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[1]蒲勤华,黄维藻,许春燕,等.番茄微管结合蛋白EB1调控盐胁迫应答[J].应用与环境生物学报,2018,24(06):1359-1364.[doi:10.19675/j.cnki.1006-687x.2018.03007]
 PU Qinhua,et al..Tomato microtubule-associated protein end-binding protein 1 (EB1) mediates tolerance to salt stress[J].Chinese Journal of Applied & Environmental Biology,2018,24(06):1359-1364.[doi:10.19675/j.cnki.1006-687x.2018.03007]
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番茄微管结合蛋白EB1调控盐胁迫应答
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《应用与环境生物学报》[ISSN:1006-687X/CN:51-1482/Q]

卷:
24卷
期数:
2018年06期
页码:
1359-1364
栏目:
研究论文
出版日期:
2018-12-25

文章信息/Info

Title:
Tomato microtubule-associated protein end-binding protein 1 (EB1) mediates tolerance to salt stress
作者:
蒲勤华 黄维藻 许春燕 李辉 汪松虎
1中国科学院成都生物研究所 成都 610041 2中国科学院大学 北京 100049
Author(s):
PU Qinhua et al.
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
关键词:
微管正末端结合蛋白周质微管微管解聚药物盐胁迫应答
Keywords:
microtubule plus end binding protein cortical microtubule microtubule depolymerization drug response to salt stress
分类号:
Q943.2 : Q945.78
DOI:
10.19675/j.cnki.1006-687x.2018.03007
摘要:
微管结构的动态受微管结合蛋白(Microtubule-associated proteins,MAPs)调控,在植物的生长发育和环境信号响应中有重要作用. EB1(End-binding protein 1)是微管正末端特异结合的MAP,蛋白同源序列比对搜索显示番茄基因组有2个EB1基因,SlEB1a(Solyc03g116370)和SlEB1b(Solyc02g092950). 构建SlEB1a基因的过表达番茄植株和同时干涉SlEB1a基因和SlEB1b基因的RNAi番茄植株,并分析它们对微管解聚药物戊炔草胺和盐胁迫的敏感性. 结果证明番茄微管结合蛋白EB1(SlEB1)在盐胁迫应答中有重要作用. 与野生型番茄植株相比,过表达番茄植株对1 μmol/L微管解聚药物戊炔草胺更加敏感,而RNAi植株对1 μmol/L戊炔草胺更加耐受,与此相反,过表达番茄植株对100 mmol/L NaCl更加耐受而RNAi番茄植株对100 mmol/L NaCl更加敏感. 因此,SlEB1可能通过负调控番茄周质微管的稳定性而正调控番茄对盐胁迫的应答;本研究结果可为进一步研究植物周质微管动态在盐胁迫应答中的作用机制奠定基础. (图5 参21)
Abstract:
The dynamics of microtubules is regulated mainly by microtubule-associated proteins (MAPs) and plays an important role in plant development and response to environmental signals. End-binding protein 1 (EB1) is a MAP specially binding to the microtubule plus end. Blast search of tomato genome showed two EB1 genes, which were named as SlEB1a (Solyc03g116370) and SlEB1b (Solyc02g092950) in this study. Transgenic tomato plants over-expressing SlEB1a or RNA interfering both SlEB1a and SlEB1b were constructed, and their sensitivity to microtubule depolymerization drug propyzamide and salt stress were analyzed. In this study, we determined the role of tomato EB1 (SlEB1) in the response to salt stress. Compared to the wild-type control plants, OE plants were more sensitive to 1 μmol/L propyzamide, whereas RNAi plants were more tolerant to 1 μmol/L propyzamide; in contrast, OE plants were more tolerant to 100 mmol/L NaCl, whereas RNAi plants were more sensitive to 100 mmol/L NaCl. Thus, SlEB1 might positively regulate salt stress response by negatively regulating the dynamics of tomato cortical microtubules. This study forms a basis for how cortical microtubule dynamics plays a role in plant response to salt stress.

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更新日期/Last Update: 2018-12-25