|Table of Contents|

Cloning and expression analysis of CAD genes under different abiotic stresses in gerbera (PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2021 05
Page:
1390-1398
Research Field:
Articles
Publishing date:

Info

Title:
Cloning and expression analysis of CAD genes under different abiotic stresses in gerbera
Author(s):
GUO Ye1# LI Shenchong2# WANG Xingqi1 FAN Yue1 WU Huan1 LU Zhenhong2 3 YANG Chunmei2 3 & CHENG Chunzhen1?
1 Horticultural Biotechnology, College of Horticulture Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Flower Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China 3 Yuxi Yunxing Biotechnology Co., Ltd., Yuxi 652604, China
Keywords:
gerbera cinnamyl alcohol dehydrogenase gene cloning abiotic stress gene expression
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2020.06054
DocumentCode:

Abstract:
To reveal the sequence characteristics and expression patterns of the cinnamyl alcohol dehydrogenase gene (GjCAD) under different abiotic stress treatments in gerbera. Using reverse transcription PCR (RT-PCR), two GjCADs (GjCAD3 and GjCAD5) genes were successfully cloned from gerbera jamesonii ‘Linglong’. A series of bioinformatics software was used to characterize their nucleotide and protein sequences. Quantitative real-time quantitative PCR (qRT-PCR) was used to show their expression patterns in different tissues and organs (including roots, petioles, and leaves) and under different abiotic stresses (including 4 ℃ low temperature, PEG mimicked drought, salicylic acid (SA), and NaCl treatments). The coding sequences (CDS) of GjCAD3 and GjCAD5 were 1 089 bp and 1 077 bp, respectively. Both their encoded proteins do not contain signal peptides or transmembrane structures. GjCAD3 is a hydrophilic protein belonging to the MDR superfamily, while GjCAD5 is a hydrophobic protein belonging to the PLN02514 superfamily. qRT-PCR results showed that both GjCAD3 and GjCAD5 expressed the highest expression in roots, followed by petioles and leaves. Under low-temperature treatment, the expression of GjCAD3 was significantly induced at all time points except at 24 h (significantly lower than control). In contrast, the expression of GjCAD5 was significantly inhibited at all time points. Under drought treatment, GjCAD3 was significantly suppressed, but GjCAD5 expression at 1 h and 12 h post-treatment was significantly induced. At other time points, its expression was similar to that of the control. Under SA treatment, GjCAD3 was significantly inhibited, and GjCAD5 expression was only significantly higher than that in the control at 24 h post-treatment. Under salt stress, GjCAD3 was significantly inhibited, whereas GjCAD5 was significantly induced. Both GjCAD3 and GjCAD5 were widely involved in gerbera abiotic stress responses, but their functions varied significantly.

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Last Update: 2021-10-25