|Table of Contents|

Cloning and expression of the copper/zinc-superoxide dismutase 1 gene and its promoter under low temperature stress in Camellia sinensis

Chinese Journal of Applied & Environmental Biology[ISSN:1006-687X/CN:51-1482/Q]

Issue:
2018 05
Page:
1122-1131
Research Field:
Articles
Publishing date:

Info

Title:
Cloning and expression of the copper/zinc-superoxide dismutase 1 gene and its promoter under low temperature stress in Camellia sinensis
Author(s):
GUO Yuqiong1 WANG Zhong1 2 ZHU Chen1 2 ZHAO Shanshan1 ZHANG Shuting1 2 CHANG Xiaojun1 2 LI Xiaozhen1 FU Haifeng1 LIN Yuling1 2 & LAI Zhongxiong1 2**
1 College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2 Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3 College of Food and Biological Engineering, Hezhou University, Hezhou 542899, China
Keywords:
Camellia sinensis copper/zinc-superoxide dismutase 1 (CSD1) gene low-temperature stress expression analysis
CLC:
S571.1 : Q78
PACS:
DOI:
10.19675/j.cnki.1006-687x.2018.02021
DocumentCode:

Abstract:
The growth and quality of tea are severely?affected by low temperature. Copper/zinc-superoxide dismutase (CSD) genes play crucial roles in the response of plants to stress. The objective of this study was to explore the response mechanism of CSD gene in tea (CsCSD) under low temperature stress. The leaves of Camellia sinensis “Tieguanyin” were used as raw material. The full-length cDNA (GenBank accession number: KR078346), gDNA, and promoter sequence of CsCSD1 were cloned using homology-based cloning, combined with RACE and chromosome walking. Moreover, the bioinformatic characteristics and expression patterns of CsCSD1 under low temperature stress were investigated. The results showed that the full-length cDNA of CsCSD1 was 860 bp long, containing a 459 bp open reading frame (ORF) which encodes a protein of 152 amino acids. The gDNA structural analysis found that the CsCSD1 gene was composed of 6 exons and 5 introns. In addition, the CpG island prediction found that there was only a CpG island in the promoter region. The length of the CpG island was 219 bp and the GC content was 50.3%. The CsCSD1 promoter also contained a large number of cis-acting elements, including light response elements, hormone response elements, stress response elements, and other response elements. Expression analysis revealed that the expression of CsCSD1 was up-regulated at the early stage of low temperature stress, and then the expression decreased continuously. This study showed that the CsCSD1 gene can respond to low temperature stress and play different roles in different periods of low temperature stress.

References

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