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Effects of cadmium stress on cyanide-resistant respiration, antioxidation, and PS II photosynthetic characteristics of kidney bean leaves(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 03
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Effects of cadmium stress on cyanide-resistant respiration, antioxidation, and PS II photosynthetic characteristics of kidney bean leaves
XIE Jiajia SAINAO Wangqing RAN Ruilan ZHANG Mudan JIA Lingyun & FENG Hanqing**
College of Life Science, Northwest Normal University, Lanzhou 730070, China
cyanide-resistant respiratory pathway cadmium stress PS II antioxidation kidney bean plant

To explore the protective effects and possible mechanisms of cyanide-resistant respiration (alternating pathway) of plants under cadmium stress, we investigated the effects of cadmium stress on cyanide-resistant respiration, antioxidation, and photosynthetic characteristics of photosystem II (PS II) in kidney bean leaves. We determined whether the effects of cyanide-resistant respiration on PS II is related to its involvement in antioxidant regulation. The results showed that the ability of cyanide-resistant respiration, the activity of antioxidant enzymes [including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX)] and the content of hydrogen peroxide (H2O2) first increased but then decreased with the increase in cadmium concentration, whereas the superoxide anion radical (O2·-) content significantly increased with the increase in cadmium concentrations. Simultaneously, the effective photochemical quantum yield of PS II photosynthetic [Y(II)], photochemical quenching coefficient (qP), and rate of non-cyclic electron transport through PS II (ETR) of the leaves decreased. However, the non-photochemical quenching (NPQ) of leaves increased with the increase in cadmium concentration. Under conditions without cadmium stress, spraying with exogenous eliminator of H2O2 and O2·? or inhibitor of cyanide-resistant respiration on the leaf surface did not adversely affect chlorophyll fluorescence parameters, but qP and ETR significantly increased with the application of H2O2 eliminator. Under cadmium stress, treatment with the exogenous eliminator of H2O2 and O2·? could effectively alleviate the decrease in photochemical activity of PS II, whereas treatment with the inhibitor of cyanide-resistant respiratory pathway caused further decrease in PS II photochemical efficiency, increase in reactive oxygen species level, and decrease in antioxidant enzyme activities. The results showed that cyanide-resistant respiration can effectively alleviate the excessive accumulation of reactive oxygen species, increase of antioxidant enzyme activity, and decrease the PS II photochemical activity in the kidney bean seedling leaves caused by cadmium stress. Furthermore, the function of the cyanide-resistant respiratory pathway in protecting the PS II from Cd stress could be related to the ability of the cyanide-resistant respiratory pathway to regulate the antioxidant capacity of plants.


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Last Update: 2019-06-25