|本期目录/Table of Contents|

[1]付竞峰,秦婷婷,杜林方.暗处理对油菜子叶类囊体膜色素组成和荧光性质的影响[J].应用与环境生物学报,2008,14(01):24-031.
 FU Jingfeng,et al..Effect of Dark Treatment on Fluorescence Properties of Cytoledon Thylakoid Membranes of Brassica napus L.[J].Chinese Journal of Applied & Environmental Biology,2008,14(01):24-031.
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暗处理对油菜子叶类囊体膜色素组成和荧光性质的影响()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
14卷
期数:
2008年01期
页码:
24-031
栏目:
综述
出版日期:
2008-01-15

文章信息/Info

Title:
Effect of Dark Treatment on Fluorescence Properties of Cytoledon Thylakoid Membranes of Brassica napus L.
作者:
付竞峰秦婷婷杜林方
(四川大学生命科学学院/生物资源与生态环境教育部重点实验室成都610064)
Author(s):
FU Jingfeng et al.
(Key Laboratory of Bioresources and Ecoenvironment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, China)
关键词:
关键词油菜子叶类囊体膜暗处理光谱性质
Keywords:
Keywords Brassica napus L. cotyledon thylakoid membrane dark treatment spectra property
摘要:
摘要以蜀杂10号油菜为材料,播种7 d后分别置于自然光照条件和黑暗环境中进行暗处理,未处理油菜在d 5、 d 7、 d 14、 d 17、 d 21、d 24和d 27时收集子叶,暗处理油菜于播种d 8、 d 10、 d 12和d 14时收集子叶. 制备油菜子叶类囊体膜,分析色素含量,并进行类囊体膜丙酮抽提液室温吸收光谱、叶绿素荧光发射和激发光谱以及蛋白质内源荧光光谱的分析. 结果显示:与未处理油菜相比,暗处理引起油菜子叶类囊体膜光合色素Chl a和Chl b含量急剧减少,Chl a/b比值和叶绿素/类胡萝卜素(Chl/Car)比值持续下降,类囊体膜对叶绿素的捕光能力和受激发能力降低,类囊体膜蛋白质内源荧光强度降低,以及色素蛋白复合物降解. 上述结果表明,暗处理诱导油菜子叶快速进入衰老阶段,类囊体膜蛋白组成和色素的光合功能均发生了明显的变化. 图10 参23
Abstract:
Abstract Shuza10 rapeseed (Brassica napus L.) was grown under natural and dark conditions 7 days after sowing. The cotyledons of untreated rapeseed were harvested on day 5, 7, 14, 17, 21, 24 and 27, and those of the dark treated on day 8, 10, 12, and 14. Thylakoid membranes were prepared from the rapeseed cotyledons of the two treatments, respectively. A comparative study on their pigment composition, absorption spectra at room temperature, chlorophyll emission and excitation fluorescence spectra and protein flourescence spectra was conducted. Compared with the untreated rapeseed, the cotyledon thylakoid membranes treated in dark had much less Chl (Chl a and Chl b), and a lower Chl a/b ratio and chlorophyll/carbtenoid (Chl/Car) ratio. Furthermore, the chlorophyll fluorescence spectra indicated that its capacities of harvesting light and being excited were lower after dark treatment. In addition, the protein composition of cotyledon thylakoid membranes also changed in the dark treatment, suggested by significant difference in protein influorescence. The data indicated that dark treatment induced senescence of rapeseed cotyledon. Fig 10, Ref 23

相似文献/References:

[1]周冀明,卫志明,刘世贵,等.诸葛菜下胚轴和子叶原生质体培养[J].应用与环境生物学报,1996,2(01):8.
 Zhou Jiming,Wei Zhiming,Liu Shigui,et al.CULTURE OF PROTOPLASTS FROM HYPOCOTYLS AND COTYLEDONS OF ORYCHOPHEAGMUS VIOLACEUS[J].Chinese Journal of Applied & Environmental Biology,1996,2(01):8.

更新日期/Last Update: 2008-03-05