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Effects of trace elements on microbial community structure in continuous anaerobic digestion of vegetable waste(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 01
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Effects of trace elements on microbial community structure in continuous anaerobic digestion of vegetable waste
SUN Juan1 LI Dong2** ZHENG Tao1** LIU Xiaofeng1 CHEN Lin1 & HE Mingyang1
1 Institute of Urban and Rural Mines, Changzhou University, Changzhou 213164, China 2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
vegetable waste anaerobic digestion trace element microbial community structure

To optimize the performance of continuous anaerobic digestion of vegetable waste, the relationship between trace elements and microbial community structure was explored. In this study, the continuous anaerobic digestion (AD) of vegetable waste (VW) was carried out in the 70-L anaerobic reactors under mesophilic conditions (35 ℃), and the trace element supplementation was based on the CH4 content in order to stabilize the process under high organic loading rate (OLR) for a long run-length. The microbial community structure was obtained through MiSeq sequencing of 16S rRNA gene amplicons. For the bacterial communities, the addition of trace elements had significant effects on Bacteroidetes, Spirochaetae, and Firmicutes. At the genus level, the first trace elements addition induced VadinBC27 wastewater-sludge to increase from 54.1% to 68% and reduced Erysipelotrichaceae UGG-004 and Sphaerochaeta. The first trace element addition induced the abundance of Bacteroides to increase, reduced that of Sphaerochaeta from 11.4% to 4.4% to improve the utilization of raw materials, and reduced the inhibition of acidification. For the methanogen communities, the addition of trace elements had significant effects on Candidatus Methanoplasma, which is a methantrophic-utilization methanogen and acetic-utilization methanogens with Methanosaeta dominated. Candidatus Methanoplasma dominated in the presence of high volatile fatty acids (VFA), and Methanosaeta dominated from 2.3% to 80% after the addition of trace elements to promote the consumption and conversion of VFA. Therefore, trace elements can promote the balance of hydrolysis and acidification and methanation in anaerobic digestion of vegetable waste, thus stabilizing the operation.


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Last Update: 2019-02-25