|Table of Contents|

Expression characteristics of 15 miRNAs and their candidate target genes in Oncidium hybridum(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2019 01
Page:
108-106
Research Field:
Articles
Publishing date:

Info

Title:
Expression characteristics of 15 miRNAs and their candidate target genes in Oncidium hybridum
Author(s):
WANG Peiyu LIN Zhengchun WANG Congqiao GAO Yuying CHEN Yukun YE Wei LAI Zhongxiong** & LIN Yuling**
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Keywords:
Oncidium hybridum miRNAs soft rot target expression analysis
CLC:
Q949.718.43: Q78
PACS:
DOI:
10.19675/j.cnki.1006-687x.2018.08018
DocumentCode:

Abstract:
This study aimed to investigate the response mode of miRNA and candidate target genes in the disease resistance process of Oncidium. The transcriptome data and miRNA database of Oncidium leaves infected by soft rot (normal, mild, and severe infection) pathogens were analyzed. Subsequently, 15 miRNAs were screened out, and their candidate targets and functional annotations were predicted by bioinformatics analysis. A real-time quantitative PCR analysis was applied to clarify the expression of miRNAs and their candidate targets in different tissues and pseudobulbs infected by soft rot. An miRNA read analysis based on the Oncidium miRNA database suggested that different miRNAs might have different functions in normal plants and in different stages of Oncidium susceptibility. Using the psRNATarget online software to predict the candidate targets of Oncidium miRNAs, we found 828 candidate targets for 15 miRNAs in Oncidium; among them, 67 targets, including serine/threonine-protein, F-box protein gene, zinc finger protein, resistance gene analog (RGA) protein, might be related to disease resistance. Based on the miRNA reads and the reads per kilobase per million mapped reads (RPKM) values of candidate targets, the miRNAs and their candidate targets were found to be differentially expressed in normal plants and in different susceptible periods of Oncidium. A real-time quantitative PCR analysis showed that miR159a, miR167b, miR168a, miR169a, miR171a, and miR172a were abundantly expressed in the pseudobulbs and leaves of Oncidium and were negatively correlated with the expression of the corresponding candidate targets, suggesting that they are involved in the development and morphogenesis of pseudobulbs and leaves. In the Oncidium pseudobulbs infected by soft rot pathogen, the miRNAs miR159a, miR168a, miR169a, miR171a, and miR172a were highly expressed at 8 h and showed a negatively regulated relationship with the expression of the corresponding candidate targets at 0–8 h of infection. Thus, it was speculated that the above miRNAs respond to the infection process of the soft rot pathogens in the medium term through negatively regulated candidate targets. However, miR167b was highly expressed at 0 h of infection and showed negatively regulated relationship with candidate targets at 0–8 h of infection, suggesting that it is involved in the response of soft rot pathogen infected process by down-regulating its expression. The above research shows that miRNAs might be widely involved in the development of different tissues of Oncidium by mediating the cleavage of candidate targets and response of the infection process of soft rot pathogens.

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Last Update: 2019-02-25