|本期目录/Table of Contents|

[1]史公军,侯喜林,王彦华.白菜叶绿体DNA快速提取及分析[J].应用与环境生物学报,2005,11(03):283-285.
 SHI Gongjun,et al..Extraction and analysis of chloroplast DNA in nonheading Chinese cabbage[J].Chinese Journal of Applied & Environmental Biology,2005,11(03):283-285.
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白菜叶绿体DNA快速提取及分析()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
11卷
期数:
2005年03期
页码:
283-285
栏目:
综述
出版日期:
2005-06-25

文章信息/Info

Title:
Extraction and analysis of chloroplast DNA in nonheading Chinese cabbage
作者:
史公军侯喜林王彦华
(南京农业大学1作物遗传与种质创新国家重点实验室,2园艺学院南京210095)
Author(s):
SHI Gongjunet al.
(1National Key Laboratory of Crop Genetics & Germplasm Enhancement, 2 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
关键词 白菜 SDS蛋白酶法 叶绿体DNA 提取
Keywords:
Keywords nonheading Chinese cabbage SDSproteinase method cpDNA extraction
摘要:
摘要 采用SDS-蛋白酶法提取了白菜的叶绿体DNA. 琼脂糖电泳及紫外分光光度分析表明,所提取的叶绿体DNA质量高,除cpDNA主环外,在胞质雄性不育新种质及原不育系中还存在约1 375 bp和831 bp的两条质粒分子.用所提取的叶绿体DNA作为模板进行RAPD扩增,获得了清晰的扩增图谱.该方法与传统的提取方法相比省时、高效、无污染. 图2 表1 参14
Abstract:
Abstract Chloroplast DNA was extracted from young seedlings of nonheading Chinese cabbage by SDS proteinase method. Its quality was evaluated by agrose gel electrophoresis and UV spectrophotometer. Except the main cpDNA band, two minor particle molecules about 1 375 bp and 831 bp appeared in the profile of the original and new CMS line. RAPD analysis was made with the cpDNA as template and clear amplification profile was obtained. This method was proved to be more efficient, more timesaving and less pollution than traditional methods. Fig 2, Tab 1, Ref 14
更新日期/Last Update: 2005-06-24