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Clay-cultured aerobic granular sludge and its use in the treatment of tomato-paste processing wastewater(PDF)

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

2019 01
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Clay-cultured aerobic granular sludge and its use in the treatment of tomato-paste processing wastewater
FENG Dianbao1 WANG Weihong1** WANG Yanshan1 & SU Kuizu 2
1 College of Hydraulic and Civil Engineering, Xinjiang Agricultural University, Urumqi 830052, China 2 School of Civil Engineering, Hefei University of Technology, Hefei 230009, China
tomato paste processing wastewater aerobic granular sludge clay high-throughput sequencing

The aerobic granular sludge technology was adopted to treat tomato-paste processing wastewater in Xinjiang because it receives high amount of discharge products and high concentration of organic matter. A sequencing batch reactor (SBR) was operated to investigate the effect of clay augmentation on aerobic granulation. Flocculent sludge and clay were fed into the synthetic tomato-paste processing wastewater contained in the SBR. The combined bio-analytical techniques of scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), live/dead staining, and high-throughput sequencing were used to characterize the granulation process of activated sludge. The results showed that the average particle size of the granules in the clay-added reactor was approximately 0.54 mm in 20 days. After maturation, the granular sludge had a good settling ability, and the average removal rate of COD, NH4+-N, and PO43--P reached 90%, 85%, and 40%, respectively. The SEM results showed that the granules had a clear outline and compacting structure. The CLSM results revealed that α-D-glucopyranose polysaccharide, β-D-glucopyranose polysaccharide, and protein, as components of extracellular polymeric substances (EPS), were widely distributed throughout the granule. The live/dead bacterial staining images of the clay-added granular sludge showed a high accumulation of dead bacteria. The live bacteria were distributed more closely towards the edge of the granule, while the dead bacteria were trapped inside. The high-throughput sequencing results showed that the addition of clay led to an increase in microbial diversity, with the Shannon index increasing from 4.50 to 4.79. The relative abundances of the aerobic bacterial genera Zoogloea and Flavobacterium were 37.87% and 8.79%, respectively. The study demonstrated that the addition of clay could accelerate the formation of aerobic granular sludge, and the maintenance of the stability of the system and degradation of organic matter mainly resulted from the combined action of microbial communities.


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Last Update: 2019-02-25