|Table of Contents|

Screening and identification of lead and cadmium resistant microorganisms from combined heavy metal pollution soil(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 03
Page:
532-538
Research Field:
Publishing date:

Info

Title:
Screening and identification of lead and cadmium resistant microorganisms from combined heavy metal pollution soil
Author(s):
XU Shaohui1 JIANG Daihua1 2** SHI Dingding1 ZHANG Rongrong1 HUANG Zhigang1 2 & YANG Gairen3
1 Agricultural College, Guangxi University, Nanning 530004, China 2 Guangxi Key Laboratory of Agricultural Environment and Agricultural Product Safety Cultivation Base, Nanning 530004, China 1 Forest College of Guangxi University, Nanning 530004, China
Keywords:
heavy metal pollution lead and cadmium resistant microorganism screening identification
CLC:
X172
PACS:
DOI:
10.19675/j.cnki.1006-687x.201808028
DocumentCode:

Abstract:
To obtain the microbial resource strains with high heavy metal compound tolerance to remedy heavy mental contaminated soil, soil samples were collected from the tail mining area in Northwest Guangxi. Improved Martin’s medium was used to isolate and screen the high-tolerance microorganisms for heavy metal lead and cadmium. The fungus with compound tolerance to lead and cadmium termed K-3 was obtained, whose lead resistance was 1 200 mg/L and cadmium resistance was 120 mg/L. The optimum temperature for K-3 was 30 ℃, pH was 7.0, and salt concentration was 3%. The identification of its morphology and molecular biology showed that the sequence consistency of 18S rDNA between K-3 and the standard strain Uncultured Westerdykella was 99%. By consulting relevant literature and comprehensive inference, it was shown that K-3 was one of the strongest double-tolerance fungi for heavy metal lead and cadmium. It provided precious microbial germplasm resources for remediation of heavy metal contaminated soil using microorganisms or microorganism-associated plants.

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Last Update: 2019-06-25