|本期目录/Table of Contents|

[1]冯霞,殷幼平,王中康.现代分子生物学技术在动物肠道微生物多样性研究中的应用[J].应用与环境生物学报,2005,11(03):381-387.
 FENG Xia,et al..Application of molecular biological techniques in research of microbial diversity in animal intestines[J].Chinese Journal of Applied & Environmental Biology,2005,11(03):381-387.
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现代分子生物学技术在动物肠道微生物多样性研究中的应用()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
11卷
期数:
2005年03期
页码:
381-387
栏目:
综述
出版日期:
2005-06-25

文章信息/Info

Title:
Application of molecular biological techniques in research of microbial diversity in animal intestines
作者:
冯霞殷幼平王中康
(重庆大学生物工程学院 教育部生物力学与组织工程重点实验室 重庆 400044)
Author(s):
FENG Xiaet al.
(Key Lab for Biomechanics & Tissue Engineering of the Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China)
关键词:
关键词 16S rRNA 16S23S rDNA间区 遗传指纹图谱技术 肠道微生物多样性
Keywords:
Keywords 16S rRNA 16S23S rDNA ISR genetic fingerprint profile technique intestine microbial diversity
摘要:
摘要 现代分子生物技术的蓬勃发展解决了不可培养微生物研究的难题,使得肠道微生物的研究进入一个新的阶段.本文主要介绍了基于16S rRNA的分子分析技术,包括DGGE (变性梯度凝胶电泳),TGGE (温度梯度凝胶电泳),SSCP (单链构象多态性),RFLP (限制性片段长度多态性)等肠道微生物研究工作中常用的分子生物学技术方法、适用范围及可能的发展方向,为动物肠道微生物区系的进一步研究及开发应用提供帮助. 表1 参42
Abstract:
Abstract Molecular biological techniques were applied in microecological research and resolved lots of difficulties in studying the microorganisms, which could not be cultured. So the research of intestine microbes gained a new phase. This paper presents a minireview on the molecular ecological techniques used for microbial diversity research in animal guts based on 16S rRNA, as well as 16S23S rDNA Intergenic Spacer Region, RAPD, LMW RNA, fluorescence in situ hybridization, including a series of genetic fingerprint profile techniques, such as DGGE (denaturing gradient gel electrophoresis), TGGE (temperature gradient gel electrophoresis), SSCP (singlestranded conformation polymorphism ) and RFLP (restriction fragment length polymorphisms). Tab 1, Ref 42
更新日期/Last Update: 2005-06-24