|Table of Contents|

Effects of activated sludge-extracted extracellular polymeric substances on the aggregation of Chlamydomonas microsphaera

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 03
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Effects of activated sludge-extracted extracellular polymeric substances on the aggregation of Chlamydomonas microsphaera
SHI Huasheng1 SHEN Renhao1 HU Zhen1 & CHEN Guowei1 2**
1 School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei 230009, China 2 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China
microalgae aggregation activated sludge extracellular polymeric substances (EPSs) cell motility aromatic protein tryptophan protein

Though extracellular polymeric substances (EPSs) play important roles in the aggregation of activated microbial sludge, little is known about their effects on the aggregation of other, non-bacterial species. In this study, the interaction between activated sludge-extracted EPSs and microalgal cells (Chlamydomonas microsphaera) was investigated. Image and video analyses were applied to track microalgal aggregation and motility. The characteristics of the EPSs used and the self-responses of the tested microalgae were also measured. Results showed that with the application of increasing amounts of exogenous EPSs, the aggregation rate of Chlamydomonas microsphaera increased from 18.4 to 81.8%, with the maximum aggregation rate reached with the application of 110 mg/L of EPSs. In the presence of activated sludge-extracted EPSs, microalgal cells moved faster, with their speed increasing from 26.8 to 49.4 μm/s, which caused there to be higher probabilities of collision among microalgal cells that favored microalgal aggregation. The results of EEM also showed that the EPS content of the activated sludge was proportional to that of the aromatic and tryptophan proteins secreted by the microalgae, which implied that the sludge-extracted EPSs stimulated the secretion of aromatic and tryptophan proteins by the microalgae. However, the zeta potential of the exogenous EPS solution was negatively correlated with the aggregation rate of the microalgae, indicating that zeta potential changes were not responsible for the accumulation of microalgal cells. In conclusion, it was found that sludge-extracted EPSs enhanced the aggregation of microalgae.


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Last Update: 2019-06-25