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Thermophilic fermentation of syngas to acetate by mixed cultures and their microbial community(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 01
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Thermophilic fermentation of syngas to acetate by mixed cultures and their microbial community
LU Shuailing1 ZHU Hui1 FU Bo1 2** LIU Hongbo1 2 & LIU He1 2
1 School of Environmental and Civil Engineering, Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi 214122, China 2 Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215009, China
syngas fermentation mixed culture acetate thermophiles microbial community
Q939.9 : TQ920

Syngas is a mixture of mainly CO, H2, and CO2, which can be produced from the gasification of various organic materials. Syngas fermentation has attracted great attention for its ability to produce valuable biofuels and biochemicals. As biocatalysts for syngas fermentation, microorganisms are important to product profile and conversion efficiency. In this research, the thermophilic CO-, formate- and syngas-converting microorganisms were enriched from cow manure, and the capability for acetate production from syngas by the CO- and formate-converting bacteria mixture and syngas-converting bacteria, as well as their microbial communities, were analyzed. The results showed that the CO-driven hydrogenogenesis and acetogenesis occurred during the initial stage of syngas fermentation and resulted in the formation of H2 + CO2 and acetate, respectively. The CO conversion efficiency was 100%, and the CO conversion rates of the CO- and formate-converting bacteria mixture and syngas-converting bacteria were 6.93 and 6.34 mmol L-1 d-1, respectively. Subsequently, H2 + CO2 was mainly converted into acetate via the acetogenic Wood-Ljungdahl pathway, and the final accumulation of acetate for the Co-and formate-converting bacteria mixture and syngas-converting bacteria were 9.11 and 8.01 mmol/L, respectively. The bacterial genera Thermoanaerobacterium, Romboutsia, Ruminococcus, Clostridium, Eubacterium, Moorella, and Desulfotomaculum dominated in the CO- and formate-converting bacteria mixture, and Romboutsia, Thermoanaerobacterium, Moorella, Eubacterium, Acetonema, and Clostridium were the main genera comprising the syngas-converting bacteria; among these genera, Ruminococcus, Clostridium, Eubacterium, Moorella and Acetonema harbor known homoacetogenic species. It is shown that the mixture of CO- and formate-converting bacteria can be used for thermophilic syngas fermentation, and its conversion ability is higher than that of the syngas-converting bacteria. This study identified potential microorganism resources and provides technical references for valuable chemicals and bioenergy production by syngas mixed-culture fermentation.


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Last Update: 2019-02-25