1986,29:451-462
[12] Shue-Lock L. Regeneration of plantlets from single cells in potatoes. American,Patato Jouranl. 1977, 54:575-581
[13] Lillo C. Effect of media components and environmental factors on shoot formation from protoplast-derived calli.Solanum tuberasum.Plant Cell,Tiscue and Orgrta Culture. 1989,19.103-111
[14] Shepard J F. Cultivar dependent cultural refinements in potato protoplast regeneration. Plant Science Letters,1982, 26:127-132
[15] Creissen G P,Karp A. Karyotypic changes in potato plants regenerated from protoplasts. Plant Cell,Tissue and Orgen cultrue. 1985.4:171-182

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备注/Memo

备注/Memo:
收稿日期:1994-7-18;接受日期:1994-11-21。
更新日期/Last Update: 1900-01-01