|Table of Contents|

Effects of lime and ammonium carbonate fumigation coupled with bio-organic fertilizer application on banana fungal community(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2021 05
Page:
1326-1333
Research Field:
Articles
Publishing date:

Info

Title:
Effects of lime and ammonium carbonate fumigation coupled with bio-organic fertilizer application on banana fungal community
Author(s):
LIU Shanshan1 HU Xiaru1 WANG Yunzhou1 LI Chunyu2 TAO Chengyuan1 LI Rong1? & SHEN Qirong1
1Jiangsu Key Laboratory of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China 2Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangdong 510640, China
Keywords:
fumigation bio-fertilizer fungus structure function
CLC:
-
PACS:
DOI:
10.19675/j.cnki.1006-687x.2020.04037
DocumentCode:

Abstract:
Regulation of the soil fungal flora is critical for banana wilt prevention and control. This study aimed to investigate the effect of lime-ammonium fumigation coupled with bio-organic fertilizer application on soil fungal flora in a field experiment. This study focused on the effects of organic fertilizer (OF), bio-organic fertilizer (BF), lime, and ammonium carbonate fumigation coupled with organic fertilizer (LAOF), and lime-ammonium fumigation coupled with bio-organic fertilizer application (LABF) on banana fungal communities using high-throughput sequencing methods based on field experiments. The alpha diversity analysis of soil fungi showed that fumigation combined with different fertilizer applications significantly reduced richness compared with organic and bio-organic fertilizers. The diversity of LABF was significantly lower than that of BF, while there was no significant difference between the LAOF and OF. LABF showed significantly higher richness and diversity than LAOF. Principal coordinate analysis (PCoA) and multivariate regression tree analysis (MRT) of fungal communities based on the Bray-Curtis distance metric showed that soil fumigation and fertilization were influential factors driving the change in fungal community composition. Still, fumigation has a decisive impact on community composition. Moreover, the microbial community structure of LABF was significantly different from that of other treatments, and the community composition differences of LABF and LAOF were greater than those of BF and OF. Network structure analysis results showed that fumigation coupled with different fertilization treatments simplified the network structure of soil fungi. The network of harmful microorganisms in the network structure before fumigation is more complicated, while the network of beneficial microorganisms in the network structure after fumigation is more complicated. This indicated that fumigation changed the microbial network structure from harmful microorganisms to beneficial microorganisms. FUNGuild functional annotation results showed that fumigation significantly reduced the relative abundance of plant pathogens and saprotrophic fungi in the soil and increased the relative abundance of beneficial fungi such as ectomycorrhizal fungi. In addition, compared with LAOF, ectomycorrhizal fungi were significantly enriched in the LABF treatment. Analysis of key nodes in the network revealed that the abundance of OTU_4 (Fusarium) was significantly reduced in fumigation combined with different fertilizers compared with non-fumigation. The relative abundance in LABF was significantly lower than that in LAOF. Spearman correlation analysis showed that there was a significant positive correlation between OTU_4 and disease incidence. OTU_1 (Tuber), OTU_14 (Cordycipitaceae_unidentified), and OTU_21 (Simplicillium) were significantly enriched in LAOF and LABF, and the relative abundance of these microbes was also significantly and negatively correlated with disease incidence. In conclusion, lime and ammonium fumigation coupled with the application of bio-organic fertilizer reduced the richness and diversity of soil fungi. It enriched the key taxa with disease suppression potential, resulting in a healthy and beneficial soil fungal community.

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Last Update: 2021-10-25