|本期目录/Table of Contents|

[1]刘昀,李凤霞,郑易之,等.香花槐组培苗快繁体系的建立及工厂化育苗的主要影响因素[J].应用与环境生物学报,2004,10(02):162-165.
 LIU Yun,et al..Clonal propagation of Robinia cv. Idaho and main factors affecting its largescale production[J].Chinese Journal of Applied & Environmental Biology,2004,10(02):162-165.
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香花槐组培苗快繁体系的建立及工厂化育苗的主要影响因素()
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
10卷
期数:
2004年02期
页码:
162-165
栏目:
综述
出版日期:
2004-04-25

文章信息/Info

Title:
Clonal propagation of Robinia cv. Idaho and main factors affecting its largescale production
作者:
刘昀李凤霞郑易之郝东时高天舜朱冬梅何孟元
(2东北师大林木生物工程有限责任公司长春130022) (3深圳大学生命科学学院广东深圳518060)
Author(s):
LIU Yunet al.
(1School of Life Science, Northeast Normal University,Changchun130024, China) (2NENU Seedling Bioengineering Co. Ltd., Changchun130022, China) (3College of Life Science, Shenzhen University, Shenzhen, Guangdong518060, China)
关键词:
关键词 香花槐 组织培养 工厂化快繁 超度含水态苗
Keywords:
Keywords Robinia cv. Idaho tissue culture largescale propagation hyperhydricity
摘要:
摘要 建立了香花槐组培苗快繁无性系.在改良MS培养基中添加ρ(6BA)/mg L-1=0.35~0.5、ρ(NAA)/mg L-1=0.05~0.08, ρ(GA3)/mg L-1=0.07~0.1作为生长培养基.在丛生芽诱导培养基中,ρ(6BA)/mg L-1=0.8~1.4,其余成分同生长培养基.两种培养方法同时使用,保证了组培苗繁殖系数为5左右.生根培养基中大量元素为MS基本培养基的1/4, ρ(IBA)/mg L-1=0.1~0.5、ρ(IAA)/mg L-1=1.7~2.5,香花槐组培苗的生根率为90%.炼苗后组培苗的移栽成活率为85%,且植株表型未见变异.将MS基本培养基中硝酸钾的含量由1.9 g/L提高至2.199~2.931 g/L,可满足每代香花槐试管苗生长25~35 d期间对钾的需求.将培养基中6BA的含量降至0.4 mg/L,并采用合适的组培容器,在连续培养2、3代后,可将超度含水态苗的发生频率控制在10% 以下.在6 mo内生产100万株香花槐组培移栽苗. 图1 表1 参16
Abstract:
Abstract Clonal propagation of Robinia cv. Idaho plantlets was established. MS medium with ρ(6 BA)/mg L-1 =0.35~0.5,ρ(NAA)/mg L-1=0.05~0.08 and ρ(GA3)/mg L-1=0.07~0.1 was used for its growth medium. In inducing medium, ρ(6BA)/mg L-1=0.8~1.4 was used for inducing cluster buds. Two media were used so as to keep regenerating ratio of plantlets about 5. Each of more than 90% plantlets regenerated 2~4 roots when they were cultured in the 1/4 MS medium with ρ(IBA)/mg L-1=0.1~0.5 and ρ(IAA)/mg L-1=1.7~2.5. Over 85% of them survived after transplanted in greenhouse, and no obvious variation in phenotype was observed. The content of KNO3 in the MS medium increased from 1.9 g/L up to 2.199~2.931 g/L to meet the need of plantlets that had been growing in medium for 25 ~35 days. The ratio of hyperhydric plantlets was reduced to 10% by substracting the content of 6 BA to 0.4 mg/L in medium and by using the containers which could exchange air easily. And one million plantlets were produced in only 6 months. Fig 1, Tab 1, Ref 16

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更新日期/Last Update: 2004-04-22