|Table of Contents|

Influence of long-term manure application in paddy soil on the functional diversity of microbial community

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 03
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Influence of long-term manure application in paddy soil on the functional diversity of microbial community
GUO Ying1 2 WANG Yiming1** WU Pan1 & PENG Shuang1
1 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 2 University of Chinese Academy of Sciences, Beijing 100049, China
Biolog edaphon manure application long-term fertilization carbon analysis

To make the influence of long-term manure application on carbon turnover clear, and provide references for rational manure utilization, as well as improvement of soil fertility, the diversity of the microbial community, and carbon source utilization in paddy soil (0–40 cm) were studied. Tests were conducted by Biolog, after long-term fertilization under different treatments (with low/high level of fresh/composted manure application) at the Changshu Agro-ecological Experimental Station, of the Chinese Academy of Sciences. Statistical analyses showed that both the carbon source utilization by microbes and diversity indexes (Shannon, Simpson, and McIntosh) in fertilization treatments were significantly higher than those with CK treatment in the 0–20 cm plough layer, whereas there were no differences in the 20–40 cm layer. The microbial activity of fertilized treatments was significantly correlated with the content of soil organic matter and total nitrogen. Factor analysis indicated that different fertilization treatments could be recognized by various carbon utilization patterns, which were mainly explained by principal component I, and the variance contribution rate is 71.31%. Carbohydrates and amphiphilic were the dominant carbon sources that lead to the differentiation between applying manure and non-fertilizer treatments and were mainly affected by β-methyl-D-glucoside, N-acetyl-D-glucosamine, α-D-lactose, and pyruvic acid methyl ester. Principal component analysis showed that soil samples of various depth could be differentiated by principle component I, which explained the alteration of carbon source utilization (with a variance contribution rate of 71.67%) and the difference among points in each soil layer reached a significant level (P < 0.01). Carbohydrates and carboxylic acid were the dominant carbon sources contributing to PCAI, which were mainly affected by β-methyl-D-glucoside, N-acetyl-D-glucosamine, α-D-lactose, and D-xylose. Additionally, the differences in carbon source utilization types caused by fresh and fermented manure were mainly reflected in the 5–10 cm soil layer. For the group with low-quantity manure application, composted manure treatments had a higher utilization rate for polymers and carbohydrates than did the fresh ones, whereas in the high-amount fertilization group the utilization rates of amino acids and amides were higher in composted ones than that of fresh manure application with a more highly significant difference. Although there were differences between fresh and fermented pig manure, as well as different application amounts, the long-term application of fresh and fermented pig dung is helpful in the improvement of carbon source utilization capacity and community diversity of soil microorganisms; thus, enhancing the conversion and turnover capacity of soil organic matter. Therefore, the rational application of organic fertilizers, such as manure, is of great significance to the improvement of soil organic matter, the conservation of farmland, and the reclamation of livestock manure.


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Last Update: 2019-06-25