|Table of Contents|

Effects of mercuric chloride on the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the CYP2 subfamily in the heart and pancreas of the* Chinese giant salamander, Andrias davidianus

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 03
Page:
546-552
Research Field:
Publishing date:

Info

Title:
Effects of mercuric chloride on the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the CYP2 subfamily in the heart and pancreas of the* Chinese giant salamander, Andrias davidianus
Author(s):
YE Ting LIU Ya’nan WANG Xingxiang CAO Yu XU Kangkang & LI Can**
College of Biology and Environmental Engineering, Guiyang University / Guizhou Provincial Key Laboratory for Rare Animal and Economic Insects of the Mountainous Region, Guiyang 550005, China
Keywords:
Andrias davidianus mercuric chloride cytochrome P450 2K1 (CYP2K1) cytochrome P450 2F3 (CYP2F3) cytochrome P450 2F5 (CYP2F5) cytochrome P450 2C19 (CYP2C19)
CLC:
X174: X171.5
PACS:
DOI:
10.19675/j.cnki.1006-687x.201807025
DocumentCode:

Abstract:
The effects of mercuric chloride (HgCl2) on the expression of the cytochrome P450 2K1 (CYP2K1), cytochrome P450 2F3 (CYP2F3), cytochrome P450 2F5 (CYP2F5), and cytochrome P450 2C19 (CYP2C19) genes in the cytochrome P450 2 (CYP2) subfamily in the heart and pancreas of the Chinese giant salamander, Andrias davidianus, were investigated in this study to provide basic data for evaluating the ecotoxicological effects of mercuric chloride on this species. Juvenile giant salamanders were continuously exposed to different concentrations of HgCl2 (0, 1, 10, and 100 ng/L) for 24, 48, and 72 h, and the effects of mercuric chloride on the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in their heart and pancreas were investigated using real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). After exposure to HgCl2 for 24 h, the expression levels of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the hearts of giant salamanders were increased compared with those in the control group (exposed to 0 ng/L HgCl2). In the hearts of giant salamanders exposed to 1 ng/L HgCl2 for 24 h, the expression levels of the CYP2K1 and CYP2C19 genes were significantly increased (P < 0.05), while exposure to 10 ng/L HgCl2 for 24 h significantly increased CYP2F3 and CYP2F5 gene expression levels (P < 0.05), and CYP2K1, CYP2F3, CYP2F5, and CYP2C19 gene expression levels were all significantly increased by exposure to 100 ng/L HgCl2 for 24 h (P < 0.05). In the pancreases of giant salamanders, the expression levels of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes tended to be lower than those in the control group after exposure to HgCl2 for 24 h. Exposure to both 10 and 100 ng/L HgCl2 for 24 h significantly inhibited the expression of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the pancreas (P < 0.05). After exposure to HgCl2 for 48 h, the expression levels of CYP2F5 in the hearts of giant salamanders significantly decreased in a dose-dependent manner (P < 0.05), and significantly elevated CYP2F3 expression was found in the pancreas (P < 0.05). After exposure to HgCl2 for 72 h, there were no significant changes in the expression levels of the CYP2K1, CYP2F3, CYP2F5, and CYP2C19 genes in the hearts and pancreases of giant salamanders compared with those in the control treatment (P > 0.05). It was concluded that no significant change in the expression of CYP2 subfamily genes occurred with increasing exposure time, which suggested that the responses of the CYP2 subfamily genes CYP2K1, CYP2F3, CYP2F5, and CYP2C19 in the heart and pancreas of the Chinese giant salamander may be converted into a chronic cumulative toxic effect in response to exposure to mercuric chloride exposure.

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Last Update: 2019-06-25