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Response of heterologous overexpression of Populus euphratica PeGRF6/8a in tobacco under different stresses(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 03
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Response of heterologous overexpression of Populus euphratica PeGRF6/8a in tobacco under different stresses
FENG Qian CHEN Yongfu YAO Yin’an WU Yingqing ZHANG Guoyang HAN Ying & GAO Yongfeng**
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
PeGRF6/8a 14-3-3 protein abscisic acid tobacco low nitrogen high nitrogen salt stress
Q945.78 : Q78

14-3-3 Protein is a type of highly conserved protein family widely distributed both in animals and plants. It is not only involved in various physiological and biochemical processes and metabolic reactions in organisms by interacting with different target proteins but also plays important roles in response to biotic and abiotic stresses. To clarify the function of the PeGRF6/8a under abiotic stress, we cloned the PeGRF6/8a from the 14-3-3 protein family of Populus euphratica, constructed pBI121-35S::PeGRF6/8a, an expression vector, and then transformed it into wild-type tobacco by Agrobacterium-mediated transformation. The obtained transgenic tobaccos were treated as follows: (1) Seed germination experiments under 1 μmol/L and 2.5 μmol/L abscisic acid (ABA) treatments; (2) Root length comparison experiments under low nitrogen (2.5 mmol/L KNO3) and high nitrogen (150 mmol/L KNO3) treatments; (3) 1/2 Hoagland hydroponics experiments—four treatments that included control (CK), low nitrogen (0.2 mmol/L KNO3), high nitrogen (150 mmol/L KNO3), and salt stress (150 mmol/L NaCl). The results were as follows: (1) Under ABA treatment, the germination rate of transgenic tobacco seeds was lower than that in the wild type. Upon increased ABA concentration, the germination rate of transgenic tobacco seeds declined much faster than that of the wild type; (2) Under low nitrogen treatment, the root length of transgenic tobacco was distinctly shorter than that of wild type tobacco. However, it was in contrast with the root length under high nitrogen treatment. (3) Under low nitrogen and salt treatments, compared to the wild type, in transgenic tobacco, the lower leaves showed yellowing or wilting and the chlorophyll and carotenoid content in the leaves were markedly lower. Furthermore, the activity of superoxide dismutase and osmolyte content such as proline and soluble proteins was significantly lower in transgenic than in wild-type plants but the malondialdehyde (MDA) content was higher. However, under high nitrogen treatment, the speed of plants wilting in the wild type was markedly faster than that in the transgenic plants, and the resistance and related physiological indexes (except MDA) of transgenic plants were considerably higher than those of wild type. These results indicate that heterologous overexpression of P. euphratica PeGRF6/8a in tobacco can weaken plant tolerance to low nitrogen and salt stresses, but it can enhance plant tolerance under high nitrogen stress.


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Last Update: 2019-06-25